张殿昌, 黄燕琴, 苏天凤, 李建柱, 朱彩艳, 江世贵. 重组鲮GH对鲮IGF-Ⅰ表达的影响[J]. 南方水产科学, 2008, 4(2): 50-55.
引用本文: 张殿昌, 黄燕琴, 苏天凤, 李建柱, 朱彩艳, 江世贵. 重组鲮GH对鲮IGF-Ⅰ表达的影响[J]. 南方水产科学, 2008, 4(2): 50-55.
ZHANG Dianchang, HUANG Yanqin, SU Tianfeng, LI Jianzhu, ZHU Caiyan, JIANG Shigui. The effect of recombinant growth hormone(GH) on insulin-like growth factor-Ⅰ(IGF-Ⅰ) expression in mud carp, Cirrhinus molitorella[J]. South China Fisheries Science, 2008, 4(2): 50-55.
Citation: ZHANG Dianchang, HUANG Yanqin, SU Tianfeng, LI Jianzhu, ZHU Caiyan, JIANG Shigui. The effect of recombinant growth hormone(GH) on insulin-like growth factor-Ⅰ(IGF-Ⅰ) expression in mud carp, Cirrhinus molitorella[J]. South China Fisheries Science, 2008, 4(2): 50-55.

重组鲮GH对鲮IGF-Ⅰ表达的影响

The effect of recombinant growth hormone(GH) on insulin-like growth factor-Ⅰ(IGF-Ⅰ) expression in mud carp, Cirrhinus molitorella

  • 摘要: 使用半定量RT-PCR方法,研究了鲮(Cirrhinus molitorella)各组织中IGF-ⅠmRNA组织表达,并分析重组鲮GH处理后鲮IGF-Ⅰ表达变化情况。结果表明,鲮IGF-ⅠmRNA在肝组织中表达最高,其次是肾和脑,另外在肠、鳃、脾、性腺和心脏组织中也有表达,而在肌肉、皮肤中没有检测到鲮IGF-ⅠmRNA的表达;按120 gg-1鱼体重的剂量经腹腔注射重组鲮GH,观察12 h后鲮IGF-Ⅰ表达变化情况,发现经重组鲮GH处理后,鲮肝组织IGF-ⅠmRNA表达水平显著升高,脑组织IGF-ⅠmRNA表达水平也稍有升高,而在肌肉中仍未检测到IGF-ⅠmRNA的表达;重组鲮GH处理前后,对照组中鲮血清中IGF-Ⅰ的含量为145.5921.84 ngmL-1,试验组中鲮血清中IGF-Ⅰ的含量为247.712.83 ngmL-1,经t检验,P=0.0430.05,表明重组鲮GH处理前后鲮血清中IGF-Ⅰ的含量存在显著性差异。

     

    Abstract: Expression patterns of IGF-ⅠmRNA in different tissues of mud carp (Cirrhinus molitorella)were studied using semi-quantitative RT-PCR method and the effects of recombinant mud carp growth hormone(rmcGH)on expression of IGF-Ⅰin mud carp were investigated. The IGF-ⅠmRNA was detected successfully in liver, kidney, brain, intestine, gill, spleen, gonad, and heart, and no IGF-ⅠmRNA was detected in skin and muscle. The IGF-Ⅰ mRNA detected was higher in liver than in other tissues. Mud carp were received either intra-peritoneal injections of rmcGH (120 gg-1 body weight) or vehicle, tissue samples and blood were collected 12 hour later. Total RNA was isolated and assayed for IGF-ⅠmRNA using semi-quantitative RT-PCR. Blood was extracted to determine the levels of IGF-Ⅰ in serum. IGF-Ⅰlevels increased from 145.5921.84 to 247.712.83 ngmL-1 (P=0.043) in the serum after rmcGH injection.

     

/

返回文章
返回