5种石斑鱼遗传差异的RAPD分析

区又君; 吴勇; 李加儿; 刘楚吾

5种石斑鱼遗传差异的RAPD分析

RAPD analysis of genetic difference among five species of grouper

摘要

摘要: 运用随机扩增多态性DNA（random amplified polymorphic DNA，RAPD）技术对鞍带石斑鱼（Epinephelus lanceolatus）、驼背鲈（Cromileptes altivelis）、棕点石斑鱼（E.fuscoguttatus）、斜带石斑鱼（E.coioides）和鲑点石斑鱼（E.fario）的遗传多样性及系统发生进行了研究。从120个RAPD随机引物中筛选出扩增效果好的引物19个，分别用于5种石斑鱼基因组DNA的扩增。结果显示，5种石斑鱼平均多态性位点比率（P）分别为64.23%，72.61%，60.34%，69.97%，73.94%；个体间平均遗传相似系数（S）分别为0.8238，0.8110，0.8345，0.8277，0.8064；平均遗传距离（D）分别为0.1762，0.1890，0.1655，0.1723，0.1936；平均Nei基因多样性指数（H）分别为0.1189，0.1364，0.1028，0.1439，0.1648；平均Shannon信息指数（Hi）分别为0.1801，0.1992，0.1530，0.2100，0.2434。5个种间的遗传距离（Dxy）在0.3964~0.6085之间。

Abstract: Genetic diversity and molecular evolution of five species of grouper, viz. Epinephelus lanceolatus, Cromileptes altivelis, E.fuscoguttatus, E.coioides and E.fario, were detected using RAPD technique. A total 120 random primers were evaluated for the usefulness. And thereinto, 19 random primers were selected according to the effect of PCR of genomic DNA of 5 species of grouper. The results showed as the followings: the polymorphic loci ratio in E.lanceolatus, C.altivelis, E.fuscoguttatus, E.coioides and E.fario (P) were 64.23%, 72.61%, 60.34%, 69.97% and 73.94%, respectively. The mean genetic similarity index of intra-species (S) were 0.8238，0.8110，0.8345，0.8277，0.8064, respectively, the mean genetic distances of intra-species (D) were 0.1762, 0.1890, 0.1655, 0.1723 and 0.1936, respectively, Nei's gene diversity (H) were 0.1189, 0.1364, 0.1028, 0.1439 and 0.1648, respectively, and Shannon's Information index (Hi) were 0.1801, 0.1992, 0.1530, 0.2100, 0.2434, respectively. The mean genetic distances of inter-species (Dxy) were between 0.3964~0.6085.

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