刘栋梁, 黄建华, 周发林, 杨其彬, 姜松, 杨丽诗, 朱彩艳, 江世贵. 维生素E和裂壶藻对斑节对虾精荚再生的协同作用[J]. 南方水产科学, 2018, 14(1): 20-26. DOI: 10.3969/j.issn.2095-0780.2018.01.003
引用本文: 刘栋梁, 黄建华, 周发林, 杨其彬, 姜松, 杨丽诗, 朱彩艳, 江世贵. 维生素E和裂壶藻对斑节对虾精荚再生的协同作用[J]. 南方水产科学, 2018, 14(1): 20-26. DOI: 10.3969/j.issn.2095-0780.2018.01.003
shu
LIU Dongliang, HUANG Jianhua, ZHOU Falin, YANG Qibin, JIANG Song, YANG Lishi, ZHU Caiyan, JIANG Shigui. Synergistic effect of Vitamin E and Schizochytrium sp. on spermatophore regeneration in Penaeus monodon[J]. South China Fisheries Science, 2018, 14(1): 20-26. DOI: 10.3969/j.issn.2095-0780.2018.01.003
Citation: LIU Dongliang, HUANG Jianhua, ZHOU Falin, YANG Qibin, JIANG Song, YANG Lishi, ZHU Caiyan, JIANG Shigui. Synergistic effect of Vitamin E and Schizochytrium sp. on spermatophore regeneration in Penaeus monodon[J]. South China Fisheries Science, 2018, 14(1): 20-26. DOI: 10.3969/j.issn.2095-0780.2018.01.003
shu

维生素E和裂壶藻对斑节对虾精荚再生的协同作用

Synergistic effect of Vitamin E and Schizochytrium sp. on spermatophore regeneration in Penaeus monodon

    摘要
  • 摘要: 文章探究了维生素E (Vitamin E, VE)和裂壶藻(Schizochytrium sp., Sch)对斑节对虾(Penaeus monodon)雄虾精荚再生的作用。以基础饲料配制了4种不同配合饲料(0添加组；VE 200组；1% Sch组；VE 200+1% Sch组)，饲喂被挤压排出精荚的成熟雄性斑节对虾40 d，评估斑节对虾雄虾精荚再生后的精子数量和质量。结果表明，添加量为200 mg·kg–1 VE饲料组的活精子数百分比显著高于对照组(P<0.05)；单一添加1%裂壶藻饲料组的活精子数与对照组间差异不显著(P>0.05)；共同添加200 mg·kg–1 VE和1%裂壶藻的饲料组的精子总数显著高于对照组(P<0.05)。综合精荚质量、精子数量及活精子数百分比3个指标得出，饲料中共同添加1%裂壶藻与200 mg·kg–1 VE对斑节对虾雄虾精荚的再生和提高精子数量具有协同促进作用。

     

    Abstract: To investigate the effects of Vitamin E and Schizochytrium sp. on the spermatophore regeneration in Penaeus monodon, we designed four different contents of Vitamin E and Schizochytrium sp. in the basal diet (control group; VE 200; 1% Schizochytrium sp.; VE 200+1% Schizochytrium sp.) to feed mature male P.monodon which had been extruded spermatophore for 40 d. The results show that the percentage of viable spermatozoa added in 200 mg·kg–1 Vitamin E diet group was significantly higher than that in the control group (P<0.05). The sperm quantity of single added in 1% Schizochytrium sp. diet group was not significantly different with control group (P>0.05). The total number of spermatozoa fed with 200 mg·kg–1 of Vitamin E and 1% Schizochytrium sp. was significantly higher than that of the control group (P<0.05). According to the spermatophore quality, sperm quantity and living sperm percentage, adding 200 mg·kg–1 of Vitamin E and 1% Schizochytrium sp. in feed can promote the spermatophore regeneration in the male P.monodon and improve the sperm quantity effectively.

     

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