斑节对虾GRP94基因的克隆及其在不同应激条件下的表达与分析

Characterization and expression analysis of GPP94 in black tiger shrimp (Penaeus monodon) under different acute stresses

摘要: 以斑节对虾(Penaeus monodon)为研究对象, 利用RACE 技术克隆得到了斑节对虾PmGRP94基因cDNA全长, 并对其结构进行生物信息学分析。PmGRP94全长2 990 bp, 包括75 bp的5UTR、527 bp的3UTR和2 388 bp的ORF。利用实时定量PCR技术, 对PmGRP94组织特异性及其在不同pH、盐度和3种重金属应激下转录水平变化情况及特点进行了研究。结果显示, PmGRP94基因存在组织特异性, 并在肝胰腺中的表达量最高; 不同的应激条件下其表达具有明显差异, 其中在pH、铜(Cu)和锌(Zn)的应激下PmGRP94的表达显著升高。因此预测该基因可以作为斑节对虾受pH、Cu和Zn胁迫的指示基因。

Abstract: In the present study, the full-length of PmGRP94 was cloned from Penaeus monodon using RACE-PCR. The full-length of PmGRP94 cDNA was 2 990 bp containing a 5untranslated region (UTR) of 75 bp, a 3UTR of 527 bp and an open reading frame (ORF) of 2 388 bp. We analyzed the characterization and expression of tissue specificity of PmGRP94 when the shrimps were exposed with different pH values, salinities and three heavy metals using real time PCR (RT-PCR). The highest expression level was found in hepatopancreas. The transcription levels of PmGRP94 were significantly up-regulated under pH, copper and znic challenges. The results suggest that PmGRP94 might be a promising candidate marker for pH stress, cadmium and manganese exposure in shrimps.