王晓梅, 陈成勋, 邢克智, 王妍, 戴伟, 薛素青. 不同养殖密度下革胡子鲶幼鱼的生长和垂体GH基因mRNA表达分析[J]. 南方水产科学, 2015, 11(3): 35-40. DOI: 10.3969/j.issn.2095-0780.2015.03.006
引用本文: 王晓梅, 陈成勋, 邢克智, 王妍, 戴伟, 薛素青. 不同养殖密度下革胡子鲶幼鱼的生长和垂体GH基因mRNA表达分析[J]. 南方水产科学, 2015, 11(3): 35-40. DOI: 10.3969/j.issn.2095-0780.2015.03.006
WANG Xiaomei, CHEN Chengxun, XING Kezhi, WANG Yan, DAI Wei, XUE Suqing. Analysis of daily weight gain and GH mRNA expression in juvenile Clarias gariepinu reared at different stocking densities[J]. South China Fisheries Science, 2015, 11(3): 35-40. DOI: 10.3969/j.issn.2095-0780.2015.03.006
Citation: WANG Xiaomei, CHEN Chengxun, XING Kezhi, WANG Yan, DAI Wei, XUE Suqing. Analysis of daily weight gain and GH mRNA expression in juvenile Clarias gariepinu reared at different stocking densities[J]. South China Fisheries Science, 2015, 11(3): 35-40. DOI: 10.3969/j.issn.2095-0780.2015.03.006

不同养殖密度下革胡子鲶幼鱼的生长和垂体GH基因mRNA表达分析

Analysis of daily weight gain and GH mRNA expression in juvenile Clarias gariepinu reared at different stocking densities

  • 摘要: 革胡子鲶(Clarias gariepinus)幼鱼在35 km-3、65 kgm-3、95 kgm-3和125 kgm-3的密度下养殖60 d,第30和第60天时分析鱼的日增质量并应用半定量RT-PCR法分析垂体生长激素(GH)基因mRNA的相对表达量。0~30 d养殖期内,35 kgm-3和65 kgm-3密度组间及95 kgm-3和125 kgm-3密度组间的鱼日增质量差异不显著,但前2个密度组的鱼日增质量显著高于后2个密度组;而养殖第30天时各养殖密度组间GH基因mRNA相对表达量差异均不显著。30~60 d养殖期内,35 kgm-3和65 kgm-3密度组的日增质量显著高于125 kgm-3密度组,但其余各密度组间差异不显著;养殖第60天时,35 kgm-3和65 kgm-3 密度组GH基因mRNA相对表达量显著高于95 kgm-3和125 kgm-3密度组。结果表明革胡子鲶幼鱼的日增质量与垂体GH基因mRNA相对表达量不存在紧密的相关性。

     

    Abstract: Juvenile Clarias gariepinus were reared at densities of 35 kgm-3, 65 kgm-3, 95 kgm-3 and 125 kgm-3 for 60 d. On 30th and 60th day, we measured the daily weight gain (DWG) and assayed the mRNA level of GH in pituitary by semi-quantitative RT-PCR. During 0~30 d breeding, the DWGs showed no significant difference between 35 kgm-3and 65 kg m-3 treatments as well as between 95 kgm-3and 125 kg m-3 treatments, but the DWGs were significantly higher at 35 kgm-3 and 65 kgm-3 treatmentsthan those at 95 kgm-3 and 125 kgm-3 treatments. While on 30th day, significant difference was not found in the GH mRNA level of pituitary between any two stocking densities. During 30~60 d breeding, the DWGs of 35 kgm-3 and 65 kgm-3 treatments were significantly higher than that of 125 kgm-3 treatment; there was no significant difference in DWG between the other two treatments. On 60th day, the expressions of GH gene were significantly higher in 35 kgm-3 and 65 kgm-3 treatments than those in 95 kgm-3 and 125 kgm-3 treatments. The results indicate that there was no close correlation between DWG and mRNA level of GH in pituitary of juvenile C.gariepinus.

     

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