合浦珠母贝实时定量PCR内参基因的稳定性比较

Stability comparison of reference genes of Pinctada fucataby real-time qPCR

摘要: 以合浦珠母贝（Pinctada fucata）为研究对象，应用实时荧光定量PCR技术检测了甘油醛-3-磷酸脱氢酶（GAPDH）、-肌动蛋白（-actin）和18S核糖体rRNA（18S rRNA）3个管家基因在合浦珠母贝不同组织、性腺发育时期、胚胎发育时期mRNA水平的表达情况。同时比较了BestKeeper、geNorm和NormFinde软件对试验数据处理的差异，从中选出合适的内参基因。结果表明-actin在不同组织和胚胎发育不同阶段表达最稳定，18S rRNA在性腺发育不同时期表达最稳定。

Abstract: We analyze the expression stability of the housekeeping genes in Pinctada fucata, including Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH), beta-actin (-actin) and18S rRNA (18S), by quantitative real-time polymerase chain reaction (qRT-PCR) in different tissues, at different stages of gonadal development and embryonic development. Besides, we select the suitable reference genes by using softwares of BestKeeper, geNorm and NormFinder algorithms. The results indicate that -actin is the most stable reference gene for different tissues and different embryonic developmental stages, while 18S is the most stable reference gene for different gonadal developmental stages.