Abstract: We carried out the study to optimize the enrichment medium and culture conditions of Nocardia seriolea (ZJ0503). We studied the effects of temperature, salinity and initial pH on the growth of N.seriolea, investigated the carbon source, nitrogen source and inorganic ions of N.seriolea with single-factor experiment, and optimized different ingredients of medium with orthogonal experiment. The results indicate that the optimal growing condition of N.seriolea is temperature 25 ℃, salinity 5 and pH 6.50.2, the optimum carbon source is glucose, and nitrogen source yeast extract; that K2HPO4 and CaCl 2promote the growth of N.seriolea greatly. In addition, the optimum ingredients of medium are as follows: glucose 20 gL-1, yeast extract 15 gL-1, K2HPO4 0.75 gL-1, CaCl2 0.2 gL-1 (sterilized separately), and NaCl 5 gL-1, pH 6.50.2.