Abstract: We assessed the oosperm quality of turbot (Scophthalmus maximus) based on the morphological comparison of oosperm diameter and energy metabolism-related relative enzyme activities. The results show that the average oosperm diameter for turbot is (1.0530.020) mm and no significant difference is found among different batches. Bivariate correlation statistics reveal that the correlation is insignificant among oosperm diameter and suspensibility as well as fertilization rate and hatching rate. The statistics of energy metabolism-related relative enzyme activities indicate that significant correlation is found between hatching rate and aspartate amino transferase (AST) EAST(15.1593.300) Umg-1, HHatching rate＞20%, Spearman coefficient R=0.675, P=0.003; the correlation among suspensibility, fertilization rate and AST is significant. No significant correlation is found among pyruvate kinase (PK) , suspensibility, fertilization rate and hatching rate. Thus, the relative activity of AST can be used as a physiological and biochemical parameter to assess the oosperm quality of turbot, and protein is an important energetic source for the energy metabolism of turbots oosperm.