王信超, 孙敬敬, 范美华, 武梅, 石戈, 王日昕, 廖智. 厚壳贻贝血细胞颗粒的蛋白质组学分析[J]. 南方水产科学, 2012, 8(2): 7-14. DOI: 10.3969/j.issn.2095-0780.2012.02.002
引用本文: 王信超, 孙敬敬, 范美华, 武梅, 石戈, 王日昕, 廖智. 厚壳贻贝血细胞颗粒的蛋白质组学分析[J]. 南方水产科学, 2012, 8(2): 7-14. DOI: 10.3969/j.issn.2095-0780.2012.02.002
shu
WANG Xinchao, SUN Jingjing, FAN Meihua, WU Mei, SHI Ge, WANG Rixin, LIAO Zhi. Proteomic analysis of granule of hemocytes from Mytilus coruscus[J]. South China Fisheries Science, 2012, 8(2): 7-14. DOI: 10.3969/j.issn.2095-0780.2012.02.002
Citation: WANG Xinchao, SUN Jingjing, FAN Meihua, WU Mei, SHI Ge, WANG Rixin, LIAO Zhi. Proteomic analysis of granule of hemocytes from Mytilus coruscus[J]. South China Fisheries Science, 2012, 8(2): 7-14. DOI: 10.3969/j.issn.2095-0780.2012.02.002
shu

厚壳贻贝血细胞颗粒的蛋白质组学分析

Proteomic analysis of granule of hemocytes from Mytilus coruscus

    摘要
  • 摘要: 贝类的血细胞在宿主的免疫防御机制中发挥着重要的作用。文章利用线性离子阱-四级杆质谱对厚壳贻贝（Mytilus coruscus）血细胞的颗粒蛋白质组分进行了Shotgun分析，结合Mascot数据库搜索，共鉴定了125种高丰度蛋白，其中包括细胞骨架蛋白、细胞粘附蛋白、精氨酸激酶和代谢相关酶类等。此结果为深入了解厚壳贻贝血细胞颗粒的蛋白组成、贻贝免疫分子标记的筛选及其免疫机制奠定了基础。

     

    Abstract: The blood cells of mussel play an important role in the hosts immune defense mechanism. We explore the protein composition in the granule of hemocytes from Mytilus coruscus by using linear-lon-trap-quadrupole mass spectrometry (LTQ-Orbitrap) combined with Shotgun strategy. A total of 125 high-abundance proteins are identified by Mascot searching methods, including cytoskeletal proteins, cell adhesion proteins, arginine kinases and metabolism-related enzymes, etc.. The results lay a foundation for further studies on the composition of mussel protein particles, screening of its immune molecular markers as well as its immune mechanism.

     

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