卵形鲳鲹碱性磷酸酶和酸性磷酸酶的分布及其低温保存

区又君; 罗奇; 李加儿

doi:10.3969/j.issn.2095-0780.2011.02.008

卵形鲳鲹碱性磷酸酶和酸性磷酸酶的分布及其低温保存

Distribution and cryopreservation of alkaline phosphatase(AKP) and acid phosphatase(ACP) in Trachinotus ovatus

摘要

摘要: 测定了卵形鲳鲹（Trachinotus ovatus）的碱性磷酸酶（AKP）和酸性磷酸酶（ACP）在不同器官和肌肉中的活性；将样品分别置于4 ℃、－20 ℃和－80 ℃下保存3 d、7 d、15 d和30 d，研究不同保存温度和保存时间对样品中AKP和ACP酶活性的影响。结果表明，在卵形鲳鲹胃中未检测到AKP活性，其他器官中均检测到AKP活性，AKP活性大小顺序依次为后肠＞幽门盲囊＞肾＞中肠＞肝＞前肠＞心＞肌肉；ACP在卵形鲳鲹体内分布较广，所测器官中均有ACP活性，其活性大小顺序为肝＞肌肉＞后肠＞中肠＞幽门盲囊＞肾＞心＞前肠＞胃。低温保存试验结果表明，AKP和ACP在4 ℃和－20 ℃下保存，时间越长，活力越低；在－80 ℃下保存，AKP和ACP活力在30 d内基本稳定，只有幽门盲囊的AKP和ACP在长时间保存后活力明显下降。

Abstract: The distribution and cryopreservation of alkaline phosphatase (AKP) and acid phosphatase (ACP) activities in Trachinotus ovatus were studied. The samples were keptat 4 ℃, -20 ℃and -80 ℃ for 3 d, 7 d, 15 dand 30 d, respectively, to investigate the effect of different storage temperatures and time on AKP and ACP activities. The results show that AKP activity has been tested in different organs except stomach, and the order of AKP activity is: hindgut＞pyloric caecum＞kidney＞midgut＞liver＞foregut＞heart＞muscle. ACP has been tested in different organs, and the order of ACP activity is: liver＞muscle＞hindgut＞midgut＞pyloric caecum＞kidney＞heart＞foregut＞stomach. The result of cryopreservation experiment indicates that both AKP and ACP activities obviously weaken as the storage time prolongs at 4℃ and -20 ℃, but remain steady at -80 ℃ within 30 d except for pyloric caecum.

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