赵永强, 杨贤庆, 戚勃, 李来好, 刁石强, 陈胜军. 琼胶中硫酸基的离子色谱法测定[J]. 南方水产科学, 2009, 5(6): 8-12. DOI: 10.3969/j.issn.1673-2227.2009.06.002
引用本文: 赵永强, 杨贤庆, 戚勃, 李来好, 刁石强, 陈胜军. 琼胶中硫酸基的离子色谱法测定[J]. 南方水产科学, 2009, 5(6): 8-12. DOI: 10.3969/j.issn.1673-2227.2009.06.002
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ZHAO Yongqiang, YANG Xianqing, QI Bo, LI Laihao, DIAO Shiqiang, CHEN Shengjun. Determination of sulfate group in agar by ion chromatography[J]. South China Fisheries Science, 2009, 5(6): 8-12. DOI: 10.3969/j.issn.1673-2227.2009.06.002
Citation: ZHAO Yongqiang, YANG Xianqing, QI Bo, LI Laihao, DIAO Shiqiang, CHEN Shengjun. Determination of sulfate group in agar by ion chromatography[J]. South China Fisheries Science, 2009, 5(6): 8-12. DOI: 10.3969/j.issn.1673-2227.2009.06.002
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琼胶中硫酸基的离子色谱法测定

Determination of sulfate group in agar by ion chromatography

    摘要
  • 摘要: 确立了离子色谱法检测琼胶中硫酸基含量的样品处理方法和检测条件。样品前处理采用灰化降解琼胶粉中的有机成分，超纯水溶解定容、过膜后测定；检测时利用戴安ICS-3000型离子色谱仪、电导检测器、ASRS 4 mm抑制器和IonPacAS23型分离柱，淋洗液组成为9.0 mmolL-1 Na2CO3溶液和1.6 mmolL-1 NaHCO3溶液，流速1.0 mLmin-1。此方法硫酸根 (SO42-) 在1.0~15.0 mgL-1浓度范围内呈良好的线性关系（R2=0.9995），检出限为0.01 mgL-1，样品加标回收率为89.0%~102.0%，分析快速准确。离子色谱法与传统方法相比，操作快速简便，灵敏度高，重现性好。

     

    Abstract: An ion chromatography method for the determination of sulfate content in agar was established. After being degraded the organic components of agar via ashing, the samples were dissolved in ultrapure water, then filtrated by a membrane of 0.45 m. The sulfate was detected by DIONEX ICS-3000 ion chromatography equipped with a conductivity detector, ASRS 4 mm suppressor and IonPac AS23 column, using 9.0 mmolL-1 Na2CO3 and 1.6 mmolL-1 NaHCO3 as eluents. The flow rate of eluent was 1.0 mLmin-1. The results showed that the detection of sulfate had good linearity within concentration range of 1.0 mgL-1 to 15.0 mgL-1 (R2=0.9995). The detection limit of this method was 0.1 mgL-1, andthe recoveries of spiked matrix ranged from 89% to 102%. Compared with the traditional methods, ion chromatography was simple, rapid, accurate and sensitive.

     

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