凝胶渗透色谱-气相-质谱测定鲸皮脂中的多氯联苯

Determination of residual polychlorinated biphenyls (PCBs) in blubble of whale by gel permeation chromatography and gas chromatography/mass spectrometry

摘要: 建立了一种测定鲸鱼皮脂中多氯联苯（PCBs）前处理的改良方法，即将皮脂样品分别经超声波萃取、GPC净化和浓缩等处理后，用GC-MS测定。结果显示，该方法加标回收率为73%~118%，精密度为4.1%~9.7%，检出限在0.19~1.07 ngg-1之间。该方法操作简单，净化效果好、准确性高、重复性强，适合鲸鱼皮脂中PCBs含量的分析测定。

Abstract: An ameliorated pretreatment method for determination of polychlorinated biphenyls（PCBs）in blubber of whale has been established. The samples were extracted with ethyl acetate-cyclohexane by ultrasonically assisted procedure. The extract was purified and condensed with GPC and PCBs were determined by GC-MS. The results showed that the recoveries of spiked matrix were between 73% and 118%, the coefficient of variation was 4.1%~9.7%. The detection limits of the method were 0.19~1.07 ngg-1. This method，with easy manipulation, well purification, and good accuracy and repetitiveness, is suitable to determine the contents of the PCBs in the whale.