陈亮, 张殿昌, 杨丽诗, 黄建华, 杨其彬, 江世贵. 斑节对虾真核生物翻译起始因子3亚基4(btseIF3g)序列分析及其组织表达特性[J]. 南方水产科学, 2009, 5(3): 1-9. DOI: 10.3969/j.issn.1673-2227.2009.03.001
引用本文: 陈亮, 张殿昌, 杨丽诗, 黄建华, 杨其彬, 江世贵. 斑节对虾真核生物翻译起始因子3亚基4(btseIF3g)序列分析及其组织表达特性[J]. 南方水产科学, 2009, 5(3): 1-9. DOI: 10.3969/j.issn.1673-2227.2009.03.001
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CHEN Liang, ZHANG Dianchang, YANG Lishi, HUANG Jianhua, YANG Qibin, JIANG Shigui. Sequence analysis of eukaryotic translation initiation factor 3 subunit G (eIF3g) of black tiger shrimp (Penaeus monodon) and its mRNA tissue expression[J]. South China Fisheries Science, 2009, 5(3): 1-9. DOI: 10.3969/j.issn.1673-2227.2009.03.001
Citation: CHEN Liang, ZHANG Dianchang, YANG Lishi, HUANG Jianhua, YANG Qibin, JIANG Shigui. Sequence analysis of eukaryotic translation initiation factor 3 subunit G (eIF3g) of black tiger shrimp (Penaeus monodon) and its mRNA tissue expression[J]. South China Fisheries Science, 2009, 5(3): 1-9. DOI: 10.3969/j.issn.1673-2227.2009.03.001
shu

斑节对虾真核生物翻译起始因子3亚基4(btseIF3g)序列分析及其组织表达特性

Sequence analysis of eukaryotic translation initiation factor 3 subunit G (eIF3g) of black tiger shrimp (Penaeus monodon) and its mRNA tissue expression

    摘要
  • 摘要: 通过mRNA差异显示（differential display，DD）技术筛选的差异片段，与斑节对虾（Penaeus monodon）混合组织cDNA文库中的EST进行拼接，获得斑节对虾真核生物翻译起始因子3亚基4（btseIF3g）的全序列（GenBank登录号：FJ829364）。btseIF3g全长1 041 bp，由72 bp 5非编码区、90 bp 3非编码区以及879 bp的开放阅读框组成，推测编码一个长度为292个氨基酸的蛋白。经BLAST分析，btseIF3g C端含有一个RNA识别基序（RNA recognition motif，RRM）的保守结构域（E值为2.72e-25）。利用氨基酸序列进行系统发育分析表明，btseIF3g与eIF3g属于同一分支。采用荧光定量PCR（Real-time PCR）方法对250日龄的雌、雄斑节对虾不同组织表达特性研究发现，btseIF3g在眼柄神经节、脑神经、肝胰腺、性腺和肌肉组织中均有表达，在卵巢中的相对表达量极显著（P0.01）高于其他组织，这一结果提示eIF3g可能与斑节对虾卵巢发育相关。

     

    Abstract: In order to analyze the function of a differential expression cDNA fragment of gonads of domesticated broodstock black tiger shrimp (Penaeus monodon), the black tiger shrimp eukaryotic translation initiation factor 3 subunit G (btseIF3g) had been found by spliced with the EST, after screening in the P.monodon mix cDNA library. The full length cDNA of btseIF3g (GenBank accession no. FJ829364) contained a 5untranslated region (UTR) of 72 bp, a 3UTR of 90 bp and an ORF of 879 bp encoding a polypeptide of 292 amino acids with an estimated molecular mass of 32.97 kDa. RNA recognition motif (RRM) was found in the btseIF3g deduced amino acid sequence. Phylogenetic analysis of the eIF3 gene family indicated that the btseIF3g was homologous to the eIF3g. mRNA expression of btseIF3g could be detected in 250-day-old P.monodons eyestalk, brain, hepatopancreas, gonad and muscle by fluorescent quantitative real-time PCR analysis. The highest expression level of btseIF3g in the ovary indicated that btseIF3g played an important role in oogenesis.

     

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