乔娣, 雷宁, 朱俊杰, 张超楠, 王艳超, 周玲. 大口黑鲈幼鱼肝脏抗大口黑鲈弹状病毒应答的转录组分析[J]. 南方水产科学. DOI: 10.12131/20240050
引用本文: 乔娣, 雷宁, 朱俊杰, 张超楠, 王艳超, 周玲. 大口黑鲈幼鱼肝脏抗大口黑鲈弹状病毒应答的转录组分析[J]. 南方水产科学. DOI: 10.12131/20240050
QIAO Di, LEI Ning, ZHU Junjie, ZHANG Chaonan, WANG Yanchao, ZHOU Ling. Transcriptome analysis of liver anti-MSRV responses in juvenile largemouth bass (Micropterus salmoides)[J]. South China Fisheries Science. DOI: 10.12131/20240050
Citation: QIAO Di, LEI Ning, ZHU Junjie, ZHANG Chaonan, WANG Yanchao, ZHOU Ling. Transcriptome analysis of liver anti-MSRV responses in juvenile largemouth bass (Micropterus salmoides)[J]. South China Fisheries Science. DOI: 10.12131/20240050

大口黑鲈幼鱼肝脏抗大口黑鲈弹状病毒应答的转录组分析

Transcriptome analysis of liver anti-MSRV responses in juvenile largemouth bass (Micropterus salmoides)

  • 摘要: 为探究大口黑鲈 (Micropterus salmoides) 对大口黑鲈弹状病毒 (Micropterus salmoides rhabdovirus, MSRV) 的抗病反应和代谢调控网络,揭示其抗病的免疫分子机制并为后续大口黑鲈的分子生物学研究提供基础数据,利用Illumina NovaSeq6000测序平台,对MSRV感染的大口黑鲈易感组、抗病组和对照组的肝脏组织进行转录组测序分析,对所得基因的功能注释发现,被注释的差异基因主要与细胞过程、细胞、结合和催化活性等功能有关。KEGG通路富集分析结果显示,大口黑鲈肝脏组织在MSRV感染下高表达的差异基因富集在药物代谢-细胞色素P450、细胞色素P450对外源性药物的代谢作用、蛋白酶体、抗坏血酸和醛酸盐代谢、脂肪酸降解等代谢相关通路;进一步筛选免疫相关基因进行通路分析发现,与抗MSRV免疫应答相关的通路主要为NOD样受体信号传导、C型凝集素受体信号、细胞溶质DNA传感途径、Toll样受体信号传导和RIG-I样受体信号传导等。最后,通过qRT-PCR验证了差异基因的变化趋势与转录组测序分析结果的一致性,证明了转录组数据的可靠性。获得的差异基因和调控通路可为大口黑鲈抗MSRV免疫的分子机制和疾病防控研究提供理论基础。

     

    Abstract: In order to investigate the disease resistance and metabolic regulatory network of Micropterus salmoides to M. salmoides rhabdovirus, uncover the immunomolecular mechanism of its disease resistance, and provide genetic data references for subsequent molecular biology investigation of M. salmoides, we used the Illumina NovaSeq 6000 sequencing platform to analyze the transcriptome sequencing of liver tissues from susceptible group, disease-resistant group and control group of M. salmoides infected with M. salmoides rhabdovirus. Functional annotation of obtained genes reveals that the annotated differentially expressed genes were mainly associated with functions such as cellular process, cell, binding and catalytic activity, etc. The KEGG pathway enrichment analysis indicates that the differentially expressed genes with high expression levels in M. salmoides liver tissue with M. salmoides rhabdovirus infection were enriched in metabolic pathways, including drug metabolism-cytochrome P450, metabolism of xenobiotics by cytochrome P450, proteasome, ascorbate and aldarate metabolism, fatty acid degradation, as well as other metabolic processes. Further screening of immune-related genes for pathway analysis shows that the main pathways associated with the immune response against M. salmoides rhabdovirus were NOD-like receptor signaling pathway, C-type lectireceptor signaling pathway, cytosolic DNA-sensing pathway, Toll-like receptor signaling pathway, RIG-I-like receptor signaling pathway, etc. Finally, we verified the consistency of the differential gene trends with the results of transcriptome sequencing analysis by qRT-PCR, demonstrating the reliability of the transcriptome data. The differential genes and regulatory pathways identified in this study will provide a theoretical basis for research on the molecular mechanism of M. salmoides immunity against M. salmoides rhabdovirus as well as disease prevention and control.

     

/

返回文章
返回