郑秋实, 刘永, 李纯厚, 吴鹏, 肖雅元, 林琳, 刘玉, 邹剑. 季节变化对珠江口棘头梅童鱼肠道和水体中细菌群落结构的影响[J]. 南方水产科学. DOI: 10.12131/20240030
引用本文: 郑秋实, 刘永, 李纯厚, 吴鹏, 肖雅元, 林琳, 刘玉, 邹剑. 季节变化对珠江口棘头梅童鱼肠道和水体中细菌群落结构的影响[J]. 南方水产科学. DOI: 10.12131/20240030
ZHENG Qiushi, LIU Yong, LI Chunhou, WU Peng, XIAO Yayuan, LIN Lin, LIU Yu, ZOU Jian. Seasonal effects on bacterial community between intestine of Collichthys lucidus and water environment from Pearl River Estuary[J]. South China Fisheries Science. DOI: 10.12131/20240030
Citation: ZHENG Qiushi, LIU Yong, LI Chunhou, WU Peng, XIAO Yayuan, LIN Lin, LIU Yu, ZOU Jian. Seasonal effects on bacterial community between intestine of Collichthys lucidus and water environment from Pearl River Estuary[J]. South China Fisheries Science. DOI: 10.12131/20240030

季节变化对珠江口棘头梅童鱼肠道和水体中细菌群落结构的影响

Seasonal effects on bacterial community between intestine of Collichthys lucidus and water environment from Pearl River Estuary

  • 摘要: 棘头梅童鱼 (Collichthys lucidus) 是珠江口常见的优势种和经济鱼类物种。为揭示棘头梅童鱼的生物生态学特征,利用16S rRNA基因的高通量测序技术,探究了季节变化对珠江口棘头梅童鱼肠道和水体中细菌群落结构的影响。结果表明,鱼类肠道细菌以变形菌门居多 (78.89%),其次为厚壁菌门 (6.70%) 和拟杆菌门 (5.45%);水体细菌以变形菌门 (40.32%) 和拟杆菌门 (37.97%) 为主。棘头梅童鱼肠道中的优势菌属存在明显季节变化,春、夏季鱼类肠道中优势细菌为嗜冷杆菌属 (Psychrobacter),秋季为发光杆菌属 (Photobacterium),而冬季为假单胞菌属 (Pseudomonas)、Sva0996 marine group、弧菌属 (Vibrio)和甲基杆菌 (Methylobacterium)。冬季棘头梅童鱼肠道菌群的α多样性指数最高,秋季最低。春季和夏季鱼类肠道细菌组成无显著性差异 (P>0.05),而其余季节间存在显著性差异 (P<0.05)。水体细菌群落组成的季节变化与鱼类肠道菌群变化相似。水环境中TenacibaculumPsychrobacter、Candidatus_ActinomarinaSalinimonas和OM60NOR5_clade共5个菌属与鱼类肠道细菌组成之间存在显著相关性 (R>0.4, P<0.01)。细菌共现网络分析显示冬季鱼类肠道和水环境的共有菌群比例最低,而夏季最高。研究表明,季节变化对珠江口棘头梅童鱼的肠道菌群和水体细菌群落组成均存在影响。

     

    Abstract: Collichthys lucidus is a common dominant and economically important fish species in the Pearl River Estuary. To reveal the ecological characteristics of C. lucidus, we applied high-throughput sequencing technology of 16S rRNA gene to investigate the effects of seasonal changes on the bacterial community structure in the intestines and water body of the Pearl River Estuary. The bacterial community in the fish intestines was predominantly composed of members of Proteobacteria (78.89%), followed by Firmicutes (6.70%) and Bacteroidota (5.45%), while Proteobacteria (40.32%) and Bacteroidota (37.97%) dominated the surrounding water. Besides, we observed the seasonal changes in the dominant bacterial genera in the intestine of C. lucidus. The relative abundance of Psychrobacter was significantly higher in spring and summer, whereas Photobacterium predominated in autumn. However, Pseudomonas, Sva0996 marine group, Vibrio and Methylobacterium were more abundant in winter. The α-diversity index values of the intestinal bacteria of C. lucidus were highest in winter but lowest in autumn. PCoA and ANOSIM analyses reveal no significant differences in the intestinal bacterial composition between spring and summer (P>0.05), whereas significant differences were observed in the other seasons (P<0.05). The seasonal changes in the bacterial composition of surrounding water were similar to those in the fish intestinal bacteria. Mantel test analysis finds out that five bacterial genera (Tenacibaculum, Psychrobacter, Candidatus_Actinomarina, Salinimonas, OM60NOR5_clade) in water environment showed a significantly positive correlation with the intestinal bacteria of C. lucidus (R>0.4, P<0.01). Bacterial co-occurrence network analysis shows that the proportion of shared bacterial communities between the fish intestines and the water environment was lowest in winter but highest in summer. The results indicate that seasonal changes can impact both the intestinal bacterial communities of C. lucidus from the Pearl River Estuary and bacterial communities in water body.

     

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