徐静雯, 钟立强, 张世勇, 刘洪岩, 陈校辉, 王明华. 施肥对斑点叉尾鮰肠道菌群的影响[J]. 南方水产科学. DOI: 10.12131/20230240
引用本文: 徐静雯, 钟立强, 张世勇, 刘洪岩, 陈校辉, 王明华. 施肥对斑点叉尾鮰肠道菌群的影响[J]. 南方水产科学. DOI: 10.12131/20230240
XU Jingwen, ZHONG Liqiang, ZHANG Shiyong, LIU Hongyan, CHEN Xiaohui, WANG Minghua. Effect of fertilization on intestinal microbiota of Ictalurus punctatus[J]. South China Fisheries Science. DOI: 10.12131/20230240
Citation: XU Jingwen, ZHONG Liqiang, ZHANG Shiyong, LIU Hongyan, CHEN Xiaohui, WANG Minghua. Effect of fertilization on intestinal microbiota of Ictalurus punctatus[J]. South China Fisheries Science. DOI: 10.12131/20230240

施肥对斑点叉尾鮰肠道菌群的影响

Effect of fertilization on intestinal microbiota of Ictalurus punctatus

  • 摘要: 为探索斑点叉尾鮰 (Ictalurus punctatus) 的绿色、经济养殖模式,并为其养殖过程的施肥管理提供指导,研究了施肥对斑点叉尾鮰肠道菌群的影响。将斑点叉尾鮰分为有机肥组 (Y)、无机肥组 (W) 与对照组 (D) 共3 组,进行为期3个月的养殖实验。实验结束后,取出肠道内容物,通过16S rRNA 扩增子测序技术检测肠道菌群。结果显示,施肥后斑点叉尾鮰肠道菌群的多样性与丰度均有所降低。在门水平上,3组的优势菌群均为厚壁菌门、变形菌门和拟杆菌门,对照组中的变形菌门、放线菌门与梭杆菌门的相对丰度显著高于施肥组 (p<0.05);在属水平上,与对照组相比,施肥组中以罗姆布茨菌属 (Romboutsia) 和苏黎世杆菌属 (Turicibacter) 为优势菌属;其中,无机肥组中链球菌属 (Streptococcus) 相对丰度显著上升 (p<0.05),对照组中的梭杆菌属 (Fusobacterium) 相对丰度高于施肥组 (p<0.05)。经3组菌群差异性分析,无机肥组鱼体肠道中的乳酸菌含量显著增加,有机肥组中的链球菌属显著下调,降低了斑点叉尾鮰的发病机率。研究表明,在斑点叉尾鮰养殖中混合施加有机与无机肥料,能有效促进其健康生长。

     

    Abstract: In order to explore a green and economical breeding model for Ictalurus punctatus and provide guidance for fertilization management in its breeding process, we investigated the effect of fertilization on its gut microbiota. We designed a three-month experiment and divided the fish into three groups: organic fertilizer group (Y), inorganic fertilizer group (W) and control group (D). The intestinal contents of fish were collected and the intestinal microbiota was detected by 16S rRNA amplification technique. The results show that the diversity and abundance of intestinal microbiota decreased after fertilization. On phylum level, Firmicutes, Proteobacteria and Bacteroidetes were the dominant bacterial phyla. The relative abundance of Proteobacteria, Actinobacteria and Fusobacteriota in the control group was significantly higher than that in the fertilization group. On genus level, Romboutsia and Turicibacter were the dominant bacteria in the fertilization groups compared with the control group. The relative abundance of Streptococcus in the inorganic fertilizer group increased significantly (p<0.05). The relative abundance of Fusobacterium in the control group was higher than that in the inorganic fertilizer group (p<0.05). According to the difference analysis of the flora among the three groups, the content of lactic acid bacteria in the intestinal tract of fish in the inorganic fertilizer group increased significantly, while Streptococcus in the organic fertilizer group was significantly downregulated, reducing the incidence of catfish disease. The results show that the application of organic and inorganic fertilizer in aquaculture can promote the healthy growth of fish effectively.

     

/

返回文章
返回