余坦健, 简纪常, 林兴, 简贺君, 黄郁葱. 十二烷基苯磺酸钠对奥尼罗非鱼免疫毒性的研究[J]. 南方水产科学, 2008, 4(3): 33-37.
引用本文: 余坦健, 简纪常, 林兴, 简贺君, 黄郁葱. 十二烷基苯磺酸钠对奥尼罗非鱼免疫毒性的研究[J]. 南方水产科学, 2008, 4(3): 33-37.
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YU Tanjian, JIAN Jichang, LIN Xing, JIAN Hejun, HUANG Yucong. Studies on immunotoxicity of phenol and SDBS to hybrid tilapia (Tilapia niloticaT.aurea)[J]. South China Fisheries Science, 2008, 4(3): 33-37.
Citation: YU Tanjian, JIAN Jichang, LIN Xing, JIAN Hejun, HUANG Yucong. Studies on immunotoxicity of phenol and SDBS to hybrid tilapia (Tilapia niloticaT.aurea)[J]. South China Fisheries Science, 2008, 4(3): 33-37.
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十二烷基苯磺酸钠对奥尼罗非鱼免疫毒性的研究

Studies on immunotoxicity of phenol and SDBS to hybrid tilapia (Tilapia niloticaT.aurea)

    摘要
  • 摘要: 用5种浓度的十二烷基苯磺酸钠（sodium dodecyl benzene sulfonic，SDBS）浸泡刺激奥尼罗非鱼（Tilapia nilotica ♀T.aurea ♂）8周，每2周采血样1次，测定免疫指标。0.05和0.10 mgL-1组对奥尼罗非鱼的氯化硝基四氮唑兰（nitro blue tetrazolium，NBT）阳性细胞和IgM含量无影响。0.40 mgL-1组在第8周时，IgM含量显著下降，而NBT阳性细胞突然急剧下降，与对照组相比差异极显著。0.70 mgL-1组从第4周起，NBT阳性细胞和IgM含量开始减少，第6周时差异显著。1.00 mgL-1组NBT阳性细胞数在第2周时量就已经有减少，但与对照组相比差异不显著，到第4周时差异极其显著；在第4周时IgM含量有显著差异，第6周后差异极显著。攻毒试验表明，0.40、0.70和1.00 mgL-1组的死亡率均高于对照组。以上结果表明，当SDBS浓度大于0.40 mgL-1时，奥尼罗非鱼免疫功能不同程度受到抑制，对NBT阳性细胞和IgM的影响存在着时间与剂量效应。

     

    Abstract: The main immune indexes of hybrid tilapia (Tilapia nilotica♀T.aurea♂) were measured every two weeks. The 0.05 and 0.10 mgL-1 sodium dodecyl benzene sulfonic (SDBS) had not affected the numbers of NBT-positive cells and content of IgM. The 0.40 mgL-1 SDBS significantly reduced the numbers of NBT-positive cells and content of IgM in eighth week. The 0.70 mgL-1 SDBS significantly reduced the numbers of NBT-positive cells and content of IgM in sixth week. The 0.10 mgL-1 SDBS significantly reduced the numbers of NBT-positive cells and content of IgM in fourth week. Mortality of hybrid tilapia after injection of Aeromonas hydrophila (0.40, 0.70 and 1.00 mgL-1 SDBS) was higher than the control. These results indicated that low concentration of SDBS (0.05 and 0.10 mgL-1) did not affect the immune function of hybrid tilapia, but the higher concentrations of SDBS (0.40, 0.70 and 1.00 mgL-1) will apparently reduce the immune function of hybrid tilapia along with time changed.

     

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