十二烷基苯磺酸钠对奥尼罗非鱼免疫毒性的研究

余坦健; 简纪常; 林兴; 简贺君; 黄郁葱

十二烷基苯磺酸钠对奥尼罗非鱼免疫毒性的研究

1.
广东海洋大学水产学院，广东省水产经济动物病原生物学及流行病学重点实验室暨广东省教育厅水产经济动物病害控制重点实验室，广东湛江 524025；
2.
福建省康地饲料开发有限公司，福建福州 350007

详细信息

作者简介:
余坦健（1980-），男，硕士，助理工程师，从事水产经济动物免疫与饲料研究。E-mail:ytjian1980@163.com

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出版历程
- 收稿日期: 2008-01-30
- 修回日期: 2008-03-21
- 刊出日期: 2008-06-05

Studies on immunotoxicity of phenol and SDBS to hybrid tilapia (Tilapia niloticaT.aurea)

1.
Fisheries College of Guangdong Ocean University, Guangdong Province Key Lab. of Pathogen Biology and Epidemiology of Aquatic Economic Animals Education Department Key Lab. of Control for Diseases of Aquatic Economic Animals, Zhanjiang 524025, China;
2.
Fujian Kangdi Feed Development Limited Company, Fuzhou 350007, China

摘要

摘要: 用5种浓度的十二烷基苯磺酸钠（sodium dodecyl benzene sulfonic，SDBS）浸泡刺激奥尼罗非鱼（Tilapia nilotica ♀T.aurea ♂）8周，每2周采血样1次，测定免疫指标。0.05和0.10 mgL-1组对奥尼罗非鱼的氯化硝基四氮唑兰（nitro blue tetrazolium，NBT）阳性细胞和IgM含量无影响。0.40 mgL-1组在第8周时，IgM含量显著下降，而NBT阳性细胞突然急剧下降，与对照组相比差异极显著。0.70 mgL-1组从第4周起，NBT阳性细胞和IgM含量开始减少，第6周时差异显著。1.00 mgL-1组NBT阳性细胞数在第2周时量就已经有减少，但与对照组相比差异不显著，到第4周时差异极其显著；在第4周时IgM含量有显著差异，第6周后差异极显著。攻毒试验表明，0.40、0.70和1.00 mgL-1组的死亡率均高于对照组。以上结果表明，当SDBS浓度大于0.40 mgL-1时，奥尼罗非鱼免疫功能不同程度受到抑制，对NBT阳性细胞和IgM的影响存在着时间与剂量效应。
- 十二烷基苯磺酸钠（SDBS） /
- 奥尼罗非鱼 /
- 免疫指标 /
- NBT阳性细胞 /
- IgM
Abstract: The main immune indexes of hybrid tilapia (Tilapia nilotica♀T.aurea♂) were measured every two weeks. The 0.05 and 0.10 mgL-1 sodium dodecyl benzene sulfonic (SDBS) had not affected the numbers of NBT-positive cells and content of IgM. The 0.40 mgL-1 SDBS significantly reduced the numbers of NBT-positive cells and content of IgM in eighth week. The 0.70 mgL-1 SDBS significantly reduced the numbers of NBT-positive cells and content of IgM in sixth week. The 0.10 mgL-1 SDBS significantly reduced the numbers of NBT-positive cells and content of IgM in fourth week. Mortality of hybrid tilapia after injection of Aeromonas hydrophila (0.40, 0.70 and 1.00 mgL-1 SDBS) was higher than the control. These results indicated that low concentration of SDBS (0.05 and 0.10 mgL-1) did not affect the immune function of hybrid tilapia, but the higher concentrations of SDBS (0.40, 0.70 and 1.00 mgL-1) will apparently reduce the immune function of hybrid tilapia along with time changed.
- sodium dodecy benzene sulfonic (SDBS) /
- hybrid tilapia /
- immune index /
- NBT-positive cell /
- IgM