Abstract: To investigate the effect of acute temperature stress on biochemical indicators of juvenile Pampus argenteus, we designed two treatment groups (22 ℃ and 32 ℃) and a control group (27 ℃) with stress time of 42 h. The GPT in serum and GOT in liver in two experimental groups changed oppositely; AKP varied in an opposite manner but ACP changed in the same way; LDH in 22 ℃ group changed in a wave-like manner (P < 0.05).Na⁺, K⁺, Cl^- and Ca^{2 +} concentrations in three groups changed differently. The Na⁺/K⁺-ATPase activity in gill in 32 ℃ group decreased, but that in kidney in 22 ℃ group increased first then decreased (P < 0.05).In two experimental groups, Ca²⁺/Mg²⁺-ATPase activities in gill and kidney varied in oppositely (P < 0.05). It is suggested that acute temperature stress, which should be avoided in breeding for juvenile P.argenteus, affects normal metabolism of the juveniles significantly.