Abstract: To investigate the effect of nutrient addition on germination, growth and survival of Gloiopeltis furcata discs as well as the influence on growth and biochemical composition of G.furcata thalli, we incubated the discs and thalli of G. furcata with different concentrations of nutrient (adding NaNO₃ of 0 mg·L^-1, 10 mg·L^-1, 40 mg·L^-1, 80 mg·L^-1, 10 mg·L^-1, 40 mg·L^-1, 80 mg·L^-1and corresponding K₂HPO₄ of 0 mg·L^-1, 0 mg·L^-1, 0 mg·L^-1, 0 mg·L^-1, 1 mg·L^-1, 4 mg·L^-1, 8 mg·L^-1). No significant difference was found in sprouting and survival rate of discs as well as the weight of thalli between groups added with nutritional salt and the control, but the contents of chlorophyll and phycobiliprotein of thalli in groups added with NaNO₃ of 40~80 mg·L^-1 were significantly higher than that in the control (P < 0.05).It is showed that it needs not add nutritional salt for incubation of G.furcata discs but it needs add NaNO₃ of 10~40 mg·L^-1and K₂HPO₄ of 4 mg·L^-1for incubation of G.furcata thalli.