Abstract: We randomly assigned 360 juvenile yellow catfish(Peltobagrus fulvidraco) with average body weight 2.48 g to 4 groups, and fed the control (G0) with basal diet, the other 3 groups (G100, G200 and G400) with basal diet supplemented with 100 mg·kg^-1, 200 mg·kg^-1 and 400 mg·kg^-1 complete enzyme preparation (CEP), respectively. After feeding for 8 weeks, the weight gain rate (WGR), specific growth rate (SGR) and feed intake (FI) increased with the CEP concentration. Besides, the WGR, SGR and FI of G400 were higher than those of the control by 15.4%, 7.5% and 20.3%(P < 0.05), respectively. No significant difference was found among all the treatments in body moisture, crude protein, crude lipid and ash content (P > 0.05). Serum urea content decreased with increasing CEP in diets, while glutamic-pyruvic transaminase (ALT), glutamic-oxalacetic transaminase (AST) activities and total protein(TP)content increased (P > 0.05). The activity of lysozyme(LSZ)increased and then decreased(P < 0.05). In conclusion, the feed added with 400 mg·kg^-1 CEP can promote the growth and ALT activities of yellow catfish, and decrease its urea content; the feed added with 200 mg·kg^-1CEP can significantly increase the TP content and LSZ and AST activities of the fish.