Abstract: Lots of studies have proved that Kiss gene and its receptor gene Kissr play an important role in the regulation of the gonadotropic axis, especially in the timing of puberty in vertebrates. We identified the gonad development of blunt snout bream (Megalobrama amblycephala) from stage Ⅰ to stage Ⅵ using tissue slice technology, detected the Kiss2 and Kiss2r gene transcription in the brain through quantitative real time PCR (qPCR), and collected pituitary and gonad tissues of the samples during different stages. The qPCR results show that Kiss2/Kiss2r play roles in the onset of puberty in M.amblycephala by increasing the expression of pituitary and gonad during ovary development in stage Ⅱ, and the expression of testis in stage Ⅲ.