Abstract: We investigated the effects of Ca^{2 +} concentrations on element content of fish body and otolith of juvenile Paralichthys olivaceus that were raised at 20℃ in artificial seawater for 60 more days. Five groups of seawater with Ca^{2 +} concentrations 200 mg·L^-1 (group A), 400 mg·L^-1 (group B, control), 800 mg·L^-1 (group C), 1 600 mg·L^-1 (group D), and 3 000 mg·L^-1 (group E), were used for raise. It was found that with the increase of Ca²⁺ concentration, the contents of P, Na, Mg, Sr, Fe, Mn and Ni in fish body decreased, and that Ca²⁺ concentrations significantly influenced the otolith length, width, L/W and weight. Specifically, otolith length and width of juvenile P.olivaceus group A were significantly lower than that of group B and C; L/W was significantly lower than that of group D; and otolith weight was significantly lower than that of group B and D. Sr content of otolith, significantly, and the ratio of Sr/Ca and Mg/Ca also decreased with increasing concentration of Ca²⁺. The results indicate that Ca²⁺ concentrations affect juvenile P.olivaceus significantly in terms of its body and otolith element content formation, composition and accumulation.