Abstract: Juvenile seabream (Chrysophrys major) at body length of 4.4~6.6 cm in South China Sea was acoustic tamed continuously for 8 d by rectangular continuant at 500 Hz under laboratory conditions, and the data was analyzed with Gaussion model (Origin software). The results show that the fish in the control distribute randomly. The distance of the trial groups is 105.40 cm, 93.51 cm, 88.04 cm, 83.40 cm, 71.86 cm, 83.06 cm, 83.23 cm and 96.85 cm away from tank′s center from 1^st to 8^th day, respectively. The response time shortens gradually but not obviously from 5^th day and is lower than that of the control from 2^nd day. The aggregation time shortens gradually but not obviously from 4^th day and is lower than that of the control during the whole taming process. The residence time gradually increases but not obviously from 5^th day and is longer than that of the control during the whole process. The aggregation rate reaches the maximum from 4^th day and is higher than that of the control during the whole process. Thus, rectangular continuant at 500 Hz has a distinct attractive effect on juvenile seabream and conditional reflex can be established after 5 d acoustic taming.