Abstract: The complete sequences of mitochondrial Cyt b gene in Pagrus pagrus, Sparus macrocephalus, Acanthopagrus latus and Parargyrops edita in Zhoushan sea area were amplified, cloned and sequenced. The 1 141 bp Cyt b gene sequences of these four species were obtained. Compared with the same DNA fragment recorded in the GeneBank, the sequences of 1 141 bp DNA had 292 nucleotide variation sites, among which 186 parsimony informative sites were observed.Transitions were discovered to be more than transversions, and most of the base substitutions occurred at the third locus of the codon. The genetic distance among 4 Sparidae species ranged from 0.096 7 to 0.227 5. The shortest distance occurred between P.pagrus and P.edita, being 0.096 7, while the longest one between S.macrocephalus and A.latus, being 0.227 5. Moreover, the transition-transversion ratio of the sequence differentiation of 4 species ranged from 2.293 0 to 7.358 7. The NJ phylogenetic tree revealed that 25 species were divided into two clades, and obvious genetic divergence existed between fishes of Sparidae and the other fishes of Percoidea.