静磁场辅助冷冻对鳙鱼肉冻结特性和冻藏品质的影响

Effects of static magnetic field-assisted freezing on freezing characteristics and quality changes of Hypophthalmichthys nobilis

  • 摘要: 传统冻结设备冷冻效率低,且生成的不规则冰晶会加剧水产品的品质劣变,因此亟需探索新型辅助冷冻技术。为探究静磁场辅助冷冻对鳙 (Hypophthalmichthys nobilis) 鱼肉品质的影响及其作用机制,本研究采用不同强度 (0、6、10 mT) 静磁场对鳙鱼肉进行辅助冷冻,通过测定冻结曲线探究其冻结特性,并评估了冻藏鱼肉的解冻损失率、肌肉微观结构、水分分布等指标;进一步检测鱼肉肌原纤维蛋白 (MP) 氧化标志物 (羰基、巯基、二硫键、二聚酪氨酸、希夫碱) 含量以及内源荧光性。结果表明,静磁场显著缩短鳙鱼肉冷冻过程中的相变时间 (p<0.05),形成更细小、均匀的冰晶;在冻藏期结束时,6 mT静磁场处理组将鱼肉的解冻损失率降低8.44%,不易流动水占比超过90%,并显著降低丙二醛含量和黄度 (p<0.05);该强度有利于MP维持更低的羰基、二硫键、二聚酪氨酸、希夫碱含量和更高的巯基含量,并有效减少MP的结构展开。综上所述,在冷冻过程中,鳙鱼肉的相变时间随静磁场强度的增加而减少,6 mT静磁场强度对鳙鱼肉冻藏品质的改善效果较佳。

     

    Abstract: The low efficiency and the formation of irregularly shaped ice crystals in traditional freezing equipments lead to severe quality degradation in frozen aquatic products. Consequently, it is urgent to improve the exploration and practical application of novel assisted freezing technologies. To investigate the effects and underlying mechanisms of static magnetic field (SMF)-assisted freezing on the quality of Hypophthalmichthys nobilis fillets during frozen storage, we employed SMF of different intensities (0, 6, 10 mT) to assist the freezing process of H. nobilis. We measured the freezing curves to explore the differences in freezing characteristics, and indicators such as thawing loss, muscle microstructure, and moisture distribution were evaluated. Then we detected the oxidative indicators (Carbonyl groups, sulfhydryl groups, disulfide bonds, dityrosine, and Schiff base) and endogenous fluorescence to characterize myofibrillar protein (MP) oxidation. Results indicate that SMF significantly shortened phase transition time during freezing (p<0.05), forming finer and more uniformly distributed ice crystals. At the end of storage, the 6 mT SMF treatment reduced thawing loss of fish by 8.44%, with over 90% non-flowing water content, and significantly decreased malondialdehyde accumulation and yellowness (p<0.05). Besides, this SMF intensity favored maintaining lower content levels of carbonyl groups, disulfide bonds, dityrosine, and Schiff base in MP, while preserving higher sulfhydryl content and effectively reducing MP structural unfolding. In summary, the phase transition time of bighead carp decreases with increasing SMF intensity during freezing, with an intensity of 6 mT demonstrating the better effect on improving frozen storage quality.

     

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