胡晓娟, 杨铿, 文国樑, 苏浩昌, 许云娜, 徐创文, 徐煜, 徐武杰, 曹煜成. 溶藻菌CZBC1在氯化物型盐碱水中对铜绿微囊藻的溶藻效果研究[J]. 南方水产科学, 2024, 20(5): 169-175. DOI: 10.12131/20240142
引用本文: 胡晓娟, 杨铿, 文国樑, 苏浩昌, 许云娜, 徐创文, 徐煜, 徐武杰, 曹煜成. 溶藻菌CZBC1在氯化物型盐碱水中对铜绿微囊藻的溶藻效果研究[J]. 南方水产科学, 2024, 20(5): 169-175. DOI: 10.12131/20240142
HU Xiaojuan, YANG Keng, WEN Guoliang, SU Haochang, XU Yunna, XU Chuangwen, XU Yu, XU Wujie, CAO Yucheng. Algicidal effect of bacteria CZBC1 on Microcystis aeruginosa in chloride type saline-alkali water[J]. South China Fisheries Science, 2024, 20(5): 169-175. DOI: 10.12131/20240142
Citation: HU Xiaojuan, YANG Keng, WEN Guoliang, SU Haochang, XU Yunna, XU Chuangwen, XU Yu, XU Wujie, CAO Yucheng. Algicidal effect of bacteria CZBC1 on Microcystis aeruginosa in chloride type saline-alkali water[J]. South China Fisheries Science, 2024, 20(5): 169-175. DOI: 10.12131/20240142

溶藻菌CZBC1在氯化物型盐碱水中对铜绿微囊藻的溶藻效果研究

Algicidal effect of bacteria CZBC1 on Microcystis aeruginosa in chloride type saline-alkali water

  • 摘要: 为研发氯化物型盐碱水体溶藻菌菌剂,选取溶藻菌菌株CZBC1,探讨了滤液组、菌体组、菌液组在初始铜绿微囊藻 (Microcystis aeruginosa) 密度分别为105和106个·mL−1的氯化物型盐碱水中对铜绿微囊藻的溶藻效果。结果发现,菌株CZBC1同时具有直接和间接2种溶藻方式,菌株CZBC1的滤液组、菌体组、菌液组作用于铜绿微囊藻时能使藻细胞变形、褪色或破裂,在氯化物型盐碱水体中可以发挥良好的溶藻效果。当初始藻密度为105个·mL−1时,第6天菌液组的铜绿微囊藻密度可降至2.30×10个·mL−1,菌体组降至1.27×102个·mL−1,对照组为3.67×105个·mL−1,菌液组和菌体组极显著低于对照组 (p<0.01),2组的最高溶藻率可达99%以上。当初始藻密度为106个·mL−1时,第6天菌液组的铜绿微囊藻密度可降至8.30×105 个·mL−1,对照组为3.17×106个·mL−1,菌液组显著低于对照组 (p<0.05),最高溶藻率为78.8%。从溶藻效果来看,菌液组>菌体组>滤液组。研究表明,溶藻菌CZBC1在氯化物型盐碱水中对铜绿微囊藻具有良好的溶藻效果,可用于氯化物型盐碱池塘中的微囊藻水华防控。

     

    Abstract: In order to develop the algaolytic bacterial agent for chloride type saline-alkali water, we selected the algaolytic bacterial strain Bacillus cereus CZBC1, and investigated the algaolytic effects of the filtrate group (FG), the bacterium group (BG), and the bacterial solution group (BSG) in the chloride type saline-alkali water with the initial density of Microcystis aeruginosa of 105 and 106 cell·mL−1, respectively. The results show that the strain CZBC1 had both direct and indirect ways of dissolving microalgae. When strain CZBC1 in FG, BG and BSG acted on M. aeruginosa, their microalgal cells could deform, fade or rupture, playing a good role in dissolving algae in chloride type saline-alkali water. When the initial microalgal density was 105 cell·mL−1, the microalgal density could be reduced to 2.30×10 cell·mL−1 in BSG and 1.27×102 cell·mL−1 in BG on the 6th day, which were extremely significantly lower than that in the control group (CG) (3.65×105 cell·mL−1, p<0.01), and the highest algicidal rates in both groups could reach over 99%. When the initial microalgal density was 106 cell·mL−1, the microalgal density could be reduced to 8.30×105 cell·mL−1 in BSG on the 6th day, which was significantly lower than that in CG (3.17×106 cell·mL−1, p<0.05), and its highest algicidal rate could reach over 78.8%. The algicidal effect of BSG was better than that of BG and FG. The results indicate that in order to prevent and control microcystis blooms in chloride type saline alkali ponds, it is recommended to prioritize prevention and regularly apply algicidal bacteria CZBC1 to prevent and control the formation of harmful algal blooms.

     

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