陈静妮, 仲颖, 黄春萍, 李硕, 黄锦炉, 王贵平, 赵立宁. 抗副溶血弧菌卵黄抗体制备及其与不同血清型菌株交叉反应研究[J]. 南方水产科学, 2024, 20(5): 176-185. DOI: 10.12131/20240085
引用本文: 陈静妮, 仲颖, 黄春萍, 李硕, 黄锦炉, 王贵平, 赵立宁. 抗副溶血弧菌卵黄抗体制备及其与不同血清型菌株交叉反应研究[J]. 南方水产科学, 2024, 20(5): 176-185. DOI: 10.12131/20240085
CHEN Jingni, ZHONG Ying, HUANG Chunping, LI Shuo, HUANG Jinlu, WANG Guiping, ZHAO Lining. Preparation of anti-Vibrio parahaemolyticus IgY and its cross-reaction with different serotype strains[J]. South China Fisheries Science, 2024, 20(5): 176-185. DOI: 10.12131/20240085
Citation: CHEN Jingni, ZHONG Ying, HUANG Chunping, LI Shuo, HUANG Jinlu, WANG Guiping, ZHAO Lining. Preparation of anti-Vibrio parahaemolyticus IgY and its cross-reaction with different serotype strains[J]. South China Fisheries Science, 2024, 20(5): 176-185. DOI: 10.12131/20240085

抗副溶血弧菌卵黄抗体制备及其与不同血清型菌株交叉反应研究

Preparation of anti-Vibrio parahaemolyticus IgY and its cross-reaction with different serotype strains

  • 摘要: 为探索卵黄抗体对防控凡纳滨对虾 (Litopenaeus vannamei) 养殖中副溶血弧菌 (Vibrio parahaemolyticus) 病的潜在作用,将2种血清型 (O1:KUT和OUT:KUT) 的3株副溶血弧菌制备成灭活疫苗,分别免疫产蛋鸡制备卵黄抗体。通过ELISA实验和试管凝集实验,评估了卵黄抗体的效价消长规律、抑菌效果和交叉反应情况。结果显示:3株副溶血弧菌均能诱导高特异性水平的卵黄抗体,抗体凝集效价和ELISA效价的消长趋势相近,均在4免后10~60 d达到卵黄抗体的效价平台期,凝集效价在1∶8以上,ELISA效价在1∶6 400以上;卵黄抗体与血清型相同的其他副溶血弧菌发生强烈的交叉ELISA和交叉凝集反应,但与血清型不同的副溶血弧菌有较低的交叉ELISA反应,无交叉凝集反应。研究表明副溶血弧菌能刺激蛋鸡产生高特异性水平的卵黄抗体,对不同血清型菌株不具有交叉保护效果,可为抗副溶血弧菌卵黄抗体产品的开发与应用提供参考。

     

    Abstract: In order to investigate the potential role of immunoglobulin of egg yolk (IgY) in the prevention and control of Vibrio parahaemolyticus in Litopenaeus vannamei culture, we prepared three V. parahaemolyticus strains from serotypes (O1: KUT and OUT: KUT) as inactivated vaccines and immunized against laying hens to prepare IgY, respectively. Then we evaluated the titer, inhibitory effect and cross reaction of anti-V. parahaemolyticus IgY by ELISA and tube agglutination tests. The results show that all the three strains induced high specific level of IgY. The trends of antibody agglutination titer and ELISA titer were similar, both reaching the plateau of antibody titer from 10 to 60 d after the fourth immunization, with agglutination titer above 1∶8 and ELISA titer above 1∶6 400. The prepared IgY showed strong cross-ELISA and cross-agglutination reaction with other V. parahaemolyticus for the same serotype, but had lower cross-ELISA reaction and no cross-agglutination reaction with other serotype strains. This study clarifies that V. parahaemolyticus can stimulate the production of high specific level of IgY in laying hens, but does not have cross-protective potency against different serotypes of strains, which provides references for the development and application of anti-V. parahaemolyticus IgY products.

     

/

返回文章
返回