Abstract: To study the disinfection validity of compound peroxymonosulfate powder in aquaculture, we evaluated its killing effect on grass carp (Ctenopharyngodon idella) reovirus (GCRV), channel catfish (Ictalurus punctatus) virus (CCV), Aeromonas hydrophila and Streptococcus agalactiae, and studied the germicidal effects of three factors (Organic matter, temperature and pH). Moreover, we conducted a killing assay of the target bacteria in pond aquaculture water and a pharmacodynamic test of crucian carp (Carassius auratus) with A. hydrophila disease. The results show that the compound peroxymonosulfate powder had a significant killing effect on the four pathogens. Reducing organic matter concentration in bacterial suspension, appropriately increasing temperature or decreasing pH of disinfectant could promote the germicidal effect. In addition, through the killing assay of target bacteria and pharmacodynamic test of crucian carp, we further confirmed the disinfectant validity on target bacteria and significant therapeutic effect of crucian carp infected with A. hydrophila. Thus, the study indicates that compound peroxymonosulfate powder can be used as a potential disinfectant in aquaculture.