刘佳兴, 郭华阳, 朱克诚, 刘宝锁, 张楠, 冼霖, 张殿昌. 低鱼粉饮食中添加半胱氨酸对卵形鲳鲹幼鱼脂质和蛋白质代谢的影响[J]. 南方水产科学, 2023, 19(4): 116-125. DOI: 10.12131/20230030
引用本文: 刘佳兴, 郭华阳, 朱克诚, 刘宝锁, 张楠, 冼霖, 张殿昌. 低鱼粉饮食中添加半胱氨酸对卵形鲳鲹幼鱼脂质和蛋白质代谢的影响[J]. 南方水产科学, 2023, 19(4): 116-125. DOI: 10.12131/20230030
LIU Jiaxing, GUO Huayang, ZHU Kecheng, LIU Baosuo, ZHANG Nan, XIAN Lin, ZHANG Dianchang. Effects of cysteine addition to low-fishmeal diets on metabolism of lipid and protein in juvenile Trachinotus ovatus[J]. South China Fisheries Science, 2023, 19(4): 116-125. DOI: 10.12131/20230030
Citation: LIU Jiaxing, GUO Huayang, ZHU Kecheng, LIU Baosuo, ZHANG Nan, XIAN Lin, ZHANG Dianchang. Effects of cysteine addition to low-fishmeal diets on metabolism of lipid and protein in juvenile Trachinotus ovatus[J]. South China Fisheries Science, 2023, 19(4): 116-125. DOI: 10.12131/20230030

低鱼粉饮食中添加半胱氨酸对卵形鲳鲹幼鱼脂质和蛋白质代谢的影响

Effects of cysteine addition to low-fishmeal diets on metabolism of lipid and protein in juvenile Trachinotus ovatus

  • 摘要: 为研究半胱氨酸对低鱼粉引起的卵形鲳鲹 (Trachinotus ovatus) 幼鱼 (10.05 ± 0.05) g 代谢紊乱的影响,采用动植物蛋白 (鸡粉、大豆浓缩蛋白、发酵豆粕等) 部分替代鱼粉制作卵形鲳鲹基础饮食,分别添加0 (C0组,对照组)、0.30% (C1组)、0.60% (C2组)、0.90% (C3组) 和1.20% (C4组) 半胱氨酸制成5种等氮等脂饲料。56 d的饲养实验结果显示:1) 半胱氨酸通过激活S6K/PI3K/TOR/4E-BP1通路,提高卵形鲳鲹蛋白质合成代谢能力。补充0.6%~0.9%半胱氨酸通过上调肌肉核糖体蛋白S6激酶 (S6K)、雷帕霉素靶蛋白 (TOR)、磷脂酰肌醇-3-激酶 (PI3K)和4E结合蛋白1 (4E-BP1) 基因的mRNA水平,增加肌肉和血清总蛋白 (Total protein, TP) 含量与肌肉粗蛋白含量,降低血氨 (Serum ammonia, SA) 、肌肉和肝脏尿素氮 (Urea nitrogen, UN) 含量,促进肌肉蛋白质沉积。2) 补充0.6%~0.9%半胱氨酸通过下调肌肉中过氧化物酶体增殖物激活受体γ (PPARγ) 基因的mRNA水平,降低乙酰辅酶A羧化酶 (ACC)、脂肪酸合成酶 (FAS) 基因的mRNA水平与酶活水平,抑制脂肪合成代谢;同时,上调肌肉中过氧化物酶体增殖物激活受体α (PPARα) 基因的表达水平,使激素敏感性脂肪酶 (HSL) 和肉毒碱棕榈酰转移酶1 (CPT1) 基因高表达并伴随酶活提高,进而促进肌肉中脂肪酸β氧化反应,减少蛋白质因分解供能所带来的消耗,促进肌肉中蛋白质沉积。

     

    Abstract: To investigate the effect of cysteine on the metabolic disorders of juvenile Trachinotus ovatus (10.05±0.05) g caused by low fishmeal, we prepared a basal pomfret diet by using plant and animal proteins (Chicken meal, soybean protein concentrate, fermented soybean meal, etc.) as partial substitutes for fishmeal, and then added 0 (Group C0, control group), 0.30% (Group C1), 0.60% (Group C2), 0.90% (Group C3) and 1.20% (Group C4) cysteine to make five isonitrogenous and isoenergetic diets. The results of a 56-day feeding trial show that: 1) Cysteine enhanced the protein synthesis and metabolism ability of T. ovatus by activating the S6K/PI3K/TOR/4E-BP1 pathway. Supplementation with 0.6%−0.9% cysteine up-regulated the mRNA levels of ribosomal protein S6 kinase (S6K), target of rapamycin (TOR), phosphoinositide 3-kinase (PI3K) and eukaryotic initiation factor 4E-binding protein 1 (4E-BP1) in muscle, increased total protein (TP) in muscle and serum as well as muscle crude protein content, reduced serum ammonia (SA) and urea nitrogen (UN) in muscle and liver, promoting protein deposition in muscle. 2) Supplementation with 0.6%−0.9% cysteine inhibited lipid anabolism by down-regulating the expression level of peroxisome proliferator-activated receptors gamma (PPARγ) in muscle, decreasing the transcript level and enzyme activity level of Acetyl-CoA carboxylase (ACC) and fatty acid synthetase (FAS), while it up-regulated the expression level of peroxisome proliferator activated receptors-alpha (PPARα) in muscle, resulting in high hormone-sensitive lipase (HSL) and carnitine palmitoyl transferase 1 (CPT1) expression with increasing enzyme activity, which in turn promoted β-oxidation of fatty acids in muscle, reducing protein consumption due to catabolism for energy supply. Thus, protein deposition in muscle is promoted.

     

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