陈秋羽, 郑晓婷, 仲劲松, 陈智兵, 王莹, 梁雪莹, 董宏标, 张家松. 牛蛙胚胎发育的形态特征观察[J]. 南方水产科学, 2023, 19(6): 158-165. DOI: 10.12131/20230015
引用本文: 陈秋羽, 郑晓婷, 仲劲松, 陈智兵, 王莹, 梁雪莹, 董宏标, 张家松. 牛蛙胚胎发育的形态特征观察[J]. 南方水产科学, 2023, 19(6): 158-165. DOI: 10.12131/20230015
CHEN Qiuyu, ZHENG Xiaoting, ZHONG Jinsong, CHEN Zhibing, WANG Ying, LIANG Xueying, DONG Hongbiao, ZHANG Jiasong. Morphological characterization of embryonic development in Rana catesbeiana[J]. South China Fisheries Science, 2023, 19(6): 158-165. DOI: 10.12131/20230015
Citation: CHEN Qiuyu, ZHENG Xiaoting, ZHONG Jinsong, CHEN Zhibing, WANG Ying, LIANG Xueying, DONG Hongbiao, ZHANG Jiasong. Morphological characterization of embryonic development in Rana catesbeiana[J]. South China Fisheries Science, 2023, 19(6): 158-165. DOI: 10.12131/20230015

牛蛙胚胎发育的形态特征观察

Morphological characterization of embryonic development in Rana catesbeiana

  • 摘要: 为探究牛蛙 (Rana catesbeiana) 早期胚胎发育过程并补充其生物学及影像学资料,采用人工养殖的牛蛙作为亲本,自然抱对产卵受精,观察记录其受精卵的胚胎发育过程,并根据胚胎形态的变化和生理特征划分胚胎发育分期表。结果显示,牛蛙受精卵卵径为 (1.41±0.31) mm,吸水后膨胀沉底,且能自转为植物极朝下、动物极朝上的状态。在温度为 (24.5±0.4) ℃的条件下,牛蛙早期胚胎发育过程可划分为7个阶段24个时期,自受精卵发育至鳃盖闭合期共耗时177 h 38 min,总积温为4 249.71 h·℃,整个发育过程呈现前期发育速度较快、后期较慢的趋势。胚胎自肌肉效应期开始,在受到外界刺激时,会做出头尾弯曲的环形收缩反应;而后随着胚胎孵化至鳃盖闭合完成期,胚体即可在水中自由游动。

     

    Abstract: To explore the early embryonic development process of bullfrogs (Rana catesbeiana) and supplement their biological information and imaging data, we obtained the fertilized eggs from artificially bred bullfrogs by natural oviposition to investigate their morphological changes and biological characteristics. The results show that the size of fertilized eggs was (1.41±0.31) mm. At temperature of (24.5±0.4) ℃, the early embryonic development process could be divided into 7 phases and 24 stages. It took 177 h 38 min for the fertilized eggs to reach the operculum closure stage, and the total accumulated temperature was 4 249.71 h·℃. The embryonic development process showed a pattern of a fast speed at the first several stages (From fertilized egg to 64-cell stage) and then a slowdown at the latter stages. When being stimulated by external stimuli, the embryo had an annular contraction response by bending the head and tail. Later, as the embryo hatched until the completion of gill closure, they become tadpoles.

     

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