密度对拟穴青蟹抗氧化、非特异性免疫能力的影响

田瑜; 揭育鵾; 曾祥兵; 乐燕; 刘广鑫; 程长洪; 马红玲; 郭志勋

doi:10.12131/20220312

密度对拟穴青蟹抗氧化、非特异性免疫能力的影响

doi: 10.12131/20220312

田瑜^{1, 2,},
揭育鵾²,
曾祥兵²,
乐燕²,
刘广鑫²,
程长洪²,
马红玲²,
郭志勋^2, ,

1.
水产科学国家级实验教学示范中心 (上海海洋大学)，上海 210306
2.
中国水产科学研究院南海水产研究所/农业农村部南海渔业资源开发利用重点实验室，广东广州 510300

基金项目: 国家虾蟹产业技术体系 (CARS-48)

详细信息

作者简介:
田瑜：田　瑜 (1996—)，男，硕士研究生，研究方向为青蟹病害防治。E-mail: 3053643129@qq.com

通讯作者:
郭志勋 (1970—)，男，研究员，博士，研究方向为水产病害防治。E-mail: guozhixun1@163.com

中图分类号: S 968.25⁺1
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- 被引次数: 0
出版历程
- 收稿日期: 2022-12-07
- 修回日期: 2023-01-09
- 录用日期: 2023-02-08
- 网络出版日期: 2023-02-20

Effect of density on antioxidant and nonspecific immunity of mud crab (Scylla paramamosain)

1.
National Demonstration Center for Experimental Fisheries Science Education, Shanghai Ocean University, Shanghai 201306, China
2.
South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences/Key Laboratory of South China Sea Fishery Resources Exploitation & Utilization, Ministry of Agriculture and Rural Affairs, Guangzhou 510300, China

摘要

摘要: 密度胁迫会对水生动物的免疫和抗氧化功能等产生负面影响。为提高拟穴青蟹 (Scylla paramamosain) 养殖产量潜力，为其养殖业发展提供理论依据，通过将拟穴青蟹置于8 只·m⁻² (低密度组)、16 只·m⁻² (中密度组)、32 只·m⁻² (高密度组) 3种不同密度条件下养殖72 h，研究了密度对拟穴青蟹抗氧化和免疫能力的影响。抗氧化酶活结果表明：高密度组过氧化氢酶 (CAT) 和超氧化物歧化酶 (SOD) 活性均显著高于低密度和中密度组 (P<0.05)，而谷胱甘肽过氧化物酶 (GSH-Px) 活性在各组之间无显著性差异；高密度组丙二醛 (MDA) 含量与低密度和中密度组相比显著升高。免疫酶活结果表明，高密度组酸性磷酸酶 (ACP)、碱性磷酸酶 (AKP) 和溶菌酶 (LZM) 活性均显著低于低密度和中密度组 (P<0.05)，而中密度组ACP、AKP、LZM活性与低密度组无显著性差异。荧光定量结果显示，高密度组热休克蛋白70基因 (HSP70) 表达水平呈先降低后升高趋势，且在养殖第6—第48小时均显著低于低密度和中密度组 (P<0.05)；高密度组Caspase 3基因表达水平在养殖6 h后显著升高，在第24小时达到最高后逐渐下降，但其表达水平始终显著高于低密度组 (P<0.05)。综上，高密度养殖会对拟穴青蟹造成氧化损伤，进而导致其免疫能力下降。16 只·m⁻²的养殖密度对拟穴青蟹抗氧化和免疫能力无显著影响。
- 拟穴青蟹 /
- 养殖密度 /
- 免疫 /
- 抗氧化能力
Abstract: Density stress has a negative impact on the immune and antioxidant functions of aquatic animals. In order to improve the potential production of mud crab (Scylla paramamosain) and provide a theoretical basis for its aquaculture development, we cultured the mud crabs for 72 h at three different densities: 8 ind·m⁻² (Low density group), 16 ind·m⁻² (Medium density group), and 32 ind·m⁻² (High density group) to study the effects of density on the antioxidant and immune capacity of mud crabs. The results of antioxidant enzyme activity show that the activities of catalase (CAT) and superoxide dismutase (SOD) in the high density group were significantly higher than those in the low density and medium density groups (P<0.05), but there was no significant difference in the glutathione peroxidase (GSH-Px) activity among the groups. Additionally, the malondialdehyde (MDA) content in the high density group was significantly higher than that in the low density and medium density groups. The activities of acid phosphatase (ACP), alkaline phosphatase (AKP) and lysozyme (LZM) in the high density group were significantly lower than those in the low density and medium density groups (P<0.05), but there was no significant difference between the medium density group and the low density group. The real-time PCR results indicate that the heat shock protein 70 (HSP70) expression level in the high density group decreased first and then increased, significantly lower than that in low density and medium density groups from 6^th hour to 48^th hour (P<0.05). The expression level of Caspase 3 in the high density group increased significantly after 6 h and decreased gradually after reaching the maximum level at 24^th hour, but was always significantly higher than that in the low density group (P<0.05). In conclusion, high density culture can cause oxidative damage, leading to the decline of immunity of mud crabs. The stock density of 16 ind·m⁻² has no significant effects on the antioxidant and immune abilities of mud crabs.
- Scylla paramamosain /
- Stock density /
- Immunity /
- Antioxidant capacity

HTML全文

图 1 密度对拟穴青蟹鳃组织过氧化氢酶、超氧化物歧化酶、谷胱甘肽过氧化物酶活性和丙二醛含量的影响

注：同一坐标轴上的不同小写字母表示差异显著 (P<0.05)；后图同此。

Figure 1. Effect of density on activities of CAT, SOD, GSH-Px and MDA content in gill tissue of S. paramamosain

Note: Values with different lowercase letters at the same time have significant differences (P<0.05). The same case in the following figures.

下载: 全尺寸图片幻灯片

图 3 密度对拟穴青蟹鳃组织中HSP70和Caspase 3基因表达的影响

Figure 3. Effect of density on expression of HSP70 and Caspase 3 genes in gill tissue of S. paramamosain

下载: 全尺寸图片幻灯片

图 2 密度对拟穴青蟹鳃组织酸性磷酸酶、碱性磷酸酶、溶菌酶活性的影响

Figure 2. Effect of density on activities of ACP, AKP and LZM in gill tissue of S. paramamosain

下载: 全尺寸图片幻灯片

表 1 实时荧光定量PCR引物

Table 1. Real-time fluorescence quantitative PCR primers

引物Primer	引物序列 (5'—3')Primer sequence (5'−3')
RT-caspase 3-F	ACGAAGTGAGGGGATTATGCC
RT-caspase 3-R	CAGCCCATCCAGCGAGC
RT-HSP70-F	AGGACAAGGTGAGCGAAGAGG
RT-HSP70-R	TTGGTGATGATGGGGTTACAGA
RT-18S-F	CCTCGTTCATGGGAGACAAT
RT-18S-R	CTAGTCGACGGATCTCCAGC

下载: 导出CSV

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