晴天和阴天不同光照强度对小头鲔血浆生化指标的影响

王文雯; 周胜杰; 胡静; 付正祎; 肖博; 杜珩轶; 杨蕊; 于刚; 孟祥君; 马振华

doi:10.12131/20220224

晴天和阴天不同光照强度对小头鲔血浆生化指标的影响

doi: 10.12131/20220224

王文雯^{1, 2, 3,},
周胜杰^{2, 3, 4},
胡静^{2, 3, 4},
付正祎^{2, 4},
肖博^{2, 4},
杜珩轶^{2, 4},
杨蕊^{2, 3, 4},
于刚^{2, 3, 4},
孟祥君⁵,
马振华^{1, 2, 3, 4, ,}

1.
大连海洋大学水产与生命学院，辽宁大连 116000
2.
三亚热带水产研究院，海南三亚 572018
3.
海南省深远海渔业资源高效利用与加工重点实验室，海南三亚 572018
4.
中国水产科学研究院南海水产研究所热带水产研究开发中心，海南陵水 572400
5.
三沙蓝海海洋工程有限公司，海南海口 570100

基金项目: 海南省重大科技计划项目 (ZDK2021011)；三亚崖州湾科技城科技专项 (SKJC-2022-PTDX-015)；中国水产科学研究院中央级公益性科研院所基本科研业务费专项资金 (2020TD55, 2020XT0301)；中国-东盟现代海洋渔业技术合作及产业化开发示范；中国水产科学研究院南海水产研究所中央级公益性科研院所基本科研业务费专项资金资助 (2021SD09)；广西创新驱动发展专项资金项目(桂科AA18242031)；国家现代农业产业技术体系 (CARS-47)

详细信息

作者简介:
王文雯 (1999—)，女，硕士研究生，研究方向为鱼类苗种繁育。E-mail: 2473226378@qq.com

通讯作者:
马振华 (1981—)，男，研究员，博士，研究方向为海水鱼类繁育与深远海养殖。E-mail: zhenhua.ma@hotmail.com

中图分类号: S 917.4
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- 被引次数: 0
出版历程
- 收稿日期: 2022-08-22
- 修回日期: 2022-09-23
- 录用日期: 2022-10-18
- 网络出版日期: 2022-11-02

Effects of different light intensity on plasma biochemical indexes of Euthynnus affinis on sunny and cloudy days

WANG Wenwen^{1, 2, 3
,},
ZHOU Shengjie^{2, 3, 4},
HU Jing^{2, 3, 4},
FU Zhengyi^{2, 4},
XIAO Bo^{2, 4},
DU Hengyi^{2, 4},
YANG Rui^{2, 3, 4},
YU Gang^{2, 3, 4},
MENG Xiangjun⁵,
MA Zhenhua^{1, 2, 3, 4
, ,}

1.
School of Fisheries and Life, Dalian Ocean University, Dalian 116000, China
2.
Sanya Tropical Fisheries Research Institute, Sanya 572018, China
3.
Key Laboratory of Efficient Utilization and Processing of Marine Fishery Resources of Hainan Province, Sanya 572018, China
4.
Tropical Fisheries Research and Development Center, South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Lingshui 572400, China
5.
Sansha Lanhai Marine Engineering Co. Ltd., Haikou 570100, China

摘要

摘要: 光照是水产养殖中的一个重要环境因子。探究小头鲔 (Euthynnus affinis) 在晴天和阴天对不同光照强度的生理变化，可为研究其最适养殖环境因子调控提供参考。在晴天和阴天分别设置4个取样时间，研究了在晴天光照强度 [2.7 lx (6:00)、1 116 lx (12:00)、913 lx (18:00)、1 lx (24:00)] 和阴天光照强度 [1.9 lx (6:00)、698 lx (12:00)、192 lx (18:00)、1.4 lx (24:00)] 下，小头鲔血浆中皮质醇、总胆固醇、甘油三酯、丙二醛、髓过氧化物酶活性、溶菌酶活性和免疫球蛋白M浓度的变化。结果显示，皮质醇、丙二醛浓度和溶菌酶活性在晴天的4个时间点均无显著性差异，但均在阴天18:00达到最高值，且阴天18:00的皮质醇、丙二醛浓度显著高于晴天18:00 (P<0.05)，而溶菌酶活性则显著低于晴天18:00 (P<0.05)；总胆固醇浓度分别在晴天12:00和阴天18:00达到最高值，但晴天12:00与阴天12:00无显著性差异，而阴天18:00则显著高于晴天18:00 (P<0.05)；甘油三酯浓度、髓过氧化物酶活性在晴天和阴天6:00均达到最高值，但两者无显著性差异。免疫球蛋白M浓度均在晴天和阴天24:00达到最高值，且晴天24:00显著低于阴天24:00 (P<0.05)。结果表明，小头鲔更适宜在晴天的光照下生存，但会通过积极的生理调节以适应不同天气的光照强度变化，从而减少环境因子对其生理及免疫功能的损伤。
- 小头鲔 /
- 光照强度 /
- 晴天 /
- 阴天 /
- 生化指标
Abstract: Illumination is an important factor in aquaculture. Exploring the changes in the plasma physiology and biochemistry of Euthynnus affinis can provide references for the regulation of its optimal environmental factors. We designed four sampling time to investigate the light intensities on sunny days [2.7 lx (6:00), 1 116 lx (12:00), 913 lx (18:00) and 1 lx (24:00)] and those on cloudy days [1.9 lx (6: 00), 698 lx (12:00), 192 lx (18:00) and 1.4 lx (24:00)], so as to study the changes in the plasma cortisol, total cholesterol, triglyceride, malondialdehyde content, myeloperoxidase activity, lysozyme activity and immunoglobulin M concentration. The results show that there was no significant difference in the cortisol, malondialdehyde concentration and lysozyme activity among the four groups on sunny days, but they all reached the maximum at 18:00 on cloudy days. Besides, the concentraions of cortisol and malondialdehyde were significantly higher than those at 18:00 on sunny days (P<0.05), while the lysozyme activity was significantly lower (P<0.05). The total cholesterol content reached the maximum at 12:00 on sunny day and 18:00 on cloudy day, with no significant difference on sunny day and cloudy day at 12:00. However, it was significantly higher at 18:00 on cloudy days than on sunny days. The triglyceride content and myeloperoxidase activity reached the maximum on both sunny and cloudy days at 6:00, without significant difference. The immunoglobulin M concentration reached the maximum at 24:00 on both sunny and cloudy days, and it was significantly lower on sunny days than on cloudy days at 24:00 (P<0.05). The results show that the E. affinis is more suitable for surviving on sunny days, but it can adapt to the changes of light intensity in different weather by its active physiological adjustment, thereby reducing the damage of environmental factors to its physiological and immune functions.
- Euthynnus affinis /
- Light intensity /
- Sunny day /
- Cloudy day /
- Biochemical index

HTML全文

图 1 晴天和阴天中不同光照强度下小头鲔皮质醇、总胆固醇和甘油三酯日变化规律

注：小写英文字母不同者间差异显著(P<0.05)，后图同此。

Figure 1. Daily change of concentrations of cortisol, total cholesterol and triglyceride of E. affinis with different light intensities on sunny and cloudy days

Note: Different lowercase letters indicate significant differences (P<0.05). The same case in the following figures.

下载: 全尺寸图片幻灯片

图 2 晴天和阴天中不同光照强度下小头鲔抗氧化指标变化

Figure 2. Change of antioxidant index of E. affinis with different light intensities on sunny and cloudy days

下载: 全尺寸图片幻灯片

图 3 晴天和阴天中不同光照强度下小头鲔溶菌酶、免疫球蛋白M日变化

Figure 3. Daily change of lysozyme activity and immunoglobulin M concentration of E. affinis with different light intensities on sunny and cloudy days

下载: 全尺寸图片幻灯片

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