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亚硝酸盐和微塑料胁迫对凡纳滨对虾鳃中免疫、解毒代谢和渗透调节相关指标的影响

邢逸夫 段亚飞 韦政坤 朱轩仪 黄建华 张家松

邢逸夫, 段亚飞, 韦政坤, 朱轩仪, 黄建华, 张家松. 亚硝酸盐和微塑料胁迫对凡纳滨对虾鳃中免疫、解毒代谢和渗透调节相关指标的影响[J]. 南方水产科学. doi: 10.12131/20220176
引用本文: 邢逸夫, 段亚飞, 韦政坤, 朱轩仪, 黄建华, 张家松. 亚硝酸盐和微塑料胁迫对凡纳滨对虾鳃中免疫、解毒代谢和渗透调节相关指标的影响[J]. 南方水产科学. doi: 10.12131/20220176
XING Yifu, DUAN Yafei, WEI Zhengkun, ZHU Xuanyi, HUANG Jianhua, ZHANG Jiasong. Effects of nitrite and microplastic stress on immune, detoxification metabolism and osmoregulation-related indicators in gills of Litopenaeus vannamei[J]. South China Fisheries Science. doi: 10.12131/20220176
Citation: XING Yifu, DUAN Yafei, WEI Zhengkun, ZHU Xuanyi, HUANG Jianhua, ZHANG Jiasong. Effects of nitrite and microplastic stress on immune, detoxification metabolism and osmoregulation-related indicators in gills of Litopenaeus vannamei[J]. South China Fisheries Science. doi: 10.12131/20220176

亚硝酸盐和微塑料胁迫对凡纳滨对虾鳃中免疫、解毒代谢和渗透调节相关指标的影响

doi: 10.12131/20220176
基金项目: 国家自然科学基金项目 (31902343);广州市科学技术协会青年人才托举工程项目 (X20210201039);农业科研杰出人才培养计划 (13210308);中国水产科学研究院南海水产研究所中央级公益性科研院所基本科研业务费专项资金资助 (2021SD19, 2022RC01);中国水产科学研究院基本科研业务费资助项目 (2021XT0604);国家重点研发计划“蓝色粮仓科技创新”专项 (2019YFD0900500)
详细信息
    作者简介:

    邢逸夫 (1998—),男,硕士研究生,研究方向为水产动物环境生理。E-mail: xyf907051631@163.com

    通讯作者:

    张家松 (1971—),男,研究员,博士,从事设施渔业养殖技术研究。E-mail: jiasongzhang@hotmail.com

  • 中图分类号: S 917.4

Effects of nitrite and microplastic stress on immune, detoxification metabolism and osmoregulation-related indicators in gills of Litopenaeus vannamei

  • 摘要: 为研究亚硝酸盐和微塑料单因素及复合胁迫对凡纳滨对虾 (Litopenaeus vannamei) 鳃组织生理功能的影响,将对虾分为对照组、20 mg·L−1亚硝酸盐胁迫组 (NIT)、10 µg·L−1微塑料胁迫组 (MP)、20 mg·L−1亚硝酸盐和10 µg·L−1微塑料复合胁迫组 (NM),于第14天测定对虾鳃中相关指标变化。结果显示,1) 氧化应激指标丙二醛 (MDA)、过氧化氢 (H2O2) 浓度及抗氧化酶超氧化物歧化酶 (SOD)、过氧化氢酶 (CAT)、谷胱甘肽过氧化物酶 (GPx) 活性在胁迫后发生不同程度的改变;2) 解毒代谢指标细胞色素P450基因 (CYP450) 和谷胱甘肽S-转移酶基因 (GST) 相对表达水平胁迫后发生不同程度的紊乱;3) 渗透调节指标如离子转运酶液泡型ATP酶 (VATP)、Na+/H+交换体7 (NHE7)、Na+/K+-ATP酶亚基α (NKA-α)、Na+/K+-ATP酶亚基β (NKA-β)、碳酸酐酶 (CA) 和水通道蛋白TIP4-1 (TIP4)、钙通道蛋白1 (CCP)、氯通道蛋白2 (CLC)、水通道蛋白 (AQP) 基因的相对表达水平胁迫后发生不同程度的紊乱;4) 细胞凋亡因子基因 (CASP-3) 相对表达水平在3个胁迫组均显著降低 (P<0.05)。由此推断,亚硝酸盐和微塑料胁迫会诱导凡纳滨对虾鳃中免疫、解毒代谢和渗透调节相关指标变化,进而影响其正常的生理功能。
  • 图  1  亚硝酸盐和微塑料胁迫对凡纳滨对虾鳃中氧化损伤指标的影响

    注:数据上标不同字母表示组间差异显著 (P<0.05);后图同此。

    Figure  1.  Effects of nitrite and microplastics stress on oxidative damage index in gills of L. vannamei

    Note: Different letters on the bar indicate significant difference among the groups (P<0.05). The same case in the following figures.

    图  2  亚硝酸盐和微塑料胁迫对凡纳滨对虾鳃中抗氧化酶活性的影响

    Figure  2.  Effects of nitrite and microplastics stress on antioxidant enzymes activity in gills of L. vannamei

    图  3  亚硝酸盐和微塑料胁迫对凡纳滨对虾鳃组织免疫基因表达的影响

    Figure  3.  Effects of nitrite and microplastics stress on immune gene expression in gills of L. vannamei

    图  4  亚硝酸盐和微塑料胁迫对凡纳滨对虾鳃组织渗透调节基因表达的影响

    Figure  4.  Effects of nitrite and microplastics stress on osmotic regulation gene expression in gills of L. vannamei

    表  1  本研究所用引物序列

    Table  1.   Primer sequences used in this study

    基因名称
    Gene name
    正向引物 (5'—3')
    Forward primer (5'–3')
    反向引物 (5'—3')
    Reverse primer (5'–3')
    Casp-3ATTATAGAGTGCCTGCGTTGCTACCTGCGATATCATGCCTAGATGCTTGG
    CYP450CGCCGCCAAGGAAGTGAAGGTGGGAGGCGATGGTGGTGTC
    GSTACGAACACTACGAACAGAAGGATGCGCCAGGAAGTCGATGTAGGTTAGC
    VATPGCCAAGTATGAGCACCTAGCAGACTCGTCCACCACCTTACTGAAGAGAG
    TIP4GTCTCTCTGAACCCTTGCCAAACTCGTTGCCTATGATTACCTGCGTCCTC
    CCPCCCTGAGACCTCTGTTTGCTGTGTGGCAGTGTTCTTCGCATGTTCC
    NHECACCGCCATCCTCACCAAGTTCACAGAAGAGCACAGCCACAATACC
    CLCGCCGATAGGAGGTGTGTTGTTCAGCGAAGAAGCCACGCCAGTAGTTC
    NKA-αTGAAATCGTGTTTGCCCGTACCTCACCATCACCAGTTACAGCCACAATG
    NKA-βGAACCCAGCCGACGAAGAATACGCAGCAACAATAGGTGGCAGGTAGC
    AQPGCAGCCATCTTGAAGGGAGTGACACGAGGACGAAGGTGATGAGGAG
    CACCTATTCTGGCTCCCTCACTACCCGTTCATCCTCTGGGCAACACTCG
    β-actinAGCTCATTGTAGAAGGTGTGATGCCTCCTGACCCTGAAGTACCCCATTG
    下载: 导出CSV
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  • 收稿日期:  2022-06-22
  • 修回日期:  2022-08-10
  • 录用日期:  2022-09-11
  • 网络出版日期:  2022-09-16

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