大眼双锯鱼仔、稚、幼鱼的形态发育与生长

李涛; 黄小林; 杨育凯; 林黑着; 胡静; 彭嘉诚; 黄忠; 虞为

doi:10.12131/20220059

大眼双锯鱼仔、稚、幼鱼的形态发育与生长

doi: 10.12131/20220059

李涛^{1, 2,},
黄小林^{1, 2},
杨育凯^{1, 2},
林黑着^{1, 2, ,},
胡静^{1, 3},
彭嘉诚⁴,
黄忠^{1, 2},
虞为^{1, 2}

1.
中国水产科学研究院南海水产研究所/农业农村部南海渔业资源开发利用重点实验室/广东省渔业生态环境重点实验室，广东广州 510300
2.
中国水产科学研究院南海水产研究所深圳试验基地，广东深圳 518121
3.
中国水产科学研究院南海水产研究所热带水产研究开发中心，海南三亚 572018
4.
深圳市鼎雄海洋科技有限公司，广东深圳 518000

基金项目: 深圳市大鹏新区科技创新和产业发展专项资金资助项目 (202001-27)；海南省自然科学基金项目 (320QN360)；中国水产科学研究院院南海水产研究所基本科研业务费专项资金资助 (2020SY01)

详细信息

作者简介:
李涛：李　涛 (1985—)，男，助理研究员，硕士，从事海水鱼类繁殖与育种研究。E-mail: 350073518@qq.com

通讯作者:
林黑着 (1965—)，男，研究员，博士，从事水产动物营养与饲料研究。E-mail: linheizhao@163.com

中图分类号: S 917.4
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- 被引次数: 0
出版历程
- 收稿日期: 2022-03-09
- 修回日期: 2022-06-15
- 录用日期: 2022-07-27
- 网络出版日期: 2022-08-10

Morphological development and growth of larvae, juvenile and young red saddleback clownfish (Amphiprion ephippium)

LI Tao^{1, 2
,},
HUANG Xiaolin^{1, 2},
YANG Yukai^{1, 2},
LIN Heizhao^{1, 2
, ,},
HU Jing^{1, 3},
PENG Jiacheng⁴,
HUANG Zhong^{1, 2},
YU Wei^{1, 2}

1.
South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences/Key Laboratory of South China Sea Fishery Research Exploitation & Utilization, Ministry of Agriculture and Rural Affairs/Guangdong Provincial Key Laboratory of Fishery Ecology and Environment, Guangzhou 510300, China
2.
Shenzhen Base of South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Shenzhen 518121, China
3.
Tropical Fisheries Research and Development Center, South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Sanya 572018, China
4.
Shenzhen DingXiong Marine Technology Co. Ltd., Shenzhen 518000, China

摘要

摘要: 为了解大眼双锯鱼 (Amphiprion ephippium) 早期发育特征，并为其人工繁育提供理论参考依据，对眼双锯鱼仔、稚、幼鱼形态和生长特征进行了连续观察和研究。结果显示，在水温 (27.0±0.5) ℃条件下，大眼双锯鱼受精卵经约184 h孵化，0日龄 (Days after hatching, dah) 仔鱼全长4.472~6.152 mm，身体半透明，鳍膜明显，卵黄囊仅小部分残留，并已开口摄食；7 dah全长5.213~12.985 mm，鳍膜消失，各鳍发育基本完成，头部开始出现纹带，进入稚鱼期，身体中部纹带开始出现，头部纹带形成；23 dah全长大于7.652 mm，身体被鳞，身体中部纹带向腹部延伸，各鳍颜色变为黄色，躯干部以红色素和黄色素为主，进入幼鱼期；28 dah幼鱼中部第二纹带完全形成，其后纹带开始从中部逐步消失；130 dah幼鱼中部纹带完全消失；160 dah幼鱼头部纹带完全消失，此时幼鱼外形、体色与成鱼基本相同。大眼双锯鱼仔、稚、幼鱼生长随日龄的增长呈现先慢后快再慢的生长趋势，大眼双锯鱼胚后发育过程中变化最明显的是头部和身体中部白色纹带的形成与消失。
- 大眼双锯鱼 /
- 仔、稚鱼 /
- 生长发育 /
- 纹带
Abstract: In order to study the early developmental characteristics of red saddleback clownfish (Amphiprion ephippium), and provide a theoretical reference for the breeding of clownfish, we had continuously observed and analyzed the morphological and growth characteristics of larvae, juveniles and young individuals. The body of 0 dah (Days after hatching) individuals (Total length 4.472‒6.152 mm) was translucent with obvious fin membrane, only a small part of yolk sac remaining, and feeding had been started. For 7 dah individuals (Total length 5.213‒12.985 mm), the fin membrane disappeared, and the development of each fin basically completed and the bands began to appear on the head, entering into juvenile stage. Then bands began to appear in the middle of the body and fully formed on the head. For 23 dah individuals (Total length>7.652 mm), the whole body was covered by scales, and the bands in the middle of the body extended to the abdomen, with yellow fins, and the trunk was mainly red pigment and yellow pigment, entering young fish stage. For 28 dah individuals, the middle band fully formed, then the bands gradually disappeared from the middle. For 130 dah individuals, the middle band disappeared completely, while the head band disappeared completely until about 160 dah. At this time, the shape and body color of young fish were the same as that of adult fish. In general, the growth of larval, juvenile and young of A. ephippium showed a trend of slow-rapid-slow trend with the increase of age. The formation and disappearance of white band on the head and middle part of the body were the most obvious features during the early development stage of A.ephippium.
- Red saddleback clownfish Amphiprion ephippium /
- Larvae and juveniles /
- Growth and development /
- Band

HTML全文

图 1 亲鱼产卵、受精卵发育的颜色变化

注：a. 第1天；b. 第2天；c. 第3天；d. 第4天；e. 第5天；f. 第7天y；g. 第8天；h. 第9天。

Figure 1. Color changes of spawning and fertilized egg development of parent fish

Note: a. 1^st day; b. 2^nd day; c. 3^rd day; d. 4^th day; e. 5^th day; f. 7^th day; g. 8^th day; h. 9^th day.

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图 2 大眼双锯鱼胚后发育 (标尺=1 mm)

Figure 2. Postembryonic development of A. ephippium (Bar=1 mm)

下载: 全尺寸图片幻灯片

图 3 大眼双锯鱼仔、幼、稚鱼生长曲线

Figure 3. Growth curve of larval, juvenile and young A. ephippium

下载: 全尺寸图片幻灯片

表 1 大眼双锯鱼不同发育时期全长、体高和眼径的生长比较

Table 1. Total length, body height and eye diameter at different developmental stages of A. ephippium

发育期 Developmental stage	全长 Total length/ mm	体高 Body height / mm	眼径 Eye diameter/ mm
仔鱼期 Larval fish	4.472~6.152	0.812~1.135	0.214~0.353
稚鱼期 Juvenile fish	5.213~12.985	1.104~4.552	0.279~0.758
幼鱼期 Young fish	>7.652	>3.157	>0.613

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参考文献(35)

[1]	叶乐 , 胡静, 王雨, 等. 光周期和光照强度对克氏双锯鱼仔鱼存活、生长和发育的影响[J], 琼州学院学报, 2014, 21(5): 78-86.
[2]	腾力平, 杨担光, 李晓光, 等. 二线小丑鱼的人工繁殖[J]. 水产科学, 2005, 24(2): 26-28.
[3]	鲍鹰, 张鹏, 祝承勇, 等. 红小丑人工繁殖和育苗的初步研究[J]. 海洋科学, 2009, 33(2): 5-10.
[4]	鲍鹰, 张鹏, 祝承勇, 等. 棘颊雀鲷人工繁殖与育苗的初步研究[J]. 海洋科学, 2001, 35(3): 67-71.
[5]	单乐州, 邵鑫斌, 闫茂仓, 等. 透红小丑鱼的繁殖习性、胚胎发育和胚后发育[J]. 水产科技情报, 2015, 42(1): 25-29.
[6]	IGNATIUS B, G RATHORE G, JAGADIS I, et al. Spawning and larval rearing technique for tropical clown fish Amphiprion sebae under captive condition[J]. J. Aquac Trop, 2001, 16(3): 241-249.
[7]	MADHU K, MADHU R. Successful breeding of common clownfish Amphiprion percula under captive conditions in Andaman and Nicobar Island[J]. Fish Chim, 2002, 22(9): 16-17.
[8]	DHANEESH K V, AJITH KUMAR T T, SHUNMUGARAGJ T. Embryonic development of percula clown fish, Amphiprion percula ( Lacepede, 1802)[J]. Middle-East J Sci Res, 2009, 4(2): 84-89.
[9]	HO Y S, CHEN C M, CHEN W Y. Breeding behavior of the pink clownfish (Amphiprion perideraion) and its larval rearing study[J]. J Fish Soc Taiwan, 2005, 32(1): 25-26.
[10]	DHANEESH K V, NANTHINI DEVI K, AJITH KUMAR T T, et al. Breeding embryonic development and salinity tolerance of Skunk clownfish Amphiprion akallopisos[J]. J King Saud Univ Sci, 2012, 24(3): 201-209. doi: 10.1016/j.jksus.2011.03.005
[11]	SUKJAI R, PISUT M, AMARA T, et al. Embryonic development of saddleback anemone fish, Amphiprion polymnus, Linnaeus (1758)[J]. Kaset J Nat Sci, 2005, 39(3): 455-463.
[12]	YASIR I, QIN J. Embryology and early ontogeny of an anemone fish Amphiprion ocellaris[J]. J Mar Bio Assoc UK, 2007, 87(4): 1025-1033. doi: 10.1017/S0025315407054227
[13]	LIEW H J, AMBAK M A, ABOL-MUNAFI A B. Embryonic development of clownfish, Amphiprion ocellaris under laboratory conditions[J]. J Sust Sci Man, 2006, 1(1): 64-73.
[14]	王斌, 王士莉, 杨爱国, 等. 眼斑双锯鱼的人工繁育技术研究[J]. 渔业科学进展, 2010, 31(5): 41-46.
[15]	鞠晨曦, 李云, 刘红, 等. 眼斑双锯鱼胚胎发育的形态学观[J]. 海洋渔业, 2014, 36(1): 8-15.
[16]	何丽斌, 朱志煌, 马敏宁, 等. 毕加索小丑鱼胚胎发育的形态学观察[J]. 应用海洋学报, 2017, 36(4): 545-549.
[17]	殷名称. 鱼类早期生活史研究与其进展[J]. 水产学报, 1991, 15(4): 348-358.
[18]	胡静, 周胜杰, 杨蕊, 等. 眼斑双锯鱼仔稚鱼发育异速生长[J]. 水生生物学报, 2020, 44(4): 844-852.
[19]	刘冬娥, 张雅芝, 方琼珊等. 斜带石斑鱼仔、稚、幼鱼的形态发育研究[J]. 台湾海峡, 2008, 27(2): 180-188.
[20]	陆丽君, 陈超, 马爱军, 等. 云纹石斑鱼 (Epinephelus moara) 早期发育阶段的摄食与生长特性[J]. 海洋与湖沼, 2011(6): 822-829.
[21]	周玲, 翁文明, 李金亮, 等. 鞍带石斑鱼胚胎发育及仔鱼形态发育, 饵料转变的观察研究[J]. 中国农学通报, 2010, 26(1): 293-302.
[22]	张海发, 刘晓春, 刘付永忠, 等. 斜带石斑鱼胚胎及仔稚幼鱼形态发育[J]. 中国水产科学, 2006, 13(5): 689-699.
[23]	陈国华, 张本. 点带石斑鱼仔、稚、幼鱼的形态观察[J]. 海南大学学报 (自然科学版), 2001, 19(2): 151-156.
[24]	王涵生, 方琼珊, 郑乐云. 赤点石斑鱼仔稚幼鱼的形态发育和生长[J]. 上海水产大学学报, 2001, 10(4): 307-312.
[25]	张雅芝, 陈而兴. 春季生殖真鲷仔、稚、幼鱼的摄食与生长[J], 海洋科学, 1998(3): 57-62.
[26]	周宏团, 李加儿, 马荣和, 等. 平鲷胚胎发育及仔、稚、幼鱼的形态观察[J], 水产科技情报, 1990(1): 2-5.
[27]	张孝威, 何桂芬, 沙学绅, 等. 黑鲷卵子及仔、稚、幼鱼的形态观察[J]. 动物学报, 1980(4): 331-336,399-400.
[28]	黄小林, 杨育凯, 李涛, 等. 黄斑篮子鱼仔、稚、幼鱼形态观察与生长研究[J]. 南方水产科学, 2018, 14(5): 88-94.
[29]	PRESTINICOLA L, BOGLIONE C, MAKRIDIS P, et al. Environmental conditioning of skeletal anomalies typology and frequency in gilthead seabream (Sparus aurata L., 1758) juveniles[J]. PLoS ONE, 2013, 8(2): 711-715.
[30]	QIN J G. Larval fish aquaculture [M]. New York: Nova Science Publishers, 2013: 26, 28, 125.
[31]	YUFERA M. Emerging issues in fish larvae research [M]. Switzerland: Springer International Publishing, 2018: 98.
[32]	杨晨. 文昌市云龙湾沿岸造礁石珊瑚生物多样性调查及眼斑双锯鱼的人工繁育[D]. 海口: 海南大学, 2012: 37.
[33]	吕爽, 夏苏东, 高燕, 等. 白条双锯鱼胚胎及仔稚鱼发育形态学观察[J]. 南方农业学报, 2021, 52(12): 3303-3310.
[34]	SALIS P, ROUX N, HUANG D, et al. Thyroid hormones regulate the formation and environmental plasticity of white bars in clownfishes[J]. Pro Nat Aca Sci, 2021, 118(23): e2101634118. doi: 10.1073/pnas.2101634118
[35]	何丽斌, 陈芳, 朱志煌, 等. 基于线粒体16S rRNA、COX1和Cytb基因探讨11种小丑鱼的系统发育关系[J]. 福建农业学报, 2018, 33(3): 230-235.