盐度胁迫对红娇凤凰螺耗氧率、排氨率以及免疫相关酶活性的影响

陈旭; 赵旺; 陈明强; 谭春明; 于刚

doi:10.12131/20210346

盐度胁迫对红娇凤凰螺耗氧率、排氨率以及免疫相关酶活性的影响

doi: 10.12131/20210346

陈旭^{1, 2, 3,},
赵旺^{1, 2, 3},
陈明强^{1, 2, 3},
谭春明^{1, 2},
于刚^{1, 2, 3, ,}

1.
中国水产科学研究院南海水产研究所热带水产研究开发中心/三亚热带水产研究院，海南三亚 572018
2.
农业农村部南海渔业资源开发利用重点实验室，广东广州 510300
3.
海南省深远海渔业资源高效利用与加工重点实验室，海南三亚 527018

基金项目: 国家重点研发计划项目 (2019YFD0900905)；财政部和农业农村部：国家现代农业产业技术体系资助 (CARS-49)；中国水产科学研究院基本科研业务费项目 (2020TD55)；农业农村部财政专项 (NHYYSWZZZYKZX2020)

详细信息

作者简介:
陈旭：陈　旭 (1974—)，男，助理研究员，从事水产养殖和营养与饲料研究。E-mail: chenxu7495@163.com

通讯作者:
于　刚 (1972—)，男，研究员，博士，从事渔业资源开发利用研究。E-mail: gyu0928@163.com

中图分类号: S 966.9
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- 文章访问数: 460
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- 被引次数: 0
出版历程
- 收稿日期: 2021-11-20
- 修回日期: 2022-01-11
- 录用日期: 2022-02-07
- 网络出版日期: 2022-02-21
- 刊出日期: 2022-10-05

Effects of salinity stress on oxygen consumption rate, ammonia excretion rate and immune-related enzyme activities of Strombus luhuanus

CHEN Xu^{1, 2, 3
,},
ZHAO Wang^{1, 2, 3},
CHEN Mingqiang^{1, 2, 3},
TAN Chunming^{1, 2},
YU Gang^{1, 2, 3
, ,}

1.
Tropical Fisheries Research and Development Center, South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences/Sanya Tropical Fisheries Research Institute, Sanya 572018, China
2.
Key Laboratory of South China Sea Fishery Resource Exploitation & Utilization, Ministry of Agriculture and Rural Affairs, Guangzhou 510300, China
3.
Key Laboratory of Efficient Utilization and Processing of Marine Fishery Resources of Hainan Province, Sanya 572018, China

摘要

摘要: 为探明红娇凤凰螺 (Strombus luhuanus) 对盐度变化的适应性，采用静水法研究了不同盐度 (17、22、27、32和37，其中32为对照组) 对红娇凤凰螺耗氧率、排氨率和免疫相关酶活性的影响。结果表明，对照组耗氧率和排氨率显著高于其他实验组 (P<0.05)；各组间总超氧化物歧化酶 (T-SOD) 活性无显著差异 (P>0.05)；与对照组相比，肝胰脏各实验组过氧化氢酶 (CAT) 活性均有不同程度地升高，而肌肉各实验组CAT活性与对照组差异均不显著 (P>0.05)；对照组、盐度27和37组肝胰脏CAT活性均显著高于盐度17和22组 (P<0.05)，而肌肉各实验组 CAT活性均显著高于对照组 (P<0.05)；肝胰脏和肌肉各实验组溶菌酶 (LZM) 活性均显著低于对照组 (P<0.05)。研究表明，盐度胁迫对红娇凤凰螺耗氧率、排氨率以及免疫相关酶活性产生显著影响。
- 红娇凤凰螺 /
- 盐度胁迫 /
- 耗氧率 /
- 排氨率 /
- 免疫相关酶
Abstract: To investigate the adaptability of salinity stress of Strombus luhuanus, we studied the effects of different salinities [17, 22, 27, 32 (control group) and 37] on the oxygen consumption rate, ammonia excretion rate and immune-related enzyme activities of S. luhuanus by hydrostatic method. The results indicate that the oxygen consumption rate and ammonia excretion rate in the control group were significantly higher than those in the other groups (P<0.05). The activity of total superoxide dismutase showed no difference (P>0.05). Compared with the control group, the catalase activity in hepatopancreas groups increased to varying degrees, while the catalase activity in muscle groups was not significantly different (P>0.05). The peroxidase activities of hepatopancreas in the control group, salinity 27 and 37 groups were significantly higher than those in salinity 17 and 22 groups (P<0.05). The peroxidase activities of muscle in the experimental groups were significantly higher than that in the control group (P<0.05). The lysozyme activities of hepatopancreas and muscle in the control group were significantly higher than those in the other experimental groups (P<0.05). In summary, the oxygen consumption rate, ammonia excretion rate and activities of immune-related enzyme have significant effects on S. luhuanus under salinity stress.
- Strombus luhuanus /
- Salinity stress /
- Oxygen consumption rate /
- Ammonia excretion rate /
- Immune-related enzyme

HTML全文

图 1 盐度胁迫对红娇凤凰螺耗氧率、排氨率的影响

注：不同字母表示各组间存在显著差异 (P<0.05)，下同。

Figure 1. Effect of salinity stress on oxygen consumption rate and ammonia excretion rate of S. luhuanus

Note: Different letters indicate significant difference among groups (P<0.05). The same below.

下载: 全尺寸图片幻灯片

图 2 盐度胁迫对红娇凤凰螺肝胰脏免疫酶活性的影响

Figure 2. Effect of salinity stress on immunoenzyme activities of hepatopancreas of S. luhuanus

下载: 全尺寸图片幻灯片

图 3 盐度胁迫对红娇凤凰螺肌肉免疫酶活性的影响

Figure 3. Effect of salinity stress on muscle immunoenzyme activities of S. luhuanus

下载: 全尺寸图片幻灯片

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