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S-亚硝基海藻酸钠抗菌水凝胶的制备与表征

阳星 刘桥 余达威 涂晓亮 田金环 李立华

阳星, 刘桥, 余达威, 涂晓亮, 田金环, 李立华. S-亚硝基海藻酸钠抗菌水凝胶的制备与表征[J]. 南方水产科学, 2022, 18(2): 74-82. doi: 10.12131/20210325
引用本文: 阳星, 刘桥, 余达威, 涂晓亮, 田金环, 李立华. S-亚硝基海藻酸钠抗菌水凝胶的制备与表征[J]. 南方水产科学, 2022, 18(2): 74-82. doi: 10.12131/20210325
YANG Xing, LIU Qiao, YU Dawei, TU Xiaoliang, TIAN Jinhuan, LI Lihua. Preparation and characterization of S-nitrosated sodium alginate antibacterial hydrogel[J]. South China Fisheries Science, 2022, 18(2): 74-82. doi: 10.12131/20210325
Citation: YANG Xing, LIU Qiao, YU Dawei, TU Xiaoliang, TIAN Jinhuan, LI Lihua. Preparation and characterization of S-nitrosated sodium alginate antibacterial hydrogel[J]. South China Fisheries Science, 2022, 18(2): 74-82. doi: 10.12131/20210325

S-亚硝基海藻酸钠抗菌水凝胶的制备与表征

doi: 10.12131/20210325
基金项目: 国家重点研发计划项目 (2019YFD0901905)
详细信息
    作者简介:

    阳星:阳 星 (1998—),女,硕士研究生,研究方向为天然生物材料。E-mail: 862642093@qq.com

    通讯作者:

    李立华 (1977—),女,教授,博士,从事天然生物材料的研究与开发。E-mail: tilihuali@jnu.edu.cn

  • 中图分类号: S 931.9

Preparation and characterization of S-nitrosated sodium alginate antibacterial hydrogel

  • 摘要: 一氧化氮 (NO) 是一种具有高细菌抑制率的气体信号分子,被广泛用于生物医学治疗当中,但是目前还存在如NO供体材料生物相容性差、NO负载量低以及控释缓释效果不佳等问题。以我国盛产的海藻多糖海藻酸钠 (SA) 为原料,制备了巯基化海藻酸钠 (SA-SH) 和NO供体S-亚硝基海藻酸钠 (SA-SNO),并以不同比例复合SA-SH与SA-SNO制备了原位成型水凝胶。结果表明随着SA-SNO含量的增加,NO负载量显著增加;释放曲线表明该凝胶具有较长的释放周期,5 d后NO释放量高达30.12~44.32 μmol·g−1,总释放量为152~264 µmol·mg−1。抗菌实验表明水凝胶对金黄色葡萄球菌 (Staphylococcus aureus)、大肠杆菌 (Escherichia coli) 具有显著抑制作用。综上所述,研究制备的水凝胶具有作为伤口抗菌敷料的应用潜力。
  • 图  1  疏基化海藻酸钠/S-亚硝基海藻酸钠复合水凝胶形成的示意图

    Figure  1.  Schematic diagram of SA-SH/SA-SNO composite hydrogel formation

    图  2  疏基化海藻酸钠合成线路图

    Figure  2.  SA-SH synthetic circuit diagram

    图  3  S-亚硝基海藻酸钠的合成路线图 (a) 和巯基化海藻酸钠与亚硝酸钠反应实图 (b)

    Figure  3.  SA-SNO synthetic circuit diagram (a) and actual picture of reaction of thiolated sodium alginate and sodium nitrite (b)

    图  4  巯基化海藻酸钠与S-亚硝基海藻酸钠紫外扫描图

    Figure  4.  UV-Vis diagrams of thiolated sodium alginate and S-nitrosated sodium alginate

    图  5  巯基化海藻酸钠与S-亚硝基海藻酸钠红外谱图

    Figure  5.  Infrared spectra of thiolated sodium alginate and S-nitrosated sodium alginate

    图  6  巯基化海藻酸钠与S-亚硝基海藻酸钠XPS光谱图

    Figure  6.  XPS spectra of thiolated sodium alginate and S-nitrosoalginate sodium

    图  7  水凝胶120 h内一氧化氮实时释放曲线

    Figure  7.  Real-time release curve of NO from hydrogels in 120 h

    图  8  水凝胶的抑菌圈测试

    Figure  8.  Inhibition zone test of hydrogels

    图  9  与水凝胶共培养1、6、12、24 h的细菌形貌

    Figure  9.  Morphology of bacteria co-cultured with hydrogel for 1, 6, 12, 24 h

    表  1  水凝胶中一氧化氮的总负载量

    Table  1.   Total loading of NO in hydrogel

    复合水凝胶
    Composite hydrogel
    巯基化海藻酸钠
    SA-SH/g
    S-亚硝基海藻
    酸钠 SA-SNO/g
    NO总负载
    NO Total load/(µmol·g−1)
    β-GP-10.1200
    β-GP-20.110.01152.19±0.13
    β-GP-30.100.02173.10±0.85
    β-GP-40.080.04205.43±1.21
    β-GP-50.060.06264.19±2.85
    下载: 导出CSV
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  • 收稿日期:  2021-11-02
  • 修回日期:  2022-01-20
  • 录用日期:  2022-01-21
  • 网络出版日期:  2022-02-07
  • 刊出日期:  2022-04-01

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