S-亚硝基海藻酸钠抗菌水凝胶的制备与表征

阳星; 刘桥; 余达威; 涂晓亮; 田金环; 李立华

doi:10.12131/20210325

S-亚硝基海藻酸钠抗菌水凝胶的制备与表征

doi: 10.12131/20210325

1.
暨南大学材料科学与工程系/人工器官及材料教育部工程研究中心，广东广州 511486
2.
江南大学食品学院/江南大学食品科学与技术国家重点实验室，江苏无锡 214122

基金项目: 国家重点研发计划项目 (2019YFD0901905)

详细信息

作者简介:
阳星：阳　星 (1998—)，女，硕士研究生，研究方向为天然生物材料。E-mail: 862642093@qq.com

通讯作者:
李立华 (1977—)，女，教授，博士，从事天然生物材料的研究与开发。E-mail: tilihuali@jnu.edu.cn

中图分类号: S 931.9
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- 文章访问数: 63
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- 被引次数: 0
出版历程
- 收稿日期: 2021-11-02
- 修回日期: 2022-01-20
- 录用日期: 2022-01-21
- 网络出版日期: 2022-02-07
- 刊出日期: 2022-04-01

Preparation and characterization of S-nitrosated sodium alginate antibacterial hydrogel

1.
Department of Materials Science and Engineering, Jinan University/Engineering Research Center of Artificial Organs and Materials, Guangzhou 511486, China
2.
School of Food Science and Technology, Jiangnan University/State Key Laboratory of Food Science and Technology, Wuxi 214122, China

摘要

摘要: 一氧化氮 (NO) 是一种具有高细菌抑制率的气体信号分子，被广泛用于生物医学治疗当中，但是目前还存在如NO供体材料生物相容性差、NO负载量低以及控释缓释效果不佳等问题。以我国盛产的海藻多糖海藻酸钠 (SA) 为原料，制备了巯基化海藻酸钠 (SA-SH) 和NO供体S-亚硝基海藻酸钠 (SA-SNO)，并以不同比例复合SA-SH与SA-SNO制备了原位成型水凝胶。结果表明随着SA-SNO含量的增加，NO负载量显著增加；释放曲线表明该凝胶具有较长的释放周期，5 d后NO释放量高达30.12~44.32 μmol·g⁻¹，总释放量为152~264 µmol·mg⁻¹。抗菌实验表明水凝胶对金黄色葡萄球菌 (Staphylococcus aureus)、大肠杆菌 (Escherichia coli) 具有显著抑制作用。综上所述，研究制备的水凝胶具有作为伤口抗菌敷料的应用潜力。
- 巯基化海藻酸钠 /
- S-亚硝基海藻酸钠 /
- 一氧化氮 /
- 水凝胶 /
- 抗菌
Abstract: Nitric oxide (NO) is a gas signaling molecule with high bacterial inhibition rate, which has been widely used in biomedical treatment. However, there are still problems such as poor biocompatibility of NO donor materials, low NO load, poor control release and slow-release effect. In this study, we used an aquatic polysaccharide material, sodium alginate (SA), to prepare sulfhydryl sodium alginate (SA-SH) and NO donor S-nitroso sodium alginate (SA-SNO), and prepared in-situ formed hydrogel by using different ratios of SA-SH and SA-SNO. The results show that the NO loading in the hydrogels increased with the increasing content of SA-SNO, and the release curve indicates that the gel possessed long-lasting release effects, wherein the releasing amount of NO arrived to 30.12−44.32 μmol·g⁻¹ even after 5 d and the total release amount was 152−264 μmol·g⁻¹. Additionally, antibacterial experiments show that the hydrogel had a significant inhibitory effect on Staphylococcus aureus and Escherichia coli. In summary, the composite hydrogel has the potential application as an anti-bacterial wound dressing.
- Sulfhydryl sodium alginate /
- S-nitroso sodium alginate /
- Nitric oxide /
- Hydrogel /
- Antibacterial

HTML全文

图 1 疏基化海藻酸钠/S-亚硝基海藻酸钠复合水凝胶形成的示意图

Figure 1. Schematic diagram of SA-SH/SA-SNO composite hydrogel formation

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图 2 疏基化海藻酸钠合成线路图

Figure 2. SA-SH synthetic circuit diagram

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图 3 S-亚硝基海藻酸钠的合成路线图 (a) 和巯基化海藻酸钠与亚硝酸钠反应实图 (b)

Figure 3. SA-SNO synthetic circuit diagram (a) and actual picture of reaction of thiolated sodium alginate and sodium nitrite (b)

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图 4 巯基化海藻酸钠与S-亚硝基海藻酸钠紫外扫描图

Figure 4. UV-Vis diagrams of thiolated sodium alginate and S-nitrosated sodium alginate

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图 5 巯基化海藻酸钠与S-亚硝基海藻酸钠红外谱图

Figure 5. Infrared spectra of thiolated sodium alginate and S-nitrosated sodium alginate

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图 6 巯基化海藻酸钠与S-亚硝基海藻酸钠XPS光谱图

Figure 6. XPS spectra of thiolated sodium alginate and S-nitrosoalginate sodium

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图 7 水凝胶120 h内一氧化氮实时释放曲线

Figure 7. Real-time release curve of NO from hydrogels in 120 h

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图 8 水凝胶的抑菌圈测试

Figure 8. Inhibition zone test of hydrogels

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图 9 与水凝胶共培养1、6、12、24 h的细菌形貌

Figure 9. Morphology of bacteria co-cultured with hydrogel for 1, 6, 12, 24 h

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表 1 水凝胶中一氧化氮的总负载量

Table 1. Total loading of NO in hydrogel

复合水凝胶 Composite hydrogel	巯基化海藻酸钠 SA-SH/g	S-亚硝基海藻酸钠 SA-SNO/g	NO总负载 NO Total load/(µmol·g⁻¹)
β-GP-1	0.12	0	0
β-GP-2	0.11	0.01	152.19±0.13
β-GP-3	0.10	0.02	173.10±0.85
β-GP-4	0.08	0.04	205.43±1.21
β-GP-5	0.06	0.06	264.19±2.85

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