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鳕鱼皮源医用复合止血敷料的制备及性能研究

宋雪 刘晗 李昭璇 李八方 侯虎

宋雪, 刘晗, 李昭璇, 李八方, 侯虎. 鳕鱼皮源医用复合止血敷料的制备及性能研究[J]. 南方水产科学, 2022, 18(2): 66-73. doi: 10.12131/20210323
引用本文: 宋雪, 刘晗, 李昭璇, 李八方, 侯虎. 鳕鱼皮源医用复合止血敷料的制备及性能研究[J]. 南方水产科学, 2022, 18(2): 66-73. doi: 10.12131/20210323
SONG Xue, LIU Han, LI Zhaoxuan, LI Bafang, HOU Hu. Preparation and properties of medical compound hemostasis dressing from Pacific cod skin[J]. South China Fisheries Science, 2022, 18(2): 66-73. doi: 10.12131/20210323
Citation: SONG Xue, LIU Han, LI Zhaoxuan, LI Bafang, HOU Hu. Preparation and properties of medical compound hemostasis dressing from Pacific cod skin[J]. South China Fisheries Science, 2022, 18(2): 66-73. doi: 10.12131/20210323

鳕鱼皮源医用复合止血敷料的制备及性能研究

doi: 10.12131/20210323
基金项目: 国家重点研发计划项目 (2019YFD0901905);山东省泰山学者项目 (tsqn202103033)
详细信息
    作者简介:

    宋雪:宋 雪 (1997—),女,硕士研究生,研究方向为水生生物胶原蛋白。E-mail: 291686910@qq.com

    通讯作者:

    侯 虎 (1984—),男,教授,博士,从事海洋活性物质研究。E-mail: houhu@ouc.edu.cn, houhuouc@163.com

  • 中图分类号: R 318.08

Preparation and properties of medical compound hemostasis dressing from Pacific cod skin

  • 摘要: 以太平洋鳕 (Gadus macrocephalus) 鱼皮明胶为原料,通过复配海藻酸钠得到一种可吸收复合止血敷料,并对其机械性能、止血效果、止血机理和生物相容性进行评价。结果表明,明胶质量浓度为10 mg·mL−1时制备的止血敷料具有较高的机械强度。体内止血评价结果显示,复合止血敷料对大鼠股动脉止血、肝创面止血和断尾止血模型均有良好的效果,止血时间分别为 (64±9)、(108±4) 和 (230±25) s,与市售明胶海绵的止血效果相当。复合止血敷料可缩短活化部分凝血活酶时间 (Activated partial thromboplastin time, APTT) 和凝血酶时间 (Thrombin time, TT),激活内源性凝血系统和共同性凝血途径,以及促进血栓烷素B2 (TXB2)、血小板第四因子 (PF4) 和P-选择素的释放,达到快速止血效果。生物相容性实验结果证实,该复合敷料无急性毒性、无刺激性且溶血率小于5%,达到医用材料的行业标准。该研究结果为开发新型医用敷料并实现鳕鱼皮高值化利用提供了参考和理论依据。
  • 图  1  鳕鱼皮明胶的SDS-PAGE电泳图

    Figure  1.  SDS-PAGE patterns of Pacific cod skin gelatin

    图  2  复合止血敷料的止血性能评价

    Figure  2.  Hemostatic evaluation in vivo with different model

    图  3  不同样品对活化部分凝血酶时间、凝血酶原时间和凝血酶时间血小板活性释放因子血栓烷素B2、血小板第四因子和P-选择素的影响

    Figure  3.  Effect of different samples on APTT, PT, TT and release of platelet active factors (TXB2, PF4 and P-selection)

    图  4  注射24 h、48 h和72 h后的皮内刺激情况

    Figure  4.  Intradermal stimulation at 24th, 48th and 72nd hour after injection

    表  1  不同明胶浓度对复合止血敷料理化性能的影响

    Table  1.   Physical and chemical properties of composite hemostatic sponge with different gelatin concentration

    明胶质量浓度
    Gelatin mass concentration/
    (mg·mL−1)
    抗张强度
    Tensile
    strength/MPa
    断裂伸长率
    Elongation at
    break/%
    吸水倍数
    Water absorption
    ratio
    持水率
    Water retention
    ratio/%
    交联度
    Degree of
    crosslinking/%
    10.010 0±0.003 7a7.54±0.37a25.47±0.14a21.00±0.20a88.90±0.50a
    50.037 2±0.004 2b7.43±1.31a31.82±0.80b28.03±1.03b63.13±0.63b
    100.082 3±0.002 2c6.81±0.21a49.20±2.24c30.49±2.18b56.68±0.33c
    150.085 9±0.003 6c3.49±0.72b38.15±2.24d37.35±2.37c43.36±2.86d
    200.079 5±0.005 6c1.36±0.30c23.89±0.34a37.99±0.30c44.45±0.57d
    注:同列字母不同者表示显著差异 (P<0.05)。 Note: Values with different letters within the same column have significant difference (P<0.05).
    下载: 导出CSV

    表  2  急性全身毒性试验、皮肤刺激试验和溶血试验结果

    Table  2.   Results of acute systemic toxicity assay, dermal irritation test and hemolysis ratio

    实验组
    Experimental group
    阴性对照组
    Negative control group
    阳性对照组
    Positive control group
    急性毒性试验 Acute systemic toxicity assay
     第0小时体质量 Body mass at 0th hour/kg 0.167 7±0.008 2 0.174 0±0.004 6 0.185 3±0.005 0
     第24小时体质量 Body mass at 24th hour/kg 0.172 8±0.007 4 0.177 9±0.009 7 0.186 2±0.004 2
     第48小时体质量 Body mass at 48th hour/kg 0.184 3±0.008 3 0.182 9±0.004 9 0.178 5±0.007 4
     第72小时体质量 Body mass at 72nd hour/kg 0.183 9±0.009 8 0.182 7±0.013 7 0.179 8±0.007 5
    皮肤刺激试验 Dermal irritation test
     第24小时红斑总数 Sum of erythema at 24th hour/个 4 0
     第48小时红斑总数 Sum of erythema at 48th hour/个 0 0
     第72小时红斑总数 Sum of erythema at 72nd hour/个 0 0
     原发性刺激指数 Primary irritation index PII 0.22 0
    溶血试验 Hemolysis test
     溶血率 Hemolysis ratio/% 1.51±0.30 0.00 100.00
    下载: 导出CSV
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出版历程
  • 收稿日期:  2021-11-01
  • 修回日期:  2021-12-13
  • 录用日期:  2022-01-10
  • 网络出版日期:  2022-01-28
  • 刊出日期:  2022-04-01

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