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尖翅燕鱼早期胚胎发育及仔稚鱼形态观察

刘明鉴 郭华阳 高杰 朱克诚 刘宝锁 郭梁 张楠 杨静文 刘波 张殿昌

刘明鉴, 郭华阳, 高杰, 朱克诚, 刘宝锁, 郭梁, 张楠, 杨静文, 刘波, 张殿昌. 尖翅燕鱼早期胚胎发育及仔稚鱼形态观察[J]. 南方水产科学. doi: 10.12131/20210251
引用本文: 刘明鉴, 郭华阳, 高杰, 朱克诚, 刘宝锁, 郭梁, 张楠, 杨静文, 刘波, 张殿昌. 尖翅燕鱼早期胚胎发育及仔稚鱼形态观察[J]. 南方水产科学. doi: 10.12131/20210251
LIU Mingjian, GUO Huayang, GAO Jie, ZHU Kecheng, LIU Baosuo, GUO Liang, ZHANG Nan, YANG Jingwen, LIU Bo, ZHANG Dianchang. Embryonic development and morphological characteristics of larvae and juvenile of Platax teira[J]. South China Fisheries Science. doi: 10.12131/20210251
Citation: LIU Mingjian, GUO Huayang, GAO Jie, ZHU Kecheng, LIU Baosuo, GUO Liang, ZHANG Nan, YANG Jingwen, LIU Bo, ZHANG Dianchang. Embryonic development and morphological characteristics of larvae and juvenile of Platax teira[J]. South China Fisheries Science. doi: 10.12131/20210251

尖翅燕鱼早期胚胎发育及仔稚鱼形态观察

doi: 10.12131/20210251
基金项目: 国家海洋水产种质资源库—南海种质资源分库国家海洋水产种质资源库项目;中国-东盟海洋生物活体种质资源库的构建和开发利用“中国-东盟海上合作基金”专项;广东省省级科技计划项目“南海海洋生物种质资源库建设与共享服务” (2019B030316030)
详细信息
    作者简介:

    刘明鉴 (1996—),男,硕士研究生,研究方向为水产遗传育种。E-mail: 15222387853@139.com

    通讯作者:

    张殿昌 (1977—),男,研究员,博士,从事水产种质资源与遗传育种研究。E-mail: zhangdch@scsfri.ac.cn

  • 中图分类号: S 962

Embryonic development and morphological characteristics of larvae and juvenile of Platax teira

  • 摘要: 为了提高尖翅燕鱼 (Platax teira) 的卵孵化率和苗种成活率,并为人工催产和苗种繁育提供理论依据,该研究观察分析了尖翅燕鱼受精卵的胚胎发育和仔稚发育的时序特征,对其形态学指标进行测量,推算出仔稚鱼阶段生长特性的回归方程。结果显示,尖翅燕鱼受精卵为浮性卵,直径 (1.29±0.09) mm,卵壳较坚韧,呈黄色透明的球状,单个油球,无黏性。在孵化温度 (26±0.3) ℃、盐度34~36和pH 7.8±0.5条件下,整个胚胎发育经历7个阶段共27个时期,耗时27 h 45 min。初孵仔鱼全长 (3.120±0.349) mm,呈透明状,体表散布着大量分枝叶黄素,待经历仔鱼期 (0~17 d) 和稚鱼期 (17~25 d) ,25 d后进入幼鱼期。此时幼鱼鳍棘和鳍条已发育完全,其典型特征为头部、躯干部和尾部各有三条黑色条带,身体呈雪白的银色。统计模型显示,尖翅燕鱼早期阶段生长速度较缓,7日龄后加速生长;在8日龄仔鱼出现残食和攻击行为。
  • 图  1  尖翅燕鱼胚胎发育

    a. 受精卵;b. 2 细胞期;c. 4 细胞期;d. 8 细胞期;e. 16 细胞期;f. 32 细胞期;g. 64 细胞期;h. 多细胞期;i. 桑葚期;j. 高囊胚期;k. 低囊胚期;l. 原肠早期;m. 原肠中期;n. 原肠晚期;o. 胚体形成;p. 胚孔封闭;q. 眼泡出现期;r. 肌节出现期;s. 色素形成;t. 晶体形成;u. 尾芽期;v. 心跳期;w. 肌肉效应期;x. 耳石形成;y. 孵化前期;z. 孵化期;za. 初孵仔鱼。

    Figure  1.  Embryonic development of P. teira

    a. Fertilized egg; b. 2-cell stage; c. 4-cell stage; d. 8-cell stage; e. 16-cell stage; f. 32-cell stage; g. 64-cell stage; h. Multi-cell stage; i. Morula stage; j. High blastula stage; k. Low blastula stage; l. Early gastrula; m. Middle gastrula; n. Late gastrula; o. Embryo body formation; p. Closure of blastopore; q. Eye vesicle stage; r. Muscle burl stage; s. Formation of pigment; t. Formation of eye lens; u. Caudal bud stage; v. Heart beating stage; w. Muscle contraction; x. Formation of otolish; y. Pre-hatching stage; z. hatching stage; za. Newly hatched larvae.

    图  2  尖翅燕鱼仔稚鱼形态发育

    a. 1龄仔鱼;b. 2日龄仔鱼;c. 3日龄仔鱼;d. 4日龄仔鱼;e. 5日龄仔鱼;f. 7日龄仔鱼;g. 9日龄仔鱼;h. 11日龄仔鱼;i. 13日龄仔鱼;j. 15日龄仔鱼;k. 17日龄仔鱼;l. 19日龄稚鱼;m. 21 日龄稚鱼;n. 23 日龄稚鱼;o. 25日龄幼鱼。

    Figure  2.  Morphological development of larvae and juvenile of P. teira

    a. 1 dph larvae; b. 2 dph larvae; c. 3 dph larvae; d. 4 dph larvae; e. 5 dph larvae; f. 7 dph larvae; g. 9 dph larvae; h. 11 dph larvae; i. 13 dph larva; j. 15 dph larva; k. 17 dph larva; l. 19 dph juvenile; m. 21 dph juvenile; n. 23 dph juvenile; o. 25 dph juvenile.

    图  3  尖翅燕鱼早期生长曲线

    a. 为全长与孵化日龄的关系;b. 为体长与孵化日龄的关系; c. 为体高与孵化日龄的关系;d. 为头长与孵化日龄的关系;e. 为眼径与孵化日龄的关系;f. 卵黄囊体积与孵化日龄的关系。

    Figure  3.  Early growth curve of P. teira

    a. Relationship between total length and day age; b. Relationship between body length and day age; c. Relationship between body height and day age; d. Relationship between head length and day age;e. Relationship between eye diameter and day age; f. Relationship between yolk sac volume and day age.

    表  1  尖翅燕鱼胚胎发育过程

    Table  1.   Embryonic development of P. teira

    发育阶段    
    Development    
    stage    
    发育期     
    Developmental     
    period     
    受精后时间
    Time after
    fertilization
    持续时间
    Duration
    积温
    Accumulative
    Temperature/
    (h·℃)
    图1
    Figure1
    合子期 Zygo phase 受精卵 Fertilized egg 30 min 30 min 13 a
    卵裂期 Cleavage stage 2细胞期 2-cell stage 60 min 30 min 13 b
    4细胞期 4-cell stage 1 h 6 min 16 min 7 c
    8细胞期 8-cell stage 1 h 40 min 36 min 15.6 d
    16细胞期 16-cell stage 1 h 50 min 10 min 4.3 e
    32细胞期 32-cell stage 2 h 10 min 20 min 8.6 f
    64细胞期 64-cell stage 2 h 30 min 20 min 8.6 g
    多细胞期 Multicellular stage 2 h 58 min 28 min 12.1 h
    桑葚期 Morula 3 h 23 min 25 min 12.1 i
    囊胚期 Blastula stage 高囊胚期 High blastula 4 h 7 min 44 min 19.1 g
    低囊胚期 Low blastula 4 h 58 min 51 min 22.1 k
    原肠胚期 Gastrula stage 原肠早期 Early-gastrula stage 5 h 41 min 1 h 13 min 31.6 l
    原肠中期 Mid-gastrula stage 6 h 45 min 34 min 14.7 m
    原肠晚期 Late-gastrula stage 8 h 8 min 1 h 23 min 36 n
    神经胚期 Neurula stage 胚体形成期 Embryogenesis period 9 h 49 min 1 h 41 min 43.8 o
    胚孔封闭期 Closure of blastopore stage 11 h 1 h 11 min 30.8 p
    器官形成期 Organogenesis stage 眼泡出现期 Tail vesicle period 11 h 28 min 28 min 12.1 q
    肌节出现期 Appearance of myomere 12 h 4 min 36 min 15.6 r
    色素形成期 Formation of pigment 13 h 25 min 1 h 21 min 35.1 s
    晶体形成期 Crystal stage of eyes 14 h 42 min 1 h 17 min 33.4 t
    尾芽期 Tail-bud period 16 h 55 min 2 h 13 min 57.6 u
    心跳期 Heart beating period 19 h 18 min 2 h 23 min 62 v
    肌肉效应期 Muscular contraction period 20 h 36 min 1 h 18 min 33.8 w
    耳石形成期 Appearance of statolith 22 h 20 min 2 h 14 min 58.1 x
    孵化前期 Pre-hatching stage 25 h 30 min 2 h 40 min 69.3 y
    孵化期 Hatching stage 26 h 55 min 1 h 25 min 36.0 z
    孵化期 Hatching stage 初孵仔鱼 Newly hatched larvae 27 h 20 min 25 min 10.8 za
    注:受精卵孵化温度为26±0.3℃、盐度34~36和pH 7.8±0.5。 Note: The temperature of hatching seawater was (26±0.3)℃; the salinity was 34~36; the pH was 7.8±0.5.
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  • [1] BILECENOGLU M, KAYA M. A new alien fish in the Mediterranean Sea: Platax teira (Forsskal, 1775) (Osteichthyes: Ephippidae)[J]. Aquat Invasions, 2006, 1(2): 80-83. doi: 10.3391/ai.2006.1.2.5
    [2] ZENG C, LIU B S, ZHU K C, et al. Comparison of body height, body length, full length and body weight growth curves of the longfin batfish, Platax teira[J]. Pak J Zool, 2021, 53(2): 1-7.
    [3] CRESPEL A, ZAMBONINO-INFANTE J L, MAZURAIS D, et al. The development of contemporary European sea bass larvae (Dicentrarchus labrax) is not affected by projected ocean acidification scenarios[J]. Mar Biol, 2017, 164(7): 155. doi: 10.1007/s00227-017-3178-x
    [4] LAGUERRE H, RAYMOND G, PLAN P, et al. First description of embryonic and larval development, juvenile growth of the black sea-cucumber Holothuria forskali (Echinodermata: Holothuroidea), a new species for aquaculture in the north-eastern Atlantic[J]. Aquaculture, 2020, 521: 734961. doi: 10.1016/j.aquaculture.2020.734961
    [5] JIN Y, OLSEN R E, OSTENSEN M-A, et al. Transcriptional regulation of lipid metabolism when salmon fry switches from endogenous to exogenous feeding[J]. Aquaculture, 2019, 503: 422-429. doi: 10.1016/j.aquaculture.2018.12.089
    [6] HONRYO T, TSUKADA H, KURATA M, et al. Suitable light intensity during the ontogenetic development of white trevally, Pseudocaranx dentex (Bloch and Schneider, 1801), larvae[J]. Aquacult Res, 2021, 52: 3508-3517. doi: 10.1111/are.15192
    [7] RIBEIRO F F, FORSYTHE S, QIN J G. Dynamics of intracohort cannibalism and size heterogeneity in juvenile barramundi (Lates calcarifer) at different stocking densities and feeding frequencies[J]. Aquaculture, 2015, 444: 55-61. doi: 10.1016/j.aquaculture.2015.03.029
    [8] ZENG C, LIU B S, ZHU K C, et al. Histological study on development of the digestive system of longfin batfish (Platax teira)[J]. Isr J Aquac-Bamidgeh, 2020, 72: 9.
    [9] LIU B, GUO H Y, ZHU K C, et al. Nutritional compositions in different parts of muscle in the longfin batfish, Platax teira (Forsskal, 1775)[J]. J Appl Anim Res, 2019, 47(1): 403-407. doi: 10.1080/09712119.2019.1649680
    [10] 李松, 李宏, 张明建, 等. 清江河多鳞白甲鱼胚胎发育观察[J]. 中国农业文摘-农业工程, 2021, 33(2): 36-40. doi: 10.3969/j.issn.1002-5103.2021.02.011
    [11] 杨建, 耿龙武, 王雨, 等. 拟赤梢鱼的胚胎发育和仔稚鱼生长特性观察拟赤梢鱼的胚胎发育和仔稚鱼生长特性观察[J]. 水生生物学报, 2021, 45(3): 643-651.
    [12] 张梦淇, 陈超, 李炎璐, 等. 盐度对云纹石斑鱼 (Epinehelus moara ♀) ×鞍带石斑鱼 (Epinehelus lanceolatus ♂) 受精卵孵化的影响及杂交仔稚幼鱼形态发育观察[J]. 渔业科学进展, 2016, 37(4): 81-89. doi: 10.11758/yykxjz.20150312002
    [13] 孙丽华, 陈浩如, 王肇鼎. 盐度对军曹鱼胚胎和仔鱼发育的影响[J]. 生态科学, 2006, 25(1): 48-51. doi: 10.3969/j.issn.1008-8873.2006.01.013
    [14] 黄小林, 李涛, 林黑着, 等. 网箱养殖黄斑篮子鱼胚胎发育观察[J]. 南方水产科学, 2018, 14(2): 96-101. doi: 10.3969/j.issn.2095-0780.2018.02.013
    [15] DOS SANTOS J A, SOARES C M, BIALETZKI A. Early ontogeny of yellowtail tetra fish Astyanax lacustris (Characiformes: Characidae)[J]. Aquacult Res, 2020, 51(10): 4030-4042. doi: 10.1111/are.14746
    [16] 徐永江, 张正荣, 柳学周, 等. 黄条鰤早期生长发育特征[J]. 中国水产科学, 2019, 26(1): 172-182.
    [17] 张亮, 闻海波, 郑冰清, 等. 淡水石首鱼人工催产与胚胎发育观察[J]. 中国水产科学, 2021, 28(5): 569-578.
    [18] RIDDLE M R, HU C K. Fish models for investigating nutritional regulation of embryonic development[J]. Dev Biol, 2021, 476: 101-111. doi: 10.1016/j.ydbio.2021.03.012
    [19] 官曙光, 刘洪军, 李祥东, 等. 黑棘鲷胚胎发育过程及特殊结构观察[J]. 海洋科学, 2011, 35(9): 68-72.
    [20] 何永亮, 区又君, 李加儿. 卵形鲳鲹早期发育的研究[J]. 上海海洋大学学报, 2009, 18(4): 428-434.
    [21] 徐嘉波, 施永海, 谢永德, 等. 池塘养殖金钱鱼的胚胎发育及胚后发育观察[J]. 安徽农业大学学报, 2016, 43(5): 716-721.
    [22] YANAGITSURU Y R, MAIN M A, LEWIS L S, et al. Effects of temperature on hatching and growth performance of embryos and yolk-sac larvae of a threatened estuarine fish: longfin smelt (Spirinchus thaleichthys)[J]. Aquaculture, 2021, 537: 736502. doi: 10.1016/j.aquaculture.2021.736502
    [23] HAN M Y, LUO M, YANG R, et al. Impact of temperature on survival and spinal development of golden pompano Trachinotus ovatus (Linnaeus 1758)[J]. Aquac Rep, 2020, 18: 100556. doi: 10.1016/j.aqrep.2020.100556
    [24] 刘鉴毅, 李琪, 孙艳秋, 等. 多纹钱蝶鱼胚胎发育及胚后发育观察[J]. 中国水产科学, 2021, 28(8): 978-987.
    [25] 周玲, 翁文明, 李金亮, 等. 鞍带石斑鱼胚胎发育及仔鱼形态发育、饵料转变的观察研究[J]. 中国农学通报, 2010, 26(1): 293-302.
    [26] 王亚利, 刘玥, 田佳佳, 等. 温度对四川华鳊仔、稚鱼生长发育及存活率的影响[J]. 渔业科学进展, 2021, 42(5): 47-54.
    [27] THOMAS D, REKHA M U, ANGEL J R J, et al. Effects of salinity amendments on the embryonic and larval development of a tropical brackishwater ornamental silver moony fish, Monodactylus argenteus (Linnaeus, 1758)[J]. Aquaculture, 2021, 544: 737073. doi: 10.1016/j.aquaculture.2021.737073
    [28] SANTOS J A, SOARES C M, BIALETZKI A. Effects of pH on the incubation and early development of fish species with different reproductive strategies[J]. Aquat Toxicol, 2020, 219: 105382. doi: 10.1016/j.aquatox.2019.105382
    [29] PEPE-VICTORIANO R, MIRANDA L, ORTEGA A, et al. Descriptive morphology and allometric growth of the larval development of Sarda chiliensis chiliensis (Cuvier, 1832) in a hatchery in northern Chile[J]. Aquac Rep, 2021, 19: 100576. doi: 10.1016/j.aqrep.2020.100576
    [30] YANGTHONG M, RUENSIRIKUL J. Feed intake stimulation of juvenile spotted scat (Scatophagus argus Linnaeus, 1766) using dietary seaweed supplementation (Ulva sp. )[J]. Aquaculture, 2020, 529: 735626. doi: 10.1016/j.aquaculture.2020.735626
    [31] TONG X, YANG X, BAO C, et al. Changes of biochemical compositions during development of eggs and yolk-sac larvae of turbot Scophthalmus maximus[J]. Aquaculture, 2017, 473: 317-326. doi: 10.1016/j.aquaculture.2017.02.024
    [32] PAPADAKIS I E, KENTOURI M, DIVANACH P, et al. Ontogeny of the eye of meagre (Argyrosomus regius) from hatching to juvenile and implications to commercial larval rearing[J]. Aquaculture, 2018, 484: 32-43. doi: 10.1016/j.aquaculture.2017.10.038
    [33] ZHANG J D, AMENYOGBE E, YANG E J, et al. Feeding habits and growth characteristics of cobia (Rachycentron canadum) larval and juvenile stages[J]. Aquaculture, 2021, 539: 736612. doi: 10.1016/j.aquaculture.2021.736612
    [34] 徐永江, 张正荣, 柳学周, 等. 黄条鰤早期生长发育特征[J]. 中国水产科学, 2019, 26(1): 172-182.
    [35] MANDAL B, KAILASAM M, BERA A, et al. Standardization of oocyte size during artificial fertilization and optimization of stocking density during indoor larval and outdoor nursery rearing of captive spotted scat (Scatophagus argus) for a viable juvenile production system[J]. Aquaculture, 2021, 534: 736262. doi: 10.1016/j.aquaculture.2020.736262
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  • 收稿日期:  2021-09-02
  • 修回日期:  2021-10-10
  • 网络出版日期:  2021-11-02

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