Study on extraction, identification and stability of UV resistant MAAs from Porphyra haitanensis in cosmetics system
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摘要: 以坛紫菜 (Porphyra haitanensis)为原料,提取抗紫外辐射物质类菌胞素氨基酸 (Mycosporine-like amino acids, MAAs),通过紫外可见光谱、液质联用对其进行表征,研究了化妆品体系中的各因素 (pH、光照、温度、防腐剂、抗氧化剂、功能性添加剂) 对MAAs化合物稳定性的影响。结果表明,坛紫菜中MAAs类化合物的种类为Shionrine和Porphyra-334;MAAs在pH6的微酸环境中稳定性最高;紫外照射10 h能使溶液的吸光度下降94.4%;48 ℃高温放置56 d后吸光度下降了28.3%;48 ℃下添加小分子肽溶液的吸光度提高了82%,添加透明质酸溶液的吸光度提高了18.6%,能显著增强MAAs化合物的稳定性;其他常用防腐剂、抗氧化剂和功能性添加剂对MAAs的稳定性无明显影响。Abstract: Taking Porphyra haitanensis as raw material, we extracted the mycosporine-like amino acids (MAAs) and charaterized them by UV Vis spectroscopy and Liquid Chromatograph-Mass Spectrometer (LC-MS), so as to study the effects of various factors in the cosmetic system (pH, light, temperature, preservatives, antioxidants and functional additives) on the stability of MAAs. The results show that Shionrine and Porphyra-334 were the main MAAs in P. haitanensis. MAAs had the highest stability in the weak acid environment with pH=6. UV irradiation for 10 h could decrease the absorbance value by 94.4%. After having been placed at 48 ℃ for 56 d, its absorbance value decreased by 28.3%. At 48 ℃, the addition of small peptide and hyaluronic acid increased the absorbance value of the solution by 82% and 18.6%, respectively, which could improve the stability of MAAs compounds significantly. Other commonly used preservatives, antioxidants and functional additives had no significant effects on the stability of the MAAs.
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Key words:
- Porphyra haitanensis /
- MAAs compounds /
- Anti-UV /
- Cosmetics /
- Stability
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表 1 总离子流图的具体峰参数
Table 1. Specific peak parameters of total ion chromatogram
峰
Peak出峰时间
peak time峰类型
Peak type峰高
Peak height修正面积
Corrected area修正面积占总比数
Percentage/%1 4.326 47 rVB 4 503 618 7 485 541 19.529 2 6.059 186 rVV 3 124 289 7 248 372 19.911 3 8.296 360 rBV 2 802 473 7 692 893 20.070 4 9.146 440 rBV2 677 378 7 131 761 18.606 5 9.971 503 rBV3 392 427 3 211 642 8.379 -
[1] SOLOMON S, ALCAMO J, RAVISHANKARA A R. Unfinished business after five decades of ozone-layer science and policy[J]. Nat Commun, 2020, 11(1): 1-4. doi: 10.1038/s41467-019-13993-7 [2] 张贺, 广海军. 臭氧层破坏对环境产生的影响及预防措施[J]. 资源节约与环保, 2020, 17(5): 6-7. doi: 10.3969/j.issn.1673-2251.2020.05.011 [3] 吕佳桐, 林海生, 秦小明, 等. 牡蛎及其酶解产物抗皮肤光老化的初步研究[J]. 南方水产科学, 2021, 17(1): 91-100. doi: 10.12131/20200141 [4] JANSEN M A K, BILGER W, HIDEG E, et al. Interactive effects of UV-B radiation in a complex environment[J]. Plant Physiol Bioch, 2019, 134: 1-8. doi: 10.1016/j.plaphy.2018.10.021 [5] GROSSET C, PÉRON F. Effect of ultraviolet radiation on vertebrate animals: update from ethological and medical perspectives[J]. Photoch Photobio Sci, 2020, 19(6): 752-762. doi: 10.1039/C9PP00488B [6] 张苗苗. 紫菜中类菌孢素氨基酸 (MAAs) 制备工艺的研究[D]. 青岛: 中国海洋大学, 2015: 3-8. [7] 王晨. 新型紫外线吸收剂的合成及应用[D]. 太原: 山西大学, 2020: 1-5. [8] 刘瑾姝, 马晓燕, 邢建伟, 等. 苦丁茶黄酮提取物对真丝织物抗紫外线性能研究[J]. 丝绸, 2020, 57(10): 1-5. doi: 10.3969/j.issn.1001-7003.2020.10.001 [9] ZHOU Y J, QIAN Y, WANG J Y, et al. Bioinspired lignin-polydopamine nanocapsules with strong bioadhesion for long-acting and high-performance natural sunscreens[J]. Biomacromolecules, 2020, 21(8): 3231-3241. doi: 10.1021/acs.biomac.0c00696 [10] LIN T Y, WU P Y, HOU C W, et al. Protective effects of sesamin against UVB-induced skin inflammation and photodamage in vitro and in vivo[J]. Biomolecules, 2019, 479(9): 1-23. [11] KUMAR J P, MANDAL B B. The inhibitory effect of silk sericin against ultraviolet-induced melanogenesis and its potential use in cosmeceutics as an anti-hyperpigmentation compound[J]. Photochem Photobiol Sci, 2019, 18(10): 2497-2508. doi: 10.1039/C9PP00059C [12] ZHENG Y T, PAN C X, ZHANG Z J, et al. Antiaging effect of Curcuma longa L. essential oil on ultraviolet-irradiated skin[J]. Microchem J, 2020, 154(6): 1-6. [13] 张凯, 王聪聪, 李雅潇, 等. 天然产物抗紫外线辐射研究进展[J]. 武警后勤学院学报(医学版), 2018, 27(12): 1043-1048. [14] 罗亚雄. 鱼腥草天然染料的提取、染色及抗紫外性能研究[D]. 武汉: 武汉纺织大学, 2017: 1-7. [15] 焦天慧, 芦宇, 叶琳琳, 等. 超声波辅助提取红树莓籽中原花青素及其抗紫外活性评价[J]. 中国食品学报, 2019, 19(6): 98-105. [16] 曹机良, 冷国强, 王甜梦, 等. 艾绒提取物对蚕丝织物的染色和抗紫外性能研究[J]. 丝绸, 2019, 56(1): 8-12. doi: 10.3969/j.issn.1001-7003.2019.01.002 [17] 漆晴. 茜草天然染料染色特性及抗紫外性能研究[D]. 武汉: 武汉纺织大学, 2018: 54-60. [18] 曾帅. 羊栖菜多酚分离纯化及对小鼠成纤维细胞抗紫外损伤影响[D]. 上海: 上海海洋大学, 2016: 9-11. [19] TRIONE E J, LEACH C M. Light-induced sporulation and sporogenic substances in fungi[J]. Phytopathology, 1969, 59(8): 1077-1083. [20] 张婉. 海萝藻中类菌胞素氨基酸的提取与抗氧化保湿特性研究[D]. 上海: 上海海洋大学, 2016: 5-9. [21] HYLANDER S. Mycosporine-like amino acids (MAAs) in zooplankton[J]. Mar Drugs, 2020, 18(2): 72. doi: 10.3390/md18020072 [22] CHRAPUSTA E, KAMINSKI A, DUCHNIK K, et al. Mycosporine-like amino acids: potential health and beauty ingredients[J]. Mar Drugs, 2017, 15(10): 326. doi: 10.3390/md15100326 [23] HADAGALLI K, KUMAR R, MANDAL S, et al. Structural, compositional and spectral investigation of prawn exoskeleton nanocomposite: UV protection from mycosporine-like amino acids[J]. Mater Chem Phys, 2020, 249: 123002. doi: 10.1016/j.matchemphys.2020.123002 [24] 王杰, 张金荣, 严小军. 类菌胞素氨基酸的结构特征、分布、制备技术及生物活性研究进展[J]. 天然产物研究与开发, 2017, 29(2): 337-348. [25] LALEGERIE F, STIGER-POUVREAU V, CONNAN S. Temporal variation in pigment and mycosporine-like amino acid composition of the red macroalga Palmaria palmata from Brittany (France): hypothesis on the MAA biosynthesis pathway under high irradiance[J]. J Appl Phycol, 2020, 32(4): 2641-2656. doi: 10.1007/s10811-020-02075-7 [26] WERNER N, ORFANOUDAKI M, HARTMANN A, et al. Low temporal dynamics of mycosporine-like amino acids in benthic cyanobacteria from an alpine lake[J]. Freshw Biol, 2021, 66: 169-176. doi: 10.1111/fwb.13627 [27] 张婉, 吴燕燕, 李来好, 等. 海萝中抗紫外辐射物质的提取工艺优化研究[J]. 南方水产科学, 2016, 12(2): 102-109. doi: 10.3969/j.issn.2095-0780.2016.02.015 [28] 应锐, 张朝辉, 段筱杉, 等. 紫菜中类菌孢素氨基酸抗氧化活性研究[J]. 中国海洋药物, 2017, 36(2): 47-54. [29] NISHIDA Y, KUMAGAI Y, MICHIBA S, et al. Efficient extraction and antioxidant capacity of mycosporine-like amino acids from red alga dulse Palmaria palmata in Japan[J]. Mar Drugs, 2020, 18(10): 502. doi: 10.3390/md18100502 [30] 许志恒, 张朝辉, 李八方, 等. 紫菜中类菌胞素氨基酸种类的确定及提取条件优化[J]. 食品工业科技, 2011, 32(2): 187-190. [31] FUENTES-TRISTAN S, PARRA-SALDIVAR R, IQBAL H M N, et al. Bioinspired biomolecules: mycosporine-like amino acids and scytonemin from Lyngbya sp. with UV-protection potentialities[J]. J Photoch Photobio B, 2019, 201: 111684. doi: 10.1016/j.jphotobiol.2019.111684 [32] SUN Y Y, ZHANG N S, ZHOU J, et al. Distribution, contents, and types of mycosporine-like amino acids (MAAs) in marine macroalgae and a database for MAAs based on these characteristics[J]. Mar Drugs, 2020, 18(1): 43. doi: 10.3390/md18010043 -
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