饲料中添加纳米氧化铈对氨氮与嗜水气单胞菌胁迫下中华绒螯蟹的保护效应

孙元琛; 徐冰洁; 曹艺筹; 许颖洁; 秦粉菊; 袁红霞

doi:10.12131/20210209

饲料中添加纳米氧化铈对氨氮与嗜水气单胞菌胁迫下中华绒螯蟹的保护效应

doi: 10.12131/20210209

苏州科技大学化学与生命科学学院，江苏苏州 215009

基金项目: 中国博士后基金项目 (2016M601883)；苏州市科技计划项目 (SNG2017055)

详细信息

作者简介:
孙元琛 (1995—)，男，硕士研究生，研究方向为环境生物技术。E-mail: sunyuancheny@163.com

通讯作者:
秦粉菊 (1976—)，女，教授，博士，从事环境毒理学和纳米营养学研究。E-mail: qinfenju@mail.usts.edu.cn

中图分类号: S 917.4
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- 被引次数: 0
出版历程
- 收稿日期: 2021-07-26
- 修回日期: 2021-10-23
- 录用日期: 2021-11-05
- 网络出版日期: 2021-11-11
- 刊出日期: 2022-06-05

Protective effects of addition of nano cerium oxide in diets on Eriocheir sinensis under ammonia-nitrogen and Aeromonas hydrophila stresses

School of Chemistry and Life Sciences, Suzhou University of Science and Technology, Suzhou 215009, China

摘要

摘要: 为探索中华绒螯蟹 (Eriocheir sinensis) 应对不良水环境胁迫的作用机制，该研究通过在饲料中添加纳米氧化铈 (nCeO₂) ，探究其对氨氮 (NH₃-N) 与嗜水气单胞菌 (Aeromonas hydrophila) 胁迫下中华绒螯蟹免疫力和抗氧化能力的影响。用含不同剂量nCeO₂ (0、0.2、0.4、0.8、1.6、3.2、6.4和12.8 mg·kg⁻¹) 的饲料连续饲喂中华绒螯蟹90 d后进行NH₃-N与嗜水气单胞菌胁迫实验。结果显示：1) NH₃-N单胁迫和NH₃-N与嗜水气单胞菌双胁迫 (下文简称“双胁迫”) 均显著降低了中华绒螯蟹的成活率 (P<0.01)，酸性磷酸酶 (ACP)、碱性磷酸酶 (AKP) 和溶菌酶 (LZM) 活性均显著上升 (P<0.01或P<0.05)，超氧化物歧化酶 (SOD) 和过氧化氢酶 (CAT) 活性均显著下降 (P<0.01)，丙二醛 (MDA)显著上升 (P<0.01或P<0.05)；2) 适量的nCeO₂可显著提升NH₃-N单胁迫和双胁迫下中华绒螯蟹的成活率 (P<0.01或P<0.05)，增强其SOD和CAT活性 (P<0.01)，降低MDA浓度 (P<0.01或P<0.05) ，进一步提升LZM活性 (P<0.01)，但ACP和AKP活性仅在双胁迫下进一步增强 (P<0.01)。综上，适量添加nCeO₂可改善NH₃-N单胁迫和双胁迫所造成的中华绒螯蟹成活率和抗氧化能力下降，提高其免疫酶活性，且添加0.8、1.6 mg·kg⁻¹ nCeO₂分别对单胁迫和双胁迫下的蟹体保护作用最明显。
- 中华绒螯蟹 /
- 纳米氧化铈 /
- 氨氮胁迫 /
- 嗜水气单胞菌 /
- 免疫性能 /
- 抗氧化能力
Abstract: To explore the mechanism of Chinese mitten crabs (Eriocheir sinensis) dealing with adverse water environmental stresses, we studied the effects of nano cerium oxide (nCeO₂) on the immunity and antioxidant capacity of the crabs under ammonia-nitrogen and Aeromonas hydrophila stresses. The crabs were continuously fed with diets supplemented with different doses of nCeO₂ (0, 0.2, 0.4, 0.8, 1.6, 3.2, 6.4 and 12.8 mg·kg⁻¹) for 90 d, then the ammonia-nitrogen and A. hydrophila stress tests were conducted. The results show that: 1) Both the single ammonia-nitrogen stress and dual stresses of ammonia-nitrogen and A. hydrophila (henceforth called "dual stress") decreased the survival rates of the crabs significantly (P<0.01); the activities of acid phosphatase (ACP), alkaline phosphatase (AKP) and lysosome (LZM) increased (P<0.01 or P<0.05); the activities of superoxide dismutase (SOD) and catalase (CAT) decreased significantly (P<0.01), while the concentration of malondialdehyde (MDA) increased (P<0.01 or P<0.05); 2) Suitable nCeO₂ dose could enhance the survival rates of crabs under the single ammonia-nitrogen stress and dual stress (P<0.01 or P<0.05), increase the activities of SOD and CAT significantly (P<0.01), decrease the concentration of MDA (P<0.01 or P<0.05), further increase the activity of LZM (P<0.01), but the activities of ACP and AKP only increased under dual stress (P<0.01). Above all, suitable supplementation of nCeO₂ can ameliorate the decline of survival rates and antioxidant capacity of E. sinensis under the the single ammonia-nitrogen stress and dual stress, and increase the immunity enzyme activities. The suitable nCeO₂ supplementation doses for protecting crabs are 0.8 and 1.6 mg·kg⁻¹ under single and dual stress, respectively.
- Eriocheir sinensis /
- nCeO₂ /
- Ammonia-nitrogen stress /
- Aeromonas hydrophila /
- Immunity property /
- Antioxidant capacity

HTML全文

图 1 不同剂量纳米氧化铈对氨氮与嗜水气单胞菌胁迫下中华绒螯蟹成活率的影响

Figure 1. Effects of different doses of nCeO₂ on survival rates of E. sinensis under ammonia nitrogen and A. hydrophila stresses

下载: 全尺寸图片幻灯片

图 2 不同剂量纳米氧化铈对氨氮与嗜水气单胞菌胁迫下中华绒螯蟹酸性磷酸酶、碱性磷酸酶和溶菌酶活性的影响

Figure 2. Effects of different doses of nCeO₂ on ACP, AKP and LZM activities of E. sinensis under ammonia nitrogen and A. hydrophila stresses

下载: 全尺寸图片幻灯片

图 3 不同剂量纳米氧化铈对不同条件下中华绒螯蟹超氧化物歧化酶、过氧化氢酶活性和丙二醛浓度的影响

Figure 3. Effects of different doses of nCeO₂ on SOD, CAT activities and MDA concentration of E. sinensis under ammonia nitrogen and A. hydrophila stresses

下载: 全尺寸图片幻灯片

表 1 实验设计分组与胁迫处理

Table 1. Experimental group design and stress treatment

纳米氧化铈剂量nCeO₂ dose/ (mg·kg⁻¹)	胁迫处理 Stress treatment	氨氮胁迫NH₃-N stress	嗜水气单胞菌注射A. hydrophila injection
0	空白组	−	−
	单胁迫组	+	−
	双胁迫组	+	+
0.2	空白组	−	−
	单胁迫组	+	−
	双胁迫组	+	+
0.4	空白组	−	−
	单胁迫组	+	−
	双胁迫组	+	+
0.8	空白组	−	−
	单胁迫组	+	−
	双胁迫组	+	+
1.6	空白组	−	−
	单胁迫组	+	−
	双胁迫组	+	+
3.2	空白组	−	−
	单胁迫组	+	−
	双胁迫组	+	+
6.4	空白组	−	−
	单胁迫组	+	−
	双胁迫组	+	+
12.8	空白组	−	−
	单胁迫组	+	−
	双胁迫组	+	+

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