基于宏条形码技术的南海亮眶灯鱼食性初步分析

陈晓雷; 李敏; 陈作志; 张俊; 张帅; 齐占会; 徐姗楠

doi:10.12131/20210199

基于宏条形码技术的南海亮眶灯鱼食性初步分析

doi: 10.12131/20210199

陈晓雷^{1, 2, 3,},
李敏^{1, 3},
陈作志^{1, 3},
张俊^{1, 3},
张帅¹,
齐占会^{1, 3},
徐姗楠^{1, 3, ,}

1.
中国水产科学研究院南海水产研究所/农业农村部外海渔业可持续利用重点实验室/广东省渔业生态环境重点实验室，广东广州 510300
2.
上海海洋大学水产与生命学院，上海 201306
3.
南方海洋科学与工程广东省实验室 (广州)，广东广州 511458

基金项目: 广东省基础与应用基础研究重大项目 (2019B030302004-05)；南方海洋科学与工程广东省实验室 (广州) 人才团队引进重大专项 (GML2019ZD0605)；中国水产科学研究院中央级公益性科研院所基本科研业务费资助 (2020TD16)；中国水产科学研究院南海水产研究所中央级公益性科研院所基本科研业务费专项资金资助 (2021SD04)

详细信息

作者简介:
陈晓雷 (1995—)，男，硕士研究生，研究方向为水生生物学。E-mail: 1458681743@qq.com

通讯作者:
徐姗楠 (1979—)，女，研究员，博士，从事海洋生物学研究。E-mail: xushannan@scsfri.ac.cn

中图分类号: S 932.4
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- HTML全文浏览量: 111
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- 被引次数: 0
出版历程
- 收稿日期: 2021-07-19
- 修回日期: 2021-09-22
- 录用日期: 2021-10-08
- 网络出版日期: 2021-10-19
- 刊出日期: 2022-06-02

Preliminary metabacording dietary analysis of Diaphus splendidus in South China Sea

CHEN Xiaolei^{1, 2, 3
,},
LI Min^{1, 3},
CHEN Zuozhi^{1, 3},
ZHANG Jun^{1, 3},
ZHANG Shuai¹,
QI Zhanhui^{1, 3},
XU Shannan^{1, 3
, ,}

1.
South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences/Key Laboratory for Sustainable Utilization of Open-Sea Fishery, Ministry of Agriculture and Rural Affairs/Guangdong Provincial Key Laboratory of Fishery Ecology and Environment, Guangzhou 510300, China
2.
College of Fisheries and Life Science, Shanghai Ocean University, Shanghai 201306, China
3.
Southern Marine Science and Engineering Guangdong Laboratory (Guangzhou), Guangzhou 511458, China

摘要

摘要: 食性研究是鱼类生物学和生态学研究的重要内容，是进行渔业资源开发和管理的基础。以南海亮眶灯鱼 (Diaphus splendidus) 为研究对象，提取其胃含物DNA，选用线粒体基因细胞色素C氧化酶亚基I (Mitochondrial cytochrome oxidase subunit I, COI) 作为分子标记进行扩增，通过高通量测序鉴定其食物组成。结果显示，亮眶灯鱼胃含物中共鉴定出饵料生物34种，隶属于5门7纲11目18科29属。亮眶灯鱼食物组成包括介形类、桡足类、端足类、鱼类、水母类、磷虾类、翼足类和多毛类。其中介形类、桡足类和端足类是其食物组成中的优势类群，这些优势类群与形态学鉴定方法的结果基本一致，此外还检测出形态学方法未鉴定到的水母这一食物类群。研究表明宏条形码技术适用于南海亮眶灯鱼的食性分析，且比基于形态学的食性分析具有更高的鉴别潜力。
- 灯笼鱼 /
- 亮眶灯鱼 /
- 食性 /
- 宏条形码 /
- 胃含物 /
- 南海
Abstract: Dietary analysis of fish is an important part of fish biology and ecology as well as the basis of fishery resources development. In this study, the gastric DNA of Diaphus splendidus was extracted, and the mitochondrial cytochrome c oxidase subunit I (COI) was amplified as a molecular marker. The food composition was identified by high-throughput sequencing. The results show that a total of 34 species belonged to 5 phyla, 7 classes, 11 orders, 18 families and 29 genera. The diet of D. splendidus mainly included Ostracoda, Copepoda, Amphipoda, Fish, Jellyfish, Euphausiacea, Gastropod and Polychaeta. Ostracoda, Copepoda and Amphipoda were the dominant groups in its food composition, which is basically consistent with the previous research based on traditional morphological identification, except for jellyfish that was identified only by this study. In conclusion, DNA metabarcoding method is suitable for the food analysis of D. splendidus in the South China Sea, and has higher identification potential than the food analysis based on morphology.
- Myctophidae /
- Diaphus splendidus /
- Dietary habits /
- Metabarcoding /
- Stomach content /
- South China Sea

HTML全文

图 1 南海亮眶灯鱼与其胃含物

Figure 1. D. splendidus of South China Sea and its stomach contents

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图 2 亮眶灯鱼胃含物相对丰度组成百分比 (纲水平)

Figure 2. Relative abundance proportion of stomach contents for D. splendidus (class level)

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表 1 亮眶灯鱼生物学信息

Table 1. Sampling information of D. splendidus

样品编号 Sample No.	体长 Body length/mm	体质量 Body mass/g	摄食强度 Feeding intensity
LK-1	58	2.88	1
LK-2	47	1.46	1
LK-3	56	2.89	2
LK-4	53	2.19	2
LK-5	54	2.35	1
LK-6	48	1.62	1
LK-7	47	1.69	1
LK-8	38	0.95	2

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表 2 亮眶灯鱼肌肉和胃含物种类鉴定的PCR引物

Table 2. PCR primers for species identification of muscle and stomach contents of D. splendidus

引物 Primer	序列 (5'—3') Sequence (5'—3')	退火温度 Annealing temperature/℃	片段长度 Fragment length/bp	参考文献 Reference
FishF1	TCAACCAACCACAAAGACATTGGCAC	55	655	[25]
FishR1	TAGACTTCTGGGTGGCCAAAGAATCA	55	655	[25]
mlCOIintF	GGWACWGGWTGAACWGTWTAYCCYCC	62~46	313	[26-27]
jgHCO2198	TAAACTTCAGGGTGACCAAARAAYCA	62~46	313	[26-27]

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表 3 亮眶灯鱼胃含物组成

Table 3. Stomach contents of D. splendidus

纲 Class	饵料生物 Prey species	相对丰度百分比 Percentage of abundance/%	排序 Rank	生物类群 Biome
甲壳纲 Hexanauplia	瘦乳点水蚤 Pleuromamma gracilis	0.10	20	桡足类
	剑乳点水蚤 Pleuromamma xiphias	0.47	8	桡足类
	腹突乳点水蚤 Pleuromamma abdominalis	0.07	24	桡足类
	美丽长腹水蚤 Metridia venusta	0.13	14	桡足类
	普通波水蚤 Undinula vulgaris	0.28	17	桡足类
	波刺水蚤属 Undeuchaeta sp.	0.41	11	桡足类
	茗荷属 Conchoderma sp.	9.83	2	桡足类
介形虫纲 Ostracoda	长拟浮萤 Paraconchoecia oblonga	0.09	22	介形类
	猬刺拟浮萤 Paraconchoecia echinata	0.44	10	介形类
	胖海浮萤 Halocypris inflata	77.70	1	介形类
	吸海萤属 Halocypris sp. USNM IZ 1448950	0.06	27	介形类
	双浮萤属 Discoconchoecia sp. S8-D6	0.67	6	介形类
	短刺直浮萤 Orthoconchoecia secernenda	0.11	19	介形类
	刺额葱萤 Porroecia spinirostris	0.04	30	介形类
软甲纲 Malacostraca	拟遂足磷虾 Thysanopoda aequalis	0.40	12	磷虾类
	武装片戎 Vibilia armata	3.56	3	端足类
	莫氏硬壳寄居蟹 Calcinus morgani	0.07	24	十足类
辐鳍亚纲 Actinopteri	半裸银斧鱼 Argyropelecus hemigymnus	0.03	31	鱼类
	长银斧鱼 Argyropelecus affinis	0.07	24	鱼类
	勃氏圆罩鱼 Cyclothone braueri	0.10	20	鱼类
	大西洋钻光鱼 Gonostoma atlanticum	0.36	13	鱼类
	离光鱼属 Woodsia sp.	0.03	32	鱼类
	黑须黑巨口鱼 Melanostomias tentaculatus	0.06	27	鱼类
	眶灯鱼属 Diaphus sp.	0.12	18	鱼类
	吕氏眶灯鱼 Diaphus luetkeni	0.08	23	鱼类
	眶暗虹灯鱼 Bolinichthys pyrsobolus	0.05	29	鱼类
	后肛鱼 Opisthoproctus soleatus	0.45	9	鱼类
	望远冬肛鱼 Winteria telescopa	0.59	7	鱼类
水螅纲 Hydrozoa	棍手水母属 Rhopalonema sp.	0.23	15	水母类
	Botrynema sp.	2.43	4	水母类
腹足纲 Gastropoda	Clio pyramidata	0.78	5	翼足类
	Diacria major	0.01	34	翼足类
	驼龟螺 Cavolinia gibbosa	0.02	33	翼足类
多毛纲 Polychaeta	叶须虫属 Phyllodoce sp. 11BIOAK-1631	0.16	16	多毛类

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表 4 南海海域不同灯笼鱼胃含物比较

Table 4. Stomach contents of different Myctophidae fishes from South China Sea

生物类群 Biome	亮眶灯鱼 (本研究) D. splendidus (this study)	金鼻眶灯鱼^[14] D. chrysorhynchus	瓦氏眶灯鱼^[15] D. watasei	尾明角灯鱼^[32] Ceratoscopelus warmingii
介形类 Ostrac oda	√	√	√	√
桡足类 Copepoda	√	√	√	√
端足类 Amphipoda	√	√	√	√
鱼类 Fish	√	√	√	√
磷虾类 Euphausiacea	√	√	√	√
腹足类 Gastropod	√	√		√
十足类 Decapoda	√	√
多毛类 Polychaeta	√	√
水母类 Jellyfish	√
萤虾类 Lucifer		√
等足类 Sopoda		√
毛鄂类 Chaetognatha		√
头足类 Cephalopoda		√	√

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