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基于LC-MS技术的海、淡水养殖刀鲚卵巢的代谢组学比较分析

高淑芳 张金鹏 施永海 袁新程 刘其根

高淑芳, 张金鹏, 施永海, 袁新程, 刘其根. 基于LC-MS技术的海、淡水养殖刀鲚卵巢的代谢组学比较分析[J]. 南方水产科学. doi: 10.12131/20210185
引用本文: 高淑芳, 张金鹏, 施永海, 袁新程, 刘其根. 基于LC-MS技术的海、淡水养殖刀鲚卵巢的代谢组学比较分析[J]. 南方水产科学. doi: 10.12131/20210185
GAO Shufang, ZHANG Jinpeng, SHI Yonghai, YUAN Xincheng, LIU Qigen. Metabonomics analysis of ovaries of Coilia nasus in seawater and freshwater based on liquid chromatography-mass spectrometry[J]. South China Fisheries Science. doi: 10.12131/20210185
Citation: GAO Shufang, ZHANG Jinpeng, SHI Yonghai, YUAN Xincheng, LIU Qigen. Metabonomics analysis of ovaries of Coilia nasus in seawater and freshwater based on liquid chromatography-mass spectrometry[J]. South China Fisheries Science. doi: 10.12131/20210185

基于LC-MS技术的海、淡水养殖刀鲚卵巢的代谢组学比较分析

doi: 10.12131/20210185
基金项目: 农业部鱼类营养与环境生态研究中心 (A1-3201-19-300204);上海市科委重点攻关项目(17391900300)
详细信息
    作者简介:

    高淑芳 (1994—),女,硕士研究生,研究方向为刀鲚分子生物学。E-mail: sfgao101@163.com

    通讯作者:

    刘其根 (1965—),男,教授,从事水域生态学研究。E-mail: qgliu@shou.edu.cn

  • 中图分类号: S 965.227

Metabonomics analysis of ovaries of Coilia nasus in seawater and freshwater based on liquid chromatography-mass spectrometry

  • 摘要: 为探究海、淡水养殖环境对刀鲚 (Coilia nasus) 卵巢发育的影响,该研究采用非靶向代谢组学的方法,测定了海、淡水环境下刀鲚卵巢中代谢产物的差异情况,并与基因组百科全书 (KEGG) 数据库进行比对,找出相对应的代谢通路并分析其原因。结果表明,海水组和淡水组样品共鉴定出47种差异代谢物 (P<0.05、FC>1、VIP>1),与海水组相比,淡水组表达差异倍数最明显的为碳环血氧烷A2 (Carbocyclic Thromboxane A2)、半乳糖神经酰胺(Galactosyl ceramide),差异倍数分别为10.40、2.78倍;与海水组相比,淡水组卵巢组织内皮质醇升高了1.61倍;对47种差异代谢物进行KEGG分析发现,变化显著的通路有氨酰-tRNA的生物合成和嘧啶代谢通路 (P<0.05),皮质醇、氨酰-tRNA的生物合成通路、嘧啶代谢通路和鞘磷脂代谢通路可能与刀鲚生殖洄游过程中卵巢发育有关。
  • 图  1  海水组和淡水组的主成分分析 (a) 和正交偏最小二乘判别分析 (b) 得分图

    Figure  1.  PCA (a) and OPLS-DA (b) scores of seawater and freshwater

    图  2  置换检验图

    Figure  2.  Permutation test

    图  3  代谢产物Venn图

    a.阳离子模式;b. 阴离子模式。

    Figure  3.  Venn map of metabolites

    a. Positive ion mode; b. Negative ion mode.

    图  4  差异代谢物筛选火山图

    Figure  4.  Volcanic map of different metabolites

    图  5  代谢物的通路富集分析

    a. 氨酰-tRNA生物合成;b. 嘧啶代谢;c. 鞘磷脂代谢;d. 三羧酸循环。

    Figure  5.  Metabolite pathway enrichment analysis

    a. Aminoacyl-tRNA biosynthesis; b. Pyrimudine metabolism; c. Sphingolipid metabolism; d. Citrate cycle TCA.

    表  1  差异代谢物信息表

    Table  1.   Differential metabolites information sheet

    差异代谢物   
    Differential metabolite   
    分子式
    Molecular formula
    变量投影重要度
    VIP
    FC
    Fold change
    P变化趋势
    Variation trend
    磷脂酰肌醇 PI (20:4(5Z,8Z,11Z,14Z)/0:0) C29H49O12P 1.58 0.93 0.017 下降
    谷氨酰胺色氨酸 Glutaminyltryptophan C16H20N4O4 1.05 0.96 0.018 下降
    L-异亮氨酸 L-Isoleucine C6H13NO2 1.33 0.96 0.005 下降
    木麻黄6-α-D-葡萄糖苷 Casuarine 6-alpha-D-glucoside C14H25NO10 1.13 0.94 0.043 下降
    甘油一脂 MG(10:0/0:0/0:0) C13H26O4 3.59 0.71 0.025 下降
    皮质醇 Cortisol C21H30O5 3.28 1.61 0.008 上升
    碳环血氧烷A2 Carbocyclic thromboxane A2 C22H36O3 2.88 10.40 0.007 上升
    (±)9-十八碳二烯酸 (±)9-HPODE C18H32O4 2.90 0.58 0.020 下降
    N-棕榈酰蛋氨酸 N-palmitoyl methionine C21H41NO3S 1.86 0.75 0.022 下降
    13-羟基十八酸 13-hydroxyoctadecanoic acid C18H36O3 1.95 0.86 0.005 下降
    咖啡酰环戊醇 Caffeoylcycloartenol C39H56O4 2.07 0.81 0.036 下降
    11,13-二十碳二烯酸 15-OxoEDE C20H34O3 2.51 0.70 0.032 下降
    2,3-二氢苯并呋喃 2,3-dihydrobenzofuran C8H8O 1.19 1.08 0.033 上升
    4-甲酰基吲哚 4-formyl indole C9H7NO 1.34 0.83 0.040 下降
    红花素C Safflomin C C30H30O14 2.04 0.85 0.003 下降
    6-[(2-羧基乙酰基)氧]-3,4,5-三羟基氧烷-2-羧酸
    6-[(2-carboxyacetyl)oxy]-3,4,5-trihydroxyoxane-2-carboxylic acid
    C9H12O10 2.07 1.12 0.001 上升
    异戊二烯 Isoputreanine C7H16N2O2 2.33 0.84 0.001 下降
    脯氨酸 L-Proline C5H9NO2 1.80 0.91 0.003 下降
    羟脯氨酸 Hydroxyprolyl-hydroxyproline C10H16N2O5 3.13 0.72 0.007 下降
    5-O-阿魏酰黑精 5-O-Feruloylnigrumin C21H25NO10 1.0 0.95 0.037 下降
    壬二酸 Azelaic acid C9H16O4 1.12 0.96 0.012 下降
    尿苷 Uridine C9H12N2O6 1.35 0.95 0.001 下降
    视黄酯 Retinyl ester C20H30O2 1.24 0.96 0.004 下降
    顺-9,10-环氧硬脂酸 cis-9,10-Epoxystearic acid C18H34O3 1.66 0.92 0.025 下降
    异柠檬酸盐 Isocitrate C6H8O7 1.55 1.12 0.047 上升
    戊二酸 Glutaric acid C5H8O4 2.53 1.38 0.015 上升
    6-脱氧噬菌体胺 (6-脱氧花青) 6-Deoxyfagomine C6H13NO2 1.36 0.94 0.012 下降
    乙酰-L-酪氨酸 Acetyl-L-tyrosine C11H13NO4 1.38 0.87 0.010 下降
    γ-谷氨酰鸟氨酸 Gamma glutamyl ornithine C10H19N3O5 1.87 1.58 0.023 上升
    对茴香酸异戊酯 (异戊基异茴香酸酯) Isoamyl p-anisate C13H18O3 1.76 0.67 0.041 下降
    3-氧十二酸 3-Oxododecanoic acid C12H22O3 1.01 0.92 0.043 下降
    C-2神经酰胺 C-2 Ceramide C20H39NO3 2.21 0.75 0.028 下降
    磷脂酰乙醇胺 PE(15:0/16:1(9Z)) C36H70NO8P 1.42 1.10 0.030 上升
    半乳糖神经酰胺 Galactosyl ceramide (d18:1/14:0) C38H73NO8 1.97 2.78 0.045 上升
    N-棕榈酰甘氨酸 N-Palmitoyl glycine C18H35NO3 1.97 0.86 0.000 下降
    二十碳五烯酸 Eicosapentaenoic acid C20H30O2 1.01 0.98 0.004 下降
    溶血磷脂酰乙醇胺 LysoPE(0:0/20:2(11Z,14Z)) C25H48NO7P 1.33 1.12 0.037 上升
    9-羟基癸酸 9-Hydroxydecanoic acid C10H20O3 1.21 0.92 0.028 下降
    甘油一脂 MG(a-13:0/0:0/0:0)[rac] C16H32O4 1.92 0.79 0.040 下降
    反式-2-十二碳烯二酸 Traumatic acid C12H20O4 1.37 0.86 0.025 下降
    9-氧壬酸 9-Oxo-nonanoic acid C9H16O3 1.15 0.93 0.019 下降
    8-羟基-5,6-辛二烯酸 8-Hydroxy-5,6-octadienoic acid C8H12O3 1.04 0.94 0.016 下降
    (S)-3-磺酸盐 (S)-3-Sulfonatolactate C3H6O6S 2.57 3.05 0.046 上升
    γ-谷氨酰缬氨酸 gamma-Glutamylvaline C10H18N2O5 1.80 0.87 0.008 下降
    穗花牡荆苷 Agnuside C22H26O11 1.41 0.91 0.005 下降
    假尿苷 Pseudouridine C9H12N2O6 1.19 0.93 0.003 下降
    雌三醇7-(6-反式-对-香豆酰基葡萄糖苷)
    Eriodictyol 7-(6-trans-p-coumaroylglucoside)
    C30H28O13 1.44 0.92 0.020 下降
    注:FC表示某差异代谢物在淡水组相对于海水组的表达倍数变化,FC>1表示该代谢物上调,FC<1表示该代谢物下调;P<0.05表示差异显著,P<0.01表示差异极显著。 Note: FC value indicates the change of expression multiple of a differential metabolite in FOV group compared with SOV group. FC>1 indicates that the metabolite is upregulated; FC<1 indicates that the metabolite is down-regulated. P<0.05 indicates significant difference, and P<0.01 indicates extremely significant difference.
    下载: 导出CSV

    表  2  差异代谢产物的KEGG通路富集表

    Table  2.   KEGG pathway enrichment table of differential metabolites

    富集的差异代谢产物名称 
    Enriched differential metabolite name 
    富集个数
    Numble
    富集通路
    ID Pathway
    通路描述  
    Pathway desciption  
    P
    半乳糖神经酰胺 Galactosyl ceramide (d18:1/14:0) 1 map00600 鞘脂代谢 0.059
    脯氨酸 L-Proline 1 map00330 精氨酸和脯氨酸代谢 0.176
    异柠檬酸盐 Isocitrate 1 map00020 柠檬酸循环 0.057
    戊二酸 Glutaric acid 1 map00310 赖氨酸降解 0.130
    皮质醇 Cortisol 1 map00140 类固醇激素生物合成 0.207
    尿苷 Uridine;假尿苷 Pseudo uridine 2 map00240 嘧啶代谢 0.011
    异柠檬酸盐 Isocitrate 1 map00630 乙醛酸和二羧酸代谢 0.137
    L-异亮氨酸 L-Isoleucine;脯氨酸 L-Proline 2 map00970 氨酰tRNA生物合成 0.008
    L-异亮氨酸 L-Isoleucine 1 map00290 缬氨酸、亮氨酸和异亮氨酸生物合成 0.064
    二十碳五烯酸 Eicosapentaenoic acid 1 map01040 不饱和脂肪酸生物合成 0.097
    L-异亮氨酸 L-Isoleucine 1 map00280 缬氨酸、亮氨酸和异亮氨酸降解 0.107
    (S)-3-磺酸盐 (S)-3-Sulfonatolactate 1 map00270 半膀氨酸和蛋氨酸代谢 0.141
    下载: 导出CSV
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  • 收稿日期:  2021-06-24
  • 修回日期:  2021-10-09
  • 录用日期:  2021-11-04
  • 网络出版日期:  2021-11-11

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