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饲料中添加丁酸梭菌对卵形鲳鲹幼鱼生长性能和肠道菌群的影响

吴杨 杨铿 黄小林 周传鹏 徐创文 黄忠 虞为 荀鹏伟 黄健彬 麦晓勇 林黑着

吴杨, 杨铿, 黄小林, 周传鹏, 徐创文, 黄忠, 虞为, 荀鹏伟, 黄健彬, 麦晓勇, 林黑着. 饲料中添加丁酸梭菌对卵形鲳鲹幼鱼生长性能和肠道菌群的影响[J]. 南方水产科学. doi: 10.12131/20210141
引用本文: 吴杨, 杨铿, 黄小林, 周传鹏, 徐创文, 黄忠, 虞为, 荀鹏伟, 黄健彬, 麦晓勇, 林黑着. 饲料中添加丁酸梭菌对卵形鲳鲹幼鱼生长性能和肠道菌群的影响[J]. 南方水产科学. doi: 10.12131/20210141
Yang WU, Keng YANG, Xiaolin HUANG, Chuanpeng ZHOU, Chuangwen XU, Zhong HUANG, Wei YU, Pengwei XUN, Jianbin HUANG, Xiaoyong MAI, Heizhao LIN. Effects of dietary Clostridium butyricum supplementation on growth performance and intestinal flora of juvenile Trachinotus ovatus[J]. South China Fisheries Science. doi: 10.12131/20210141
Citation: Yang WU, Keng YANG, Xiaolin HUANG, Chuanpeng ZHOU, Chuangwen XU, Zhong HUANG, Wei YU, Pengwei XUN, Jianbin HUANG, Xiaoyong MAI, Heizhao LIN. Effects of dietary Clostridium butyricum supplementation on growth performance and intestinal flora of juvenile Trachinotus ovatus[J]. South China Fisheries Science. doi: 10.12131/20210141

饲料中添加丁酸梭菌对卵形鲳鲹幼鱼生长性能和肠道菌群的影响

doi: 10.12131/20210141
基金项目: 中国水产科学研究院基本科研业务费专项资金资助 (2020TD55);亚洲合作资金项目;中国水产科学研究院南海水产研究所中央级公益性科研院所基本科研业务费专项资金资助 (2021XK02);深圳市科技计划项目 (JCYJ20180306180203889);广东省现代农业产业技术体系创新团队建设专项资金 (2019KJ143)
详细信息
    作者简介:

    吴杨:吴 杨 (1997—),男,硕士研究生,研究方向为动物营养与饲料。E-mail: 13782920524@163.com

    通讯作者:

    林黑着 (1965—),男,博士,研究员,从事动物营养与饲料研究。E-mail: linheizhao@163.com

  • 中图分类号: S963.73+9

Effects of dietary Clostridium butyricum supplementation on growth performance and intestinal flora of juvenile Trachinotus ovatus

  • 摘要: 该试验研究了饲料中拌料添加丁酸梭菌 (Clostridium butyricum) 对卵形鲳鲹 (Trachinotus ovatus) 幼鱼生长性能和肠道菌群的影响,以期为丁酸梭菌在卵形鲳鲹养殖中的应用提供理论依据。设计了5组饲料,在基础饲料中分别添加0、1% (1×107 CFU·g−1)、2% (2×107 CFU·g−1)、3% (3×107 CFU·g−1)、4% (4×107 CFU·g−1) 的丁酸梭菌菌液饲喂卵形鲳鲹幼鱼8周。结果表明,饲料中添加2%丁酸梭菌可以显著提高卵形鲳鲹幼鱼的饲料利用率,肠道菌群群落的多样性最大,与对照组和4%添加组有显著差异 (P<0.05)。主坐标分析和主成分分析表明,饲料中丁酸梭菌的添加量是卵形鲳鲹幼鱼肠道菌群结构改变的主要因素。在门水平和属水平上,实验组与对照组均表现出不同程度的差异。饲料中添加丁酸梭菌能提高卵形鲳鲹幼鱼的生长性能和改善其肠道健康状态。
  • 图  1  卵形鲳鲹幼鱼增重率与饲料中丁酸梭菌添加量的关系

    Figure  1.  Relationship between weight gain rate of juvenile T. ovatus and amount of C. butyricum added in diet

    图  2  不同处理组卵形鲳鲹幼鱼肠道菌群稀释性曲线

    Figure  2.  Dilution curve of intestinal flora of juvenile T. ovatus in different treatment groups

    图  3  不同处理组卵形鲳鲹幼鱼肠道菌群的OTUs的venus图

    Figure  3.  Venus map of OTUs intestinal flora of juvenile T. ovatus in different treatment groups

    图  4  不同处理组卵形鲳鲹幼鱼肠道菌群主坐标分析

    Figure  4.  Principal co-ordinates analysis of intestinal flora of juvenile T. ovatus in different treatment groups

    图  5  不同处理组卵形鲳鲹幼鱼肠道菌群主成分分析

    Figure  5.  Principal component analysis of intestinal flora of juvenile T. ovatus in different treatment groups

    图  6  不同处理组卵形鲳鲹幼鱼肠道菌群在门水平上的相对丰度聚类堆叠图

    Figure  6.  Cluster stack diagram of relative abundance of intestinal flora of juvenile T. ovatus in different treatment groups at phylum level

    图  7  不同处理组卵形鲳鲹幼鱼肠道菌群在属水平上的相对丰度聚类堆叠图

    Figure  7.  Cluster stack diagram of relative abundance of intestinal flora of juvenile T. ovatus in different treatment groups at genus level

    表  1  饲料中添加丁酸梭菌对卵形鲳鲹幼鱼生长性能的影响

    Table  1.   Effects of dietary supplementation of C. butyricum on growth performance of juvenile T. ovatus

    项目
    Item
    组别 Group
    M0M1 (1%)M2 (2%)M3 (3%)M4 (4%)
    初均质量 IBM/g 9.63±0.15 9.47±0.21 9.43±0.21 9.63±0.12 9.60±0.20
    末均质量 FBW/g 21.80±0.62 22.73±0.95 23.00±1.14 23.03±0.31 21.33±1.12
    增重率 WGR/% 126.31±5.99ab 140.26±12.67ab 143.72±6.80a 139.13±4.66ab 122.45±16.22b
    特定生长率 SGR/(%·d−1) 4.08±0.13ab 4.38±0.26ab 4.45±0.14a 4.36±0.98ab 3.99±0.37b
    饲料系数 FCR 1.68±0.04a 1.51±0.12ab 1.49±0.09b 1.54±0.04ab 1.68±0.13a
    脏体比 VSI/% 6.10±0.83 6.19±0.59 6.59±0.23 6.35±0.33 6.47±0.22
    肝体比 HSI/% 2.17±0.12 1.95±0.21 2.19±0.21 2.13±0.19 2.24±0.09
    肥满度 CF/(g·cm−3) 3.40±0.19 3.29±0.29 3.65±0.15 3.48±0.18 3.38±0.07
    注:同行不同上标字母表示组间差异显著 (P<0.05),下表同此 Note: Values with different letters within the same column indicate significant difference (P<0.05). The same below.
    下载: 导出CSV

    表  2  饲料中添加丁酸梭菌对卵形鲳鲹幼鱼全鱼营养成分的影响 (干质量)

    Table  2.   Effects of dietary supplementation of C. butyricum on whole fish nutrient composition of juvenile T. ovatus (dry mass) %

    成分
    Composition
    组别 Group
    M0M1 (1%)M2 (2%)M3 (3%)M4 (4%)
    水分 Moisture 70.25±0.63 69.93±0.74 70.31±0.66 70.46±0.66 70.39±0.53
    粗蛋白 Crude protein 54.17±1.65 55.26±1.56 54.63±1.54 54.50±0.59 55.78±0.39
    粗脂肪 Crude lipid 30.87±2.55 31.21±1.42 31.04±1.60 31.41±0.45 30.28±0.43
    粗灰分 Ash 13.24±0.64 13.11±0.71 12.74±0.47 12.80±0.10 13.28±0.21
    下载: 导出CSV

    表  3  不同处理组卵形鲳鲹幼鱼肠道样品的多样性统计

    Table  3.   Diversity statistics of intestinal samples of juvenile T. ovatus in different treatment groups

    项目
    Item
    组别 Group
    M0M1 (1%)M2 (2%)M3 (3%)M4 (4%)
    OUT 39.00±7.55 34.33±7.02 30.33±2.08 41.00±6.08 36.33±8.08
    Ace 42.00±6.25 39.00±8.66 33.00±1.73 45.33±10.01 38.00±8.54
    Chao1 41.67±5.13 37.67±7.57 32.67±3.79 43.67±8.08 37.67±8.02
    Shannon 1.52±0.46a 1.79±0.02b 1.85±0.00b 1.80±0.01b 1.51±0.02a
    Simpson 0.29±0.01a 0.20±0.00b 0.19±0.00b 0.23±0.01c 0.33±0.01d
    下载: 导出CSV
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出版历程
  • 收稿日期:  2021-05-10
  • 修回日期:  2021-08-25
  • 录用日期:  2021-09-02
  • 网络出版日期:  2021-09-20

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