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牡蛎酶解超滤组分对TM4小鼠睾丸支持细胞的氧化损伤保护作用

张雪妍 秦小明 林海生 曹文红 郑惠娜 高加龙 章超桦

张雪妍, 秦小明, 林海生, 曹文红, 郑惠娜, 高加龙, 章超桦. 牡蛎酶解超滤组分对TM4小鼠睾丸支持细胞的氧化损伤保护作用[J]. 南方水产科学. doi: 10.12131/20210071
引用本文: 张雪妍, 秦小明, 林海生, 曹文红, 郑惠娜, 高加龙, 章超桦. 牡蛎酶解超滤组分对TM4小鼠睾丸支持细胞的氧化损伤保护作用[J]. 南方水产科学. doi: 10.12131/20210071
Xueyan ZHANG, Xiaoming QIN, Haisheng LIN, Wenhong CAO, Huina ZHENG, Jialong GAO, Chaohua ZHANG. Protective effect of hydrolyzed ultrafiltration fractions from oyster (Crassostrea hongkongensis) on oxidative damage of TM4 Sertoli cells[J]. South China Fisheries Science. doi: 10.12131/20210071
Citation: Xueyan ZHANG, Xiaoming QIN, Haisheng LIN, Wenhong CAO, Huina ZHENG, Jialong GAO, Chaohua ZHANG. Protective effect of hydrolyzed ultrafiltration fractions from oyster (Crassostrea hongkongensis) on oxidative damage of TM4 Sertoli cells[J]. South China Fisheries Science. doi: 10.12131/20210071

牡蛎酶解超滤组分对TM4小鼠睾丸支持细胞的氧化损伤保护作用

doi: 10.12131/20210071
基金项目: 财政部和农业农村部国家现代农业产业技术体系资助
详细信息
    作者简介:

    张雪妍 (1992—),女,博士研究生,研究方向为海洋功能活性物质。E-mail: 1162742815@qq.com

    通讯作者:

    秦小明 (1964—),男,博士,教授,从事水产品精深加工、贝类活性物质研究。E-mail: xiaoming0502@21cn.com

  • 中图分类号: TS 254.9

Protective effect of hydrolyzed ultrafiltration fractions from oyster (Crassostrea hongkongensis) on oxidative damage of TM4 Sertoli cells

  • 摘要: 该研究通过雷公藤甲素 (TP) 损伤TM4细胞构建生殖细胞损伤模型,探究了香港牡蛎 (Crassostrea hongkongensis) 酶解超滤组分对TP诱导的TM4小鼠 (Mus musculus) 睾丸支持细胞氧化损伤的保护作用,并检测了牡蛎酶解超滤组分的分子量分布与微量金属元素含量,比较分析了各个超滤组分对TP诱导的TM4细胞存活率以及还原型谷胱甘肽 (GSH)、丙二醇 (MDA) 和活性氧 (ROS) 水平的影响。结果表明,牡蛎酶解超滤组分富含铜 (Cu)、锌 (Zn)、锰 (Mn) 和硒 (Se) 等微量金属元素;经超滤分级,小分子与大分子物质得到有效分离;与模型组相比,各个超滤组分均不同程度地提高了经TP诱导的TM4细胞存活率,其中<3、3~5和5~10 ku超滤组分的细胞存活率高于>10 ku超滤组分;而<3 ku超滤组分可有效抵抗TP诱导的TM4细胞氧化应激损伤,减少细胞内ROS产生和脂质过氧化,增强TM4细胞的抗氧化能力。
  • 图  1  牡蛎酶解超滤组分作用于TM4细胞12 h (a) 和24 h (b) 后的细胞存活率

    与空白对照组相比,*. 显著差异 (P<0.05);**. 极显著差异 (P<0.01)。

    Figure  1.  Cell viability of oyster hydrolyzed ultrafiltration fractions after treatment on TM4 cells for 12 h (a) and 24 h (b)

    Compared with the control group, *. Significant difference (P<0.05); **. Very significant difference (P<0.01).

    图  2  雷公藤甲素对TM4细胞的毒性作用

    上标不同字母表示具有显著性差异 (P<0.05);图3图5同此。

    Figure  2.  Cytotoxic effect of TP on TM4 cells

    Different superscript letters indicate significant difference (P<0.05). The same case in Figure 3Figure 5.

    图  3  牡蛎超滤组分对雷公藤甲素诱导TM4细胞毒性的保护作用 (预处理时间:a. 6 h;b. 12 h)

    Figure  3.  Protective effect of oyster ultrafiltration fractions on TP-induced TM4 cytotoxicity (Pretreatment time: a. 6 h; b. 12 h)

    图  4  雷公藤甲素对TM4细胞内还原型谷胱甘肽和丙二醇含量的影响

    Figure  4.  Effect of TP on GSH and MDA content in TM4 cells

    图  5  牡蛎超滤组分对雷公藤甲素诱导TM4细胞内还原型谷胱甘肽和丙二醇含量的影响

    Figure  5.  Effect of oyster ultrafiltration fractions on GSH and MDA content in TM4 cells induced by TP

    图  6  DCFH-DA探针法检测TM4睾丸支持细胞内活性氧 (200×)

    Figure  6.  Intracellular ROS in TM4 Sertoli cells indicated as green fluorescence by DCFH-DA

    图  7  <3 ku牡蛎超滤组分对雷公藤甲素诱导TM4细胞内活性氧水平的影响

    与模型组相比,*. 显著差异 (P<0.05);**. 极显著差异 (P<0.01);与空白对照组相比,#. 显著差异 (P<0.05);##. 极显著差异 (P<0.01)。

    Figure  7.  Effect of <3 ku ultrafiltration fractions from oyster on TP-induced reactive oxygen species in TM4 cells

    Compared with the model group, *. Significant difference (P<0.05); **. Very significant difference (P<0.01). Compared with the control group, #. Significant difference (P<0.05); ##. Very significant difference (P<0.01).

    表  1  超滤膜分级后不同分子量区间的成分占各超滤组分的比值

    Table  1.   Ratio of different molecular mass ingredients to each ultrafiltration fraction after ultrafiltration          membrane classification       %

    超滤组分
    Ultrafiltration
    fraction/ku
    分子量 Molecular mass
    <33~55~10>10
    >1025.971.444.6367.96
    5~1036.321.298.5453.84
    3~546.791.2610.4241.53
    <357.482.659.1730.72
    下载: 导出CSV

    表  2  牡蛎超滤组分微量金属元素质量分数 

    Table  2.   Mass fractions of trace metal elements in oyster ultrafiltration fractions mg·kg–1

    超滤组分
    Ultrafiltration fraction/ku

    Cu

    Zn

    Mn

    Se
    >10771270119.73.96
    5~101918312828.33.69
    3~51952299825.44.08
    <31928301922.03.47
    下载: 导出CSV
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  • 收稿日期:  2021-03-04
  • 修回日期:  2021-03-22
  • 录用日期:  2021-04-28
  • 网络出版日期:  2021-05-10

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