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茜素络合物在青鱼幼鱼耳石上的标记效果评价

朱亚华 姜涛 陈修报 刘洪波 杨健

朱亚华, 姜涛, 陈修报, 刘洪波, 杨健. 茜素络合物在青鱼幼鱼耳石上的标记效果评价[J]. 南方水产科学. doi: 10.12131/20210048
引用本文: 朱亚华, 姜涛, 陈修报, 刘洪波, 杨健. 茜素络合物在青鱼幼鱼耳石上的标记效果评价[J]. 南方水产科学. doi: 10.12131/20210048
Yahua ZHU, Tao JIANG, Xiubao CHEN, Hongbo LIU, Jian YANG. Effect evaluation of alizarin complexone marking for otoliths of juvenile black carp (Mylopharyngodon piceus)[J]. South China Fisheries Science. doi: 10.12131/20210048
Citation: Yahua ZHU, Tao JIANG, Xiubao CHEN, Hongbo LIU, Jian YANG. Effect evaluation of alizarin complexone marking for otoliths of juvenile black carp (Mylopharyngodon piceus)[J]. South China Fisheries Science. doi: 10.12131/20210048

茜素络合物在青鱼幼鱼耳石上的标记效果评价

doi: 10.12131/20210048
基金项目: 中央级公益性科研院所基本科研业务费专项资金 (2019JBFM06);农业部财政专项 (CJDC-2017-22)
详细信息
    作者简介:

    朱亚华 (1995—),女,博士研究生,研究方向为渔业生态环境评价与保护。E-mail: 756620220@qq.com

    通讯作者:

    杨 健 (1964—),男,博士,研究员,从事渔业生态环境监测与保护研究。E-mail: jiany@ffrc.cn

  • 中图分类号: S 917.4

Effect evaluation of alizarin complexone marking for otoliths of juvenile black carp (Mylopharyngodon piceus)

  • 摘要: 放流标记技术研究是支撑增殖放流效果评估的重要内容。该研究用质量浓度为100 mg·L−1的茜素络合物对约2月龄的青鱼 (Mylopharyngodon piceus) 幼鱼进行48 h浸泡,随后移入正常的水体中恢复续养20 d。对其3种耳石在可见光和不同的激发光下进行比较分析,评价了茜素络合物在不同耳石上的沉积情况与标记效果。结果表明,通过在可见光和不同的激发光下进行观察,其3种耳石上均可见明显的标记区,标记率达100%。在对标记鱼恢复续养20 d后发现,耳石上的荧光标记区强度仍很高,表现出了较好的标记效果。此外,相关标记的最佳检测光源为蓝色激发光,绿色激发光次之。微耳石是用于青鱼幼鱼耳石荧光标记观察的最适类型,同时也是用于时滞检测的最适类型,其次是星耳石。此外,将鱼体浸泡入茜素络合物溶液中至其在耳石上形成标记沉积,以及将鱼体移出浸泡溶液至其耳石上标记区域消失,均存在1 d时滞。
  • 图  1  研究所用的青鱼幼鱼

    Figure  1.  Juvenile black carp in the study

    图  2  青鱼幼鱼的3类耳石

    a. 矢耳石;b. 微耳石;c. 星耳石

    Figure  2.  Three types of otoliths from juvenile black carp

    a. Sagitta; b. Lapillus; c. Asteriscus

    图  3  不同取样天数标记组和对照组青鱼幼鱼的体质量和全长变化 (n=5)

    *. 两组间差异显著 (P<0.05)

    Figure  3.  Change of body mass and total length of black carp of marking group and control group at different sampling day (n=5)

    *. Significant difference between two groups (P<0.05)

    图  4  经茜素络合物标记后恢复续养第10天时青鱼幼鱼矢耳石、微耳石、星耳石分别在可见光、蓝色激发光、绿色激发光和紫色激发光下的标记效果

    a1. 矢耳石分别在可见光、蓝色激发光、绿色激发光和紫色激发光下的标记效果;b1. 微耳石分别在可见光、蓝色激发光、绿色激发光和紫色激发光下的标记效果;c1. 星耳石分别在可见光、蓝色激发光、绿色激发光和紫色激发光下的标记效果。图中白色箭头表示由矢耳石、微耳石、星耳石核心指向荧光标记轮

    Figure  4.  Marking effect of three types of otoliths of juvenile black carp under different light sources at 10th day of recovery culture after ALC immersion

    a1. Marking effect of sagitta under visible light, blue excitation light, green excitation light and purple excitation light; b1. Marking effect of lapillus under visible light, blue excitation light, green excitation light and purple excitation light; c1. Marking effect of asteriscus under visible light, blue excitation light, green excitation light and purple excitation light. The white arrows show that the core of sagitta, lapillus and asteriscus point to the fluorescent marker ring.

    图  5  青鱼幼鱼经茜素络合物浸泡标记后恢复续养第0、第5、第10、第15和第20天时微耳石在蓝色激发光的标记效果

    Figure  5.  Marking effect of lapillus of juvenile black carp under blue light source at 0th, 5th, 10th, 15th, 20th day of recovery culture after ALC immersion

    图  6  青鱼幼鱼经茜素络合物浸泡标记后恢复续养第5天时微耳石标记效果

    2个白色箭头分别指向微耳石的2条紫红色标记环;黑色短线表示微耳石新形成的日轮所在位置,即为一明带和一暗带组合;4个黑色箭头则分别指向微耳石外部新形成的4个日轮

    Figure  6.  Marking effect of lapillus of juvenile black carp under four light sources at 5th day of recovery culture after ALC immersion

    Two white arrows point to two purple red marker rings in lapillus; the black dash shows the position of the newly formed daily growth increments in lapillus, which is a combination of a bright band and a dark band; the four black arrows point outward to the four newly formed daily growth increments in lapillus.

    表  1  青鱼幼鱼3类耳石在不同光源下的检测效果

    Table  1.   Detection effect of three types of otoliths from juvenile black carp under four different light sources

    恢复续养天数
    Days of recovery
    culture/d
    实验鱼数
    Number of
    fish
    耳石类型
    Otolith
    type
    耳石标本数
    Number of
    otolith
    标记强度 Marker intensity
    可见光
    BS
    蓝色激发光
    WBS
    绿色激发光
    WGS
    紫色激发光
    WUS
    0 5 矢耳石 10 2 (++) 2 (++) 2 (++) 1 (+)
    5 5 矢耳石 10 2 (++) 3 (+++) 3 (+++) 2 (++)
    10 5 矢耳石 10 2 (++) 4 (++++) 3 (+++) 2 (++)
    15 5 矢耳石 10 1 (+) 4 (++++) 3 (+++) 2 (++)
    20 5 矢耳石 10 1 (+) 3 (+++) 2 (++) 1 (+)
    0 5 微耳石 10 2 (++) 3 (+++) 3 (+++) 2 (++)
    5 5 微耳石 10 3 (+++) 4 (++++) 4 (++++) 3 (+++)
    10 5 微耳石 10 3 (+++) 4 (++++) 4 (++++) 3 (+++)
    15 5 微耳石 10 2 (++) 4 (++++) 4 (++++) 3 (+++)
    20 5 微耳石 10 2 (++) 4 (++++) 4 (++++) 2 (++)
    0 5 星耳石 10 2 (++) 2 (++) 2 (++) 1 (+)
    5 5 星耳石 10 3 (+++) 3 (+++) 2 (++) 1 (+)
    10 5 星耳石 10 3 (+++) 3 (+++) 2 (++) 1 (+)
    15 5 星耳石 10 3 (+++) 2 (++) 1 (+) 1 (+)
    20 5 星耳石 10 2 (++) 2 (++) 1 (+) 1 (+)
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出版历程
  • 收稿日期:  2021-01-27
  • 修回日期:  2021-04-07
  • 录用日期:  2021-04-30
  • 网络出版日期:  2021-05-15

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