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性类固醇激素雌二醇、睾酮对半滑舌鳎雌、雄、伪雄鱼生长性能的影响

王佳林 杨英明 杨倩 王娜 陈松林

王佳林, 杨英明, 杨倩, 王娜, 陈松林. 性类固醇激素雌二醇、睾酮对半滑舌鳎雌、雄、伪雄鱼生长性能的影响[J]. 南方水产科学. doi: 10.12131/20210030
引用本文: 王佳林, 杨英明, 杨倩, 王娜, 陈松林. 性类固醇激素雌二醇、睾酮对半滑舌鳎雌、雄、伪雄鱼生长性能的影响[J]. 南方水产科学. doi: 10.12131/20210030
Jialin WANG, Yingming YANG, Qian YANG, Na WANG, Songlin CHEN. Effects of sex steroid hormones (estradiol and testosterone) on growth traits of female, male and pseduo-male Chinese tongue sole (Cynoglossus semilaevis)[J]. South China Fisheries Science. doi: 10.12131/20210030
Citation: Jialin WANG, Yingming YANG, Qian YANG, Na WANG, Songlin CHEN. Effects of sex steroid hormones (estradiol and testosterone) on growth traits of female, male and pseduo-male Chinese tongue sole (Cynoglossus semilaevis)[J]. South China Fisheries Science. doi: 10.12131/20210030

性类固醇激素雌二醇、睾酮对半滑舌鳎雌、雄、伪雄鱼生长性能的影响

doi: 10.12131/20210030
基金项目: 国家自然科学基金项目 (31873037);中国水产科学研究院中央级公益性科研院所基本科研业务费专项资金资助 (2020TD20)
详细信息
    作者简介:

    王佳林 (1996—),男,博士研究生,研究方向为水产生物技术。E-mail: 529947529@qq.com

    通讯作者:

    王 娜 (1980—),女,博士,研究员,从事海水鱼类细胞培养及功能基因组学研究。E-mail: wangna@ysfri.ac.cn

  • 中图分类号: S 917.4

Effects of sex steroid hormones (estradiol and testosterone) on growth traits of female, male and pseduo-male Chinese tongue sole (Cynoglossus semilaevis)

  • 摘要: 多种鱼类包括半滑舌鳎 (Cynoglossus semilaevis) 都存在性别大小异形现象,这种生长二态性可能与性类固醇激素和生长轴相关基因的相互作用有关。笔者课题组前期已通过转录组学分析发现性类固醇激素可能参与半滑舌鳎性别大小异形调控过程。为探究性类固醇激素对半滑舌鳎不同性别生长差异的影响,该研究对16月龄雌、雄、伪雄鱼进行了雌二醇、睾酮激素注射处理,并对其生长数据进行统计,且对半滑舌鳎生长激素1 (Growth hormone 1, gh1)、胰岛素样生长因子1 (Insulin-like growth factor 1, igf1) 和细胞因子信号转导抑制因子 (Suppressor of cytokine signaling 3, socs3) 3个基因进行了定量分析。结果表明,两种性类固醇激素对半滑舌鳎不同性别个体的生长均具有抑制作用。激素处理后,gh1、igf1在脑、肝脏中的表达量总体呈下降趋势,socs3在肌肉和肝脏中的表达量显著上升。该研究探讨了性类固醇激素对半滑舌鳎不同性别生长性能的影响,为深入研究性固醇激素调控鱼类性别大小异形现象提供了参考。
  • 图  1  半滑舌鳎伪雄鱼鉴定电泳结果 (部分)

    ZW. 伪雄鱼;ZZ. 雄鱼;M. DL2000 DNA Marker

    Figure  1.  Electrophoresis results of pseudo-male identification of C. semilaevis (partial)

    ZW. Pseudo males; ZZ. Males; M. DL2000 DNA Marker

    图  2  性类固醇激素处理后体质量绝对生长率对比

    C. 对照组;E. 雌二醇处理组;T. 睾酮处理组;标有不同字母表示差异显著 (P<0.05),下同

    Figure  2.  Comparison of absolute growth rate of bodymass after treatment with sex steroid hormones

    C. Control group; E. Estradiol treated group; T. Testosterone treated group; different letters indicate significant difference (P<0.05). The same below.

    图  3  性类固醇激素处理后体长绝对生长率对比

    Figure  3.  Comparison of absolute growth rate of body length after treatment with sex steroid hormones

    图  4  睾酮、雌二醇处理后gh1 mRNA在半滑舌鳎不同性别脑组织中表达量的变化

    Figure  4.  Change of gh1 mRNA expression in brain tissues of different sexes of C. semilaevis after T and E2 treatments

    图  5  睾酮、雌二醇处理后igf 1 mRNA在半滑舌鳎不同性别肝脏中表达量的变化

    Figure  5.  Change of igf 1 mRNA expression in liver of different sexes of C. semilaevis after T and E2 treatments

    图  6  睾酮、雌二醇处理后socs3 mRNA在半滑舌鳎不同性别肝脏中表达量的变化

    Figure  6.  Change of socs3 mRNA expression in liver of different sexes of C. semilaevis after T and E2 treatments

    图  7  睾酮、雌二醇处理后socs3 mRNA在半滑舌鳎不同性别肌肉中表达量的变化

    Figure  7.  Changes of socs3 mRNA expression in muscle of different sexes of C. semilaevis after T and E2 treatments

    表  1  荧光定量PCR分析所用引物

    Table  1.   Primers for real-time PCR

    NCBI 检索号
    NCBI No.
    引物
    Primer
    序列 (5'−3')
    Primer sequence
    103393304 β-actin-F GCTGTGCTGTCCCTGTA
    β-actin-R GAGTAGCCACGCTCTGTC
    103387754 gh1-F CCGAAGTGAACTGAAGAAA
    gh1-R CTGAGCAAGCAGGTGAAG
    103382578 igf1-F TCGCATCTCATCCTCTTTCTT
    igf1-R ATACAGCACATCGCACTCTTG
    103381769 socs3-F GTCCGACCAGATTTAGCC
    socs3-R TACCTTTGCCACTCACATAA
    下载: 导出CSV

    表  2  对照组、E处理组和T处理组生长参数比较

    Table  2.   Comparison of growth parameters between control group, E treatment group and T treatment group $\overline { X}{\bf \pm {SD}}$; n=30

    组别
    Group
    性别
    Gender
    初始体质量
    Initial body
    mass/g
    实验后体质量
    Body mass
    after treatment/g
    初始体长
    Initial body
    length/cm
    实验后体长
    Body length
    after treatment/
    cm
    体质量绝对
    生长率
    Absolute
    growth
    rate W/%
    体长绝对
    生长率
    Absolute
    growth
    rate L/%
    对照组 Control group 雌 F 734.38±83.84a 775.00±77.47a 48.67±2.56a 51.63±2.50a 5.53±0.08c 2.73±0.03b
    雄 M 134.39±19.99b 148.93±20.37b 30.26±1.67b 30.38±1.35b 10.82±0.02a 0.41±0.19d
    伪雄 PM 148.90±22.28b 165.24±21.48b 29.60±2.14b 31.33±1.26b 10.97±0.04a 5.84±0.41a
    E处理组 E treatment group 雌 F 778.76±94.05a 788.46±73.24a 48.97±2.81a 51.56±2.71a 1.25±0.10f 1.96±0.06c
    雄 M 133.75±20.57b 137.52±19.99b 29.80±1.07b 29.95±1.39b 2.82±0.02e 0.52±0.04d
    伪雄 PM 154.83±27.14b 166.73±30.73b 30.18±2.40b 30.41±2.16b 7.69±0.06b 0.75±0.11d
    T处理组 T treatment group 雌 F 721.45±69.85a 750.00±78.54a 48.35±2.07a 51.24±2.15a 3.96±0.22d 2.59±0.04b
    雄 M 147.16±14.76b 152.70±19.03b 30.10±1.47b 30.47±1.53b 3.76±0.03d 1.23±0.29c
    伪雄 PM 159.72±26.19b 165.52±24.65b 30.18±1.90b 30.70±2.11b 3.63±0.13d 1.70±0.10c
    注:同一处理组、不同性别鱼之间的比较或同一性别鱼、不同处理组之间的比较;标有不同字母表示差异显著 (P<0.05) Note: Comparison between fish of the same treatment group and different sexes or comparison between fish of the same sex and different treatment groups. Different letters indicate significant difference (P<0.05).
    下载: 导出CSV
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  • 收稿日期:  2021-01-15
  • 修回日期:  2021-03-29
  • 录用日期:  2021-04-07
  • 网络出版日期:  2021-04-16

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