Abstract: In this paper, we investigated the effects of water temperature, photoperiod and light intensity on the survival, feeding and growth of Schizothorax biddulphi juveniles by using experimental ecological methods; predicted the optimal growth conditions for the juveniles by Box-Benhnken design; and studied the saline alkali tolerance of juveniles by single factor static toxicity acute test. The results show that the survival rate of the juveniles was above 85% at 5−25 ℃. The feeding rate, length growth rate and specific growth rate reached the maximum values at 20 ℃. When the photoperiod was 16 L∶8 D and the light intensity was 500 lx, all the indices of the juveniles reached the maximum values. Under conditions of water temperature of 12.98 ℃, daily light time of 15.07 h and light intensity of 670.27 lx, the best survival, feeding and growth were obtained. The LC₅₀ of NaCl salinity was 4.01, 3.62, 3.29 and 2.92 g·L⁻¹ at 24, 48, 72 and 96 h, respectively, and the safety concentration (SC) was 0.89 g·L⁻¹. The LC₅₀ of NaHCO₃ alkalinity was 97.14, 86.75, 80.26 and 74.52 mmol·L⁻¹ at 24, 48, 72 and 96 h, respectively, and the SC was 20.75 mmol·L⁻¹.