廖庆钊, 陈福艳, 覃雅, 黄婵婵, 黄罗冬, 申佩弘. 投喂乙醇假丝酵母对罗非鱼生长、免疫和肠道菌群的影响[J]. 南方水产科学, 2021, 17(5): 10-17. DOI: 10.12131/20200258
引用本文: 廖庆钊, 陈福艳, 覃雅, 黄婵婵, 黄罗冬, 申佩弘. 投喂乙醇假丝酵母对罗非鱼生长、免疫和肠道菌群的影响[J]. 南方水产科学, 2021, 17(5): 10-17. DOI: 10.12131/20200258
Qingzhao LIAO, Fuyan CHEN, Ya QIN, Chanchan HUANG, Luodong HUANG, Peihong SHEN. Effects of feeding Candida ethanolica GXU01 on growth, immunity and intestinal flora of tilapia[J]. South China Fisheries Science, 2021, 17(5): 10-17. DOI: 10.12131/20200258
Citation: Qingzhao LIAO, Fuyan CHEN, Ya QIN, Chanchan HUANG, Luodong HUANG, Peihong SHEN. Effects of feeding Candida ethanolica GXU01 on growth, immunity and intestinal flora of tilapia[J]. South China Fisheries Science, 2021, 17(5): 10-17. DOI: 10.12131/20200258

投喂乙醇假丝酵母对罗非鱼生长、免疫和肠道菌群的影响

Effects of feeding Candida ethanolica GXU01 on growth, immunity and intestinal flora of tilapia

  • 摘要: 为探究乙醇假丝酵母 (Candida ethanolica GXU01) 在罗非鱼养殖中的益生潜能,为罗非鱼可持续养殖寻找生态友好的饲用添加菌,该研究将乙醇假丝酵母作为饲料添加菌饲喂尼罗罗非鱼 (Oreochroms niloticus),通过测定罗非鱼的生长性能、肠道消化酶、血清非特异性免疫指标、肠道微生物群落结构以及用无乳链球菌 (Streptococcus agalactiae) 对罗非鱼进行攻毒试验,全面评价了乙醇假丝酵母对罗非鱼生长及其免疫力的影响。结果表明,摄食乙醇假丝酵母可以使罗非鱼的生长性能、消化酶活性、血清溶菌酶活性和补体C3含量显著提高 (P<0.05)。投喂乙醇假丝酵母后,罗非鱼肠道中梭杆菌门、鲸杆菌属 (Cetobacterium) 和艾克曼菌属 (Akkermansia) 等有益菌群的丰度显著上调,蓝细菌门大幅减少。攻毒试验中,摄食酵母饲料的罗非鱼成活率增加26.66%。研究表明乙醇假丝酵母对罗非鱼肠道消化能力和免疫抗病能力均有促进作用。

     

    Abstract: To explore the probiotic potential of Candida ethanolica GXU01 in tilapia culture and to find ecological and friendly feeding bacteria for tilapia sustainable cultivation, we used C. ethanolica GXU01 as feed-additive to feed tilapia. Then we determined the growth performance, intestinal digestive enzymes, serum non-specific immune indexes and intestinal microbial community structure, and challenged the tilapia with Streptococcus agalactiae, so as to comprehensively evaluate the effects of C. ethanolica GXU01 on the growth and immunity of tilapia. The results show that the growth performance, digestive enzyme activity, serum lysozyme activity and complement C3 content of tilapia could be significantly improved by feeding C. ethanolica GXU01 (P<0.05). The abundance of Fusobacteria, Cetobacterium and Akkermansia in the intestinal tract of tilapia increased significantly and those of Cyanobacteria decreased significantly after feed of C. ethanolica GXU01. In the challenge test, the survival rate of tilapia fed with diet containing C. ethanolica GXU01 increased by 26.66%. The study shows that C. ethanolica GXU01 promotes the intestinal digestive ability and immune resistance of tilapia.

     

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