Correlation analysis of light intensity and growth, photosynthetic pigment, color value of Betaphycus gelatinae
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摘要: 文章探讨了不同光照强度 (1 000、3 000、5 000、7 000和9 000 lx) 下琼枝藻 (Betaphycus gelatinae) 的生长、光合色素及颜色变化，基于CSE-1成像色度检测分析系统，研究了琼枝藻颜色参数L*a*b* (CIE 1976) 与光照强度、生长、光合色素的相关性。结果显示，琼枝藻的相对生长速率和增重率均随光照强度的增加显著增大，适宜生长的光照强度为7 000~9 000 lx，光照强度为1 000 lx时琼枝藻无明显生长。叶绿素a (Chl-a)、类胡萝卜素 (Car)、藻红蛋白 (PE) 和藻蓝蛋白 (PC) 含量总体上随光照强度的增加呈下降趋势。随着光照强度的增加，琼枝藻的颜色由红褐色逐渐变为绿色，三刺激值XYZ在CIE 1931色度图上呈现明显的分布差异。光照强度与明度 (L*) 呈显著正相关 (P<0.05)，与红绿色度 (a*) 呈极显著负相关 (P<0.01)；生长速率与L*、黄蓝色度 (b*) 均呈显著正相关 (P<0.05)；Chl-a与L*呈显著负相关 (P<0.05)，与a*呈显著正相关 (P<0.05)；PE和PC均与a*呈显著正相关 (P<0.05)。Abstract: We investigated the growth, photosynthetic pigment and color change of Betaphycus gelatinae at different light intensities (1 000, 3 000, 5 000, 7 000 and 9 000 lx). Based on CSE-1 imaging chromaticity detection and analysis, we studied the correlation of color parameter L*a*b*(CIE 1976) with light intensity, growth and photosynthetic pigment. The results show that the relative growth rate and weight gain rate increased significantly with the increase of light intensity. The suitable light intensity for growth was 7 000−9000 lx. However, when the light intensity was 1 000 lx, there was no obvious growth of B. gelatinae. With the increase of light intensity, the contents of chlorophyll a, carotenoids, phycoerythrin and phycocyanin generally decreased, and the color of B. gelatinae gradually changed from reddish brown to green. The tristimulus values (XYZ) showed a significant distribution difference on the CIE 1931 chromaticity diagram at different light intensities. Light intensity was significantly positively correlated with lightness L*(P<0.05), but significantly negatively correlated with red-green value a*(P<0.01). There was a significant positive correlation between relative growth rate with lightness L* and yellow-blue value b*(P<0.05). Chlorophyll a was significantly negatively correlated with lightness L*(P<0.05), but significantly positively correlated with red-green value a*(P<0.05). Both phycoerythrin and phycocyanin were significantly positively correlated with the red-green value a*(P<0.05).
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Key words:
- Betaphycus gelatinae /
- Light intensity /
- Growth /
- Photosynthetic pigment /
- Color parameters /
- Correlation analysis
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图 1 不同光照强度对琼枝藻相对生长速率的影响
不同小写字母间表示差异显著 (P<0.05)。
Figure 1. Effects of different light intensities on RGR of B. gelatinae
Different lowercase letters indicate significant difference (P<0.05).
图 2 不同光照强度下琼枝藻增重率随时间的变化
Figure 2. Change of WGR for B. gelatinae at different light intensities along with time
图 3 不同光照强度下琼枝藻的色素质量分数
不同小写字母间表示差异显著 (P<0.05)；不同大写字母间表示差异显著 (P<0.05)；图4同此。
Figure 3. Pigment mass fraction of B. gelatinae at different light intensities
Different lowercase letters indicate significant difference (P<0.05). Different capital letters indicate significant difference (P<0.05). The same case in Figure 4.
图 4 不同光照强度下琼枝藻的藻胆蛋白质量分数
Figure 4. Phycobiliprotein mass fraction of B. gelatinae at different light intensities
图 5 不同光照强度下琼枝藻的颜色变化
Figure 5. Color change of B. gelatinae at different light intensities
图 6 不同光照强度下琼枝藻的颜色色度图分布
Figure 6. Distribution of XYZ on CIE color diagram for B. gelatinum at different light intensities
表 1 不同光照强度下琼枝藻的明度、红绿色度和黄蓝色度
Table 1. L*a*b* values of B. gelatinae at different light intensities
光照强度
Light intensity/lx数量
Number明度 L*
Lightness value红绿色度 a*
Red-green value黄蓝色度 b*
Yellow-blue value1 000 15 26.14±5.10a 28.24±5.98a 12.49±3.68a 3 000 15 37.73±6.37b 16.88±2.17b 29.67±3.75bc 5 000 15 45.57±8.98cd 6.21±3.06c 26.97±4.68b 7 000 15 43.31±2.56bc −1.29±3.99d 35.01±4.51d 9 000 15 50.64±4.58d −10.28±2.41e 34.25±5.74cd 注：同列不同字母间存在显著性差异 (P<0.05)。 Note: Values with different letters within the same column indicate significant difference (P<0.05). 
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表 2 不同光照强度下琼枝藻颜色的色差∆Eab
Table 2. ∆Eab values of B. gelatinae at different light intensities
光照强度
Light intensity/lx1 000 3 000 5 000 7 000 3 000 23.63 5 000 32.75 13.51 7 000 40.92 19.75 11.22 9 000 50.57 30.42 18.72 11.62
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表 3 琼枝藻的颜色参数 (明度、红绿色度、黄蓝色度) 与光照强度、相对生长速率、光合色素的相关性
Table 3. Correlation between L*a*b* values with light intensity, RGR and photosynthetic pigment
参数
Parameter光照强度
Light intensity相对生长速率
RGR叶绿素a
Chl-a类胡萝卜素
Car藻红蛋白
PE藻蓝蛋白
PC明度 L* Lightness value 0.922* 0.933* −0.883* −0.389 −0.863 −0.857 红绿色度 a* Red-green value −0.997** −0.868 0.952* 0.558 0.910* 0.908* 黄蓝色度 b* Yellow-blue value 0.848 0.968** −0.712 −0.087 −0.682 −0.686 注：*. 显著差异 (P<0.05)；**. 极显著差异 (P<0.01)。
Note: *. Significant difference (P<0.05); **. Very significant difference (P<0.01).
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