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渔用氧化剂对水源水和池塘水中磺胺类抗性基因sul1的去除作用

赵晓雨 苏浩昌 徐煜 徐武杰 胡晓娟 文国樑 曹煜成 余招龙

赵晓雨, 苏浩昌, 徐煜, 徐武杰, 胡晓娟, 文国樑, 曹煜成, 余招龙. 渔用氧化剂对水源水和池塘水中磺胺类抗性基因sul1的去除作用[J]. 南方水产科学, 2021, 17(3): 46-53. doi: 10.12131/20200231
引用本文: 赵晓雨, 苏浩昌, 徐煜, 徐武杰, 胡晓娟, 文国樑, 曹煜成, 余招龙. 渔用氧化剂对水源水和池塘水中磺胺类抗性基因sul1的去除作用[J]. 南方水产科学, 2021, 17(3): 46-53. doi: 10.12131/20200231
Xiaoyu ZHAO, Haochang SU, Yu XU, Wujie XU, Xiaojuan HU, Guoliang WEN, Yucheng CAO, Zhaolong YU. Removal of sulphonamide resistance sul1 gene in water source and pond water by fishery oxidants in aquaculture[J]. South China Fisheries Science, 2021, 17(3): 46-53. doi: 10.12131/20200231
Citation: Xiaoyu ZHAO, Haochang SU, Yu XU, Wujie XU, Xiaojuan HU, Guoliang WEN, Yucheng CAO, Zhaolong YU. Removal of sulphonamide resistance sul1 gene in water source and pond water by fishery oxidants in aquaculture[J]. South China Fisheries Science, 2021, 17(3): 46-53. doi: 10.12131/20200231

渔用氧化剂对水源水和池塘水中磺胺类抗性基因sul1的去除作用

doi: 10.12131/20200231
基金项目: 国家重点研发计划项目 (2019YFD0900402);中国水产科学研究院基本科研业务费专项资金 (2020TD54);财政部和农业农村部国家现代农业产业技术体系资助 (CARS-48);广东省现代农业产业技术体系创新团队建设专项资金 (2019KJ149)
详细信息
    作者简介:

    赵晓雨 (1996—),男,硕士研究生,研究方向为养殖环境安全。E-mail: 13576943750@163.com

    通讯作者:

    曹煜成 (1979—),男,博士,副研究员,从事水产健康养殖和养殖生态环境调控研究。E-mail: cyc_715@163.com

  • 中图分类号: S 949

Removal of sulphonamide resistance sul1 gene in water source and pond water by fishery oxidants in aquaculture

  • 摘要: 为探讨利用渔用氧化剂去除养殖水体环境中的抗生素抗性基因 (Antibiotic resistant genes, ARGs) 并控制其传播的可行性,该研究以磺胺类抗性基因sul1作为目标抗性基因,选用次氯酸钠 (NaClO)、二溴海因 (C5H6Br2N2O2) 和高锰酸钾 (KMnO4) 3种养殖中常用的渔用氧化剂,运用实时荧光定量PCR技术,分析目标渔用氧化剂对水源水和池塘水体中ARGs的去除效果。结果显示,测试的水源水和池塘水体中的sul1浓度均处于较高水平,分别达到了105和106 拷贝·mL−1,而且微生物的细胞内sul1是其主要存在形式;其次,NaClO对各实验组中的sul1均呈现较强的去除效果,C5H6Br2N2O2仅对水源水中细胞内和细胞外的sul1具有较强的去除作用,KMnO4仅对不同水体中的细胞外sul1具有较强的去除作用。结果表明,3种目标渔用氧化剂中,NaClO对水源水和池塘水体中ARGs的去除效果最好,科学应用NaClO有利于去除水产养殖水环境中的ARGs,进而防控其在养殖过程中的传播。
  • 图  1  消毒前后水源水中i-sul1 (a) 和e-sul1 (b) 浓度的变化

    同一图中标有不同字母代表有显著差异 (P<0.05);图2同此

    Figure  1.  Change in concentrations of i-sul1 (a) and e-sul1 (b) in water source before and after disinfection

    Different letters in the same figure indicate significant difference (P<0.05). The same case in Figure 2.

    图  2  消毒前后池塘水中i-sul1 (a) 和e-sul1 (b) 浓度的变化

    Figure  2.  Change in concentrations of i-sul1 (a) and e-sul1 (b) in pond water before and after disinfection

    图  3  消毒过程中活性氯质量浓度 (a) 及其所达到的浓时积 (b) 的变化

    Figure  3.  Change in concentration of FAC (a) and reached CT value (b) during disinfection

    图  4  消毒过程中高锰酸钾质量浓度 (a) 及其所达到的浓时积 (b) 的变化

    Figure  4.  Change in concentration of KMnO4 (a) and reached CT value (b) during disinfection

    表  1  水源水和池塘水中氨氮、亚硝酸盐氮和总有机碳质量浓度

    Table  1.   Concentrations of NH4 +-N, NO2 -N and TOC in water source and pond water mg·L−1

    项目
    Item
    水源水
    Water source
    池塘水
    Pond water
    氨氮 NH4 +-N 0.127 1.120
    亚硝酸盐氮 NO2 -N 0.013 0.845
    总有机碳 TOC 8.9 10.9
    下载: 导出CSV

    表  2  qPCR所需引物

    Table  2.   Primers used for quantitative PCR

    基因
    Gene
    引物对
    Primer pair
    序列 (5'—3')
    Sequence
    退火温度
    Annealing temperature/℃
    片段大小
    Amplicon size/bp
    参考文献
    Reference
    sul1FWCGCACCGGAAACATCGCTGCAC62163[23]
    RVTGAAGTTCCGCCGCAAGGCTCG
    注:FW. 上游引物;RV. 下游引物 Note: FW. Forward primer; RV. Reverse primer
    下载: 导出CSV
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出版历程
  • 收稿日期:  2020-11-05
  • 修回日期:  2021-03-08
  • 录用日期:  2021-03-15
  • 网络出版日期:  2021-04-13
  • 刊出日期:  2021-06-05

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